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Method for promoting chlorella growth and oil accumulation by carrying out co-culture of alga and bacterium

A technology of oil accumulation and chlorella, which is applied in the field of bioenergy and microalgae oil, can solve the problems such as the influence of algae cell growth, achieve efficient oil production and solve contradictions

Active Publication Date: 2019-04-12
CHINA UNIV OF PETROLEUM (EAST CHINA)
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Problems solved by technology

Nitrogen-deficiency stress culture is currently the most effective and common way to induce oil accumulation in microalgae, but nitrogen is essential for the growth of algal cells, and the growth of algal cells will be greatly affected in a nitrogen-deficient environment, which causes Contradiction between "Nitrogen Deficiency Oil Production" and "Nitrogen Requirement for Growth"

Method used

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  • Method for promoting chlorella growth and oil accumulation by carrying out co-culture of alga and bacterium
  • Method for promoting chlorella growth and oil accumulation by carrying out co-culture of alga and bacterium
  • Method for promoting chlorella growth and oil accumulation by carrying out co-culture of alga and bacterium

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Embodiment

[0021] 1. Cultivation of Chlorella

[0022] A. Selection of Chlorella: The algae species were purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences (FACHB);

[0023] B. Purification of Chlorella: Sterile Chlorella was obtained by screening on BG11 plates (formula shown in Table 1) containing antibiotics. First, spread 50 μL of chlorella liquid evenly on the BG11 solid plate containing 100 mg / L ampicillin, 50 mg / L spectinomycin and 50 mg / L kanamycin sulfate, and put the plate into a light incubator for cultivation. After 7 days, single algal colony of Chlorella was picked, streaked onto a fresh BG11 plate containing antibiotics, repeated three times and identified by microscope to obtain sterile Chlorella;

[0024] C. Cultivation of Chlorella: BG11 liquid medium is used for cultivation, the cultivation temperature is 25±1°C, the light cycle is 14h:10h, and the light intensity is 9600lx.

[0025] Table 1 BG11 medium formula

[0026]

[0027] (...

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Abstract

The invention discloses a method for promoting chlorella growth and oil accumulation by carrying out co-culture of an alga and a bacterium. The method for promoting chlorella growth and oil accumulation by carrying out co-culture of the algae and the bacterium specifically comprise a step of co-culturing chlorella with Mesorhizobium sp. (a nitrogen-fixing bacterium) in certain proportion, so that,oxygen and extracellular metabolites released by the chlorella during growth process can be consumed by the Mesorhizobium sp. while carbon dioxide is metabolized by the Mesorhizobium sp. for photosynthesis of the chlorella cells; moreover, growth stimulators, including vitamins, glycopeptides and the like, are also released so as to promote growth of the chlorella. In addition, nitrogen-fixing capability of the Mesorhizobium sp. is further utilized so as to realize limited nitrogen supply; and thus, normal growth of the chlorella is ensured with efficient oil production of the chlorella cellsrealized.

Description

technical field [0001] The invention relates to the technical field of bioenergy and microalgae oil, in particular to a method for co-cultivating algal bacteria to promote the growth of chlorella and the accumulation of oil. Background technique [0002] Energy and the environment are important areas of common concern. With the extensive use of fossil fuels, the energy crisis and the greenhouse effect have become increasingly prominent, so the development of renewable and non-polluting new energy sources has attracted more and more attention. Bioenergy, also known as green energy, refers to the energy obtained from biomass raw materials such as straw, soybean, corn and microalgae, mainly including biogas, biohydrogen and biodiesel. The development of clean and efficient bioenergy technology is of great significance for improving the energy consumption structure and solving prominent problems of energy and environment. [0003] At present, bio-energy has been developed to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12P7/64C12R1/89C12R1/01
CPCC12N1/12C12N1/20C12P7/64
Inventor 葛保胜卫治金王皓楠刘恒恒郗丽君黄方邹建昊徐家乐
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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