Colloidal gold immunity test strip for rapidly detecting rice stripe virus (RSV) and preparation method

Abstract

The invention discloses a colloidal gold immunity test strip for rapidly detecting a rice stripe virus (RSV) and a preparation method. According to the invention, an anti-RSV monoclonal antibody is labeled by colloidal gold into a gold-labeled antibody, the monoclonal antibody is wrapped by a combination pad made of a polyester film, another anti-RSV monoclonal antibody and goat anti-mouse IgG arecombined on a detection line (T line) and a quality control line (C line) of a nitrocellulose membrane respectively, and then a sample pad made of water absorbing filter paper, an RSV immune colloidal gold pad, the nitrocellulose membrane combined with the RSV monoclonal antibody 11C1 and the goat anti-mouse IgG and the water absorbing paper are sequentially pasted on a PVC rubber slab, so that the test strip is prepared. The test strip can detect the RSV in field rice and single Laodelphax striatellus samples accurately, specially and sensitively. According to the test strip, people can directly observe a detection result with the naked eyes within 3-5min. The test strip is suitable for detecting large samples in the field, and provides technical and material support for the diagnosis, early monitoring and early warning, disease resistance breeding, epidemiological research and scientific prevention and control of rice stripe diseases.

Description

technical field [0001] The field of the invention belongs to the field of biotechnology, and in particular relates to a colloidal gold immune test strip for rapidly detecting rice stripe virus and a preparation method thereof. Background technique [0002] Widely distributed in Asia, especially in Japan, China and other regions, rice stripe blight is caused by rice stripe virus (Rice stripe virus, RSV) transmitted by the small planthopper. The virus directly infects rice seedlings Lead to the death of rice seedlings. Although the infected rice in the later stage is not dead, it destroys the reproductive growth of rice, causes abnormal heading, and is dominated by grains, seriously affecting rice production. Therefore, this virus disease is an important virus that threatens rice production in China Disease. Rice stripe virus is a typical member of the genus Leprovirus, which is transmitted by eggs in a persistent manner and through eggs, and can infect more than 20 kinds of g...

AI Technical Summary

Problems solved by technology

The accuracy of field symptom observation is low, and rice and other plants have no symptoms in the early stage of virus infection. Both RT-PCR and electron microscope observation require sophisticated instruments and professional operators, and are only suitable for the detection of small samples in the laboratory

At present, only the dot-ELISA serological method is suitable for the detection of large quantities of samples in the field, but it still takes a long time, while the colloidal gold immunoassay strip is easy to operate, does not require any equipment, and the sample detection is completed within 3-5 minutes. Judging by visual observation, it is very suitable for grassroots units and farmers' field applications

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