Method for determining contents of 21 types of terpenoids in tobacco leaf and application of method
A technology of terpenoids and content, which is applied in the field of determination of 21 kinds of terpenoids in tobacco leaves, can solve the problems of complex pretreatment and narrow coverage of terpenoids, and achieve the effect of good selectivity, high sensitivity and low interference
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Embodiment 1
[0031] Such as figure 1 Shown, the method for the content of 21 kinds of terpenoids in the assay tobacco leaf of the present embodiment comprises the following steps:
[0032] 1. Determination of the standard curve:
[0033] Accurately take by weighing 21 kinds of terpenoid standard items that the present invention measures, dissolve with the dichloromethane solution containing tridecanoic acid (the concentration of tridecanoic acid is 2.5mg / L), be mixed with the mass concentration of each terpenoid Both are 1mg / L standard stock solutions, which are stored at -80°C for future use.
[0034] Then accurately draw the above-mentioned standard stock solution, dilute with a dichloromethane solution containing tridecanoic acid (the concentration of tridecanoic acid is 2.5mg / L) to prepare a series of standard working solutions, and then analyze with GC-MS / MS. Among them, the analysis conditions of GC-MS / MS are as follows: the chromatographic column is DB-5MS gas chromatographic colu...
Embodiment 2
[0047] According to the determination method of Example 1, the terpenoid components in the tobacco variety Yun 87 in Yunnan area were detected, and the measured results are shown in Table 3.
[0048] Table 3 Contents of 21 terpenoids in tobacco leaves of Yun 87 in Yunnan area
[0049]
[0050]
[0051] *Cannot be detected in normal tobacco leaves.
[0052] In other embodiments of the present invention, other tobacco leaf varieties in Yunnan area (such as Kunming Hongda, Qujing Yun 105, etc.), tobacco leaf varieties in Henan area (Xuchang Yuyan No. 10, Pingdingshan Zhongyan 100, Nanyang Zhongyan 100 and The content of terpenoids in Zhumadian Zhongyan 100) and Guizhou tobacco varieties (Zunyi Nanjiang No. 3, Bijie Bina No. 1, Qiannan Guiyan 201 and Tongren K326) were detected and analyzed.
[0053] In other embodiments of the present invention, 1.0-2.0 mL of extract can be added for every 20 mg of fresh tobacco leaf freeze-dried powder. The extraction solution was a dich...
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