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Reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper

A hydroxybutyrate dehydrogenase, dry chemical technology, applied in the field of in vitro diagnostic products, can solve the problems of inconvenient use of liquid biochemical reagents, and achieve the effects of less pollution, good uniformity and convenient operation

Pending Publication Date: 2019-04-12
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on this, it is necessary to provide a kind of reaction solution and dry chemical test paper for making α-hydroxybutyrate dehydrogenase assay dry chemical test paper, to solve the inconvenience of using the liquid biochemical reagent of traditional α-hydroxybutyrate dehydrogenase The problem

Method used

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  • Reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper
  • Reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper
  • Reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Use the prepared chromogen solution to make the reagent layer, and use the prepared reaction solution to make the chromogenic layer; dry them separately and put them away. The upper support layer and the lower support layer are made of polyvinyl chloride (PVC) sheets, and the sample hole and the detection hole are respectively punched out with a punching machine. The diameter of the hole is 5mm. Synthetic film with a width of 3 mm, the diffusion layer and the color development layer are pasted on the upper support layer and the lower support layer respectively, and are located at the sample injection hole and the test hole. Then the reagent layer is sandwiched between the upper support layer pasted with the diffusion layer and the lower support layer pasted with the chromogenic layer, the positions of the sample hole and the test hole are aligned, and the upper support layer and the lower support layer are pasted together.

[0053] The formula of reaction solution is as...

Embodiment 2

[0061] Use the prepared chromogen solution to make the reagent layer, and use the prepared reaction solution to make the chromogenic layer; dry them separately and put them away. The upper support layer and the lower support layer adopt PET plastic sheets, and punch out sample injection holes and detection holes in the same manner as in Example 1. The diameter of the holes is 6mm, and the hole distance is 8mm. The diffusion layer adopts non-woven fabrics, and the reagent layer adopts glass fibers. The width is 8mm, the diffusion layer and the color layer are pasted on the upper support layer and the lower support layer respectively, and then the upper support layer and the lower support layer are clamped with the reagent layer, and then pasted together, the sample hole and the test hole position alignment.

[0062] The formula of reaction solution is as follows:

[0063]

[0064] When the reagent layer and the chromogenic layer are dried, they are placed in a vacuum oven a...

Embodiment 3

[0070] Determination of whole blood samples. The selection of bottom plate material and processing specification is the same as in embodiment 1. Paste the blood filter material glass fiber in the middle of the diffusion layer and the reagent layer. The whole blood sample is added at the mesh cloth of the sample hole. Other making raw materials and method of dry chemical test paper are with embodiment 1.

[0071] Whole blood samples of fixed value were taken, and the sample volume was 30 μl each time, the reaction slope was measured, and the linear regression equation was calculated. The results are shown in Table 3 below.

[0072]table 3

[0073]

[0074] The resulting linear regression equation is Y=51865X+24.04, R 2 =0.991, linear in the range of 50-900IU / L.

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Abstract

The invention discloses reaction liquid for preparing dry chemical test paper for determination of alpha-hydroxybutyrate dehydrogenase, and dry chemical test paper. The dry chemical test paper comprises a reaction chromogenic layer containing alpha-hydroxybutyric acid, coenzyme I, diaphorase and a chromogenic reagent capable of generating a chromogenic reaction with NADH under the catalysis of diaphorase. The dry chemical test paper prepared by the reaction liquid can be used in combination with a small instrument to determine the content of alpha-hydroxybutyrate dehydrogenase in a short period of time only by collecting a small amount of blood, the operation is simple and convenient, no professional operation is required, the intensity of color development is high, the uniformity is good,the pollution is small, the detection process does not rely on a large-scale biochemical analyzer, the market requirements under special conditions can be met, especially emergency treatment, and compared with a traditional method for detecting by adopting a liquid biochemical reagent, the dry chemical test paper can provide a method capable of conveniently and quickly measuring the activity of alpha-hydroxybutyrate dehydrogenase for the emergency department, primary hospitals, families and small clinics.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic products, in particular to a reaction solution and a dry chemical test paper for making α-hydroxybutyrate dehydrogenase assay dry chemical test paper. Background technique [0002] α-Hydroxybutyrate dehydrogenase (α-HBDH, EC1.1.1.30) mainly comes from myocardium, kidney and red blood cells, more in myocardium and less in liver. α-Hydroxybutyrate dehydrogenase is not an independent specific enzyme, but a general term for lactate dehydrogenase isoenzymes LDH1 and LDH2 containing H subunits, which are commonly found in the human body, and the normal reference range of the human body is (33°C ): 90-220U / L (enzyme rate method). Increased α-hydroxybutyrate dehydrogenase is mainly seen in myocardial infarction. In addition, pernicious anemia, hemolytic anemia, renal infarction, myocardial injury, cardiomyopathy, malnutrition, folic acid and vitamin B12 deficiency, α-hydroxybutyrate dehydrogenase Acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759G01N2021/775
Inventor 朱世成何翠云陈飞王继华
Owner GUANGZHOU WONDFO BIOTECH
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