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1a-type erysipelothrix rhusiopathiae and application thereof

A technology of swine erysipelas and toxins, applied in the direction of bacteria, antibacterial drugs, veterinary vaccines, etc., to achieve strong pathogenicity, good immunogenicity, and good protective effect

Active Publication Date: 2019-04-12
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems existing in the treatment of swine erysipelas disease at present, the present invention provides a type 1a strain of erysipelas bacteria and its application

Method used

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  • 1a-type erysipelothrix rhusiopathiae and application thereof
  • 1a-type erysipelothrix rhusiopathiae and application thereof
  • 1a-type erysipelothrix rhusiopathiae and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: Isolation and identification of strain HN1573

[0024] 1.1 Preparation of medium

[0025] TSB liquid medium: Dissolve 30g of TSB broth powder (Tryptic Soy broth, tryptone soybean broth) in 1000mL of ultrapure water, sterilize at 115°C for 15min, add 50mL of fetal bovine serum;

[0026] TSA solid medium: Dissolve 40 g of TSA agar powder (Tryptic Soy Agar, tryptone soybean agar) in 1000 mL of ultrapure water, sterilize at 115 °C for 15 min, add 50 mL of fetal bovine serum; store at 4 °C for later use.

[0027] 1.2 Isolation and cultivation of strains

[0028] Aseptically collected heart blood from diseased pigs was inoculated on TSA solid medium, and cultured in a constant temperature incubator at 37°C for 24-36 hours, and the colonies with uniform needlepoint size, colorless and transparent, round, smooth and with neat edges grew out (see figure 1 ). After purification and inoculation, carry out Gram staining, the bacteria are Gram-positive bacteria, straig...

Embodiment 2

[0052] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0053] 2.1 Preparation of vaccine

[0054] Inoculate the Erysipelothrix suis strain HN1573 into the TSB liquid medium, culture it in a shaker at 37°C, collect the culture after 18 hours, measure the concentration, and adjust the number of bacteria in the culture to 2×10 9 CFU / mL, add 2mL formaldehyde solution per 1000mL bacterial solution, inactivate at 37°C for 12h. Add aluminum hydroxide adjuvant to the inactivated bacterial solution at a volume ratio of 9:1, and mix to prepare the inactivated Erysipelothrix suis inactivated vaccine.

[0055] 2.2 Vaccine Safety Test

[0056] Ten 30-day-old healthy nursery pigs were selected and divided into 2 groups with 5 pigs in each group.

[0057] Vaccine group: inject 4mL of this vaccine subcutaneously in the neck;

[0058] Control group: 4 mL of sterilized normal saline was injected subcutaneously into the neck. The body temperature of the animals was meas...

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Abstract

The invention discloses 1a-type erysipelothrix rhusiopathiae and an application thereof. The preservation number of the erysipelothrix rhusiopathiae is CGMCC NO:16808, names as erysipelothrix rhusiopathiae HN1573. The strain is separated from the heart blood of a fattening pig dying of acute sepsis. No other bacterium but the erysipelothrix rhusiopathiae grows during the separation on a TSA solidmedium when the proliferation titer in a TSB liquid medium is as high as 109 CFU / mL or above. A vaccine prepared by the strain HN1573 screened by the invention is simple in preparation process, and has good preventive effect on pig erysipelas disease.

Description

Technical field: [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a strain of Erysipelothrix suis and its application. Background technique: [0002] Erysipelothrix rhusiopathiae is a small, elongated, straight rod-shaped, Gram-positive bacterium that causes erysipelas in pigs and many other animals. Swine erysipelas usually presents with acute or subacute sepsis and chronic proliferative lesions. The heart is the primary target organ and usually presents with overt injury. Erysipelas is a disease that occurs worldwide and is of economic importance in Europe, Asia, Australia and the Americas. Erysipelothrix suis is also a zoonotic pathogen in humans and is the cause of skin diseases-like erysipelas. Live attenuated vaccines and bacteria have long been used to control swine erysipelas. However, the failure of attenuated vaccines often occurs, often causing severe reactions or death after injection. The hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCA61K39/0208A61K2039/521A61K2039/552A61P31/04C12N1/20C12N1/205C12R2001/01
Inventor 徐引弟王治方张青娴朱文豪许峰李海利郎利敏焦文强张立宪游一王克领
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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