Daptomycin fermentation culture medium and preparation method thereof

A fermentation medium and technology of daptomycin, applied in the field of daptomycin fermentation medium and its preparation, can solve the problems of high raw material cost and low yield of Streptomyces roseospora, and achieve low price, convenient transportation and storage , the effect of chemical stability

Active Publication Date: 2019-04-09
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the current situation that the cost of raw materials for culturing Streptomyces roseospora in the current fermentation medium is too high and the yield is not high, and to provide a method that can reduce the cost of daptomycin fermentation medium and increase the yield of daptomycin of fermentation medium

Method used

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  • Daptomycin fermentation culture medium and preparation method thereof
  • Daptomycin fermentation culture medium and preparation method thereof
  • Daptomycin fermentation culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The impact of embodiment 1 maltodextrin dosage on daptomycin output

[0056] The carbon sources in the fermentation medium are maltodextrin and glucose, the dosages are 7.2g / 100mL and 1.8g / 100mL respectively, and the remaining components are durian yeast powder 1.65g / 100mL, ferrous ammonium sulfate 0.086g / 100mL, Adjust to 7.5. The medium components are shown in the table below:

[0057]

[0058] According to the formulas of the above-mentioned fermentation media, the shake flasks were fermented for 5 days, and the fermentation units were measured by high performance liquid phase. According to the above experimental results, when the dosages of maltodextrin and glucose are 7.2g / 100mL and 1.8g / 100mL respectively, the daptomycin fermentation unit can maintain the same level as the existing fermentation unit and can reduce the production cost.

Embodiment 2

[0064] The impact of embodiment 2 carbon source type on daptomycin output

[0065] The carbon source in the fermentation medium is 7.2g / 100mL maltodextrin, the remaining components are Durian yeast powder 1.65g / 100mL, ferrous ammonium sulfate 0.086g / 100mL, and the pH is adjusted to 7.5. Add 1.8g / 100mL of another carbon source on the basis of maltodextrin, and the components of each medium are shown in the table below:

[0066]

[0067]

[0068] According to the formulas of the above-mentioned fermentation media, the shake flasks were fermented for 5 days, and the fermentation units were measured by high performance liquid phase. According to the above experimental results, on the basis of maltodextrin, molasses or brown sugar can also be added as a carbon source to reduce costs and further increase production. When molasses is added, the fermentation unit of daptomycin is the highest.

[0069] The impact of comparative example 2 sucrose on daptomycin yield

[0070] The...

Embodiment 3

[0073] The influence of embodiment 3 carbon source ratio on daptomycin output

[0074] The amount of carbon source in the fermentation medium is 9g / 100mL, the carbon source types are maltodextrin and molasses, the remaining components are durian yeast powder 1.65g / 100mL, ferrous ammonium sulfate 0.086g / 100mL, and the pH is adjusted to 7.5. Change the ratio of maltodextrin and molasses, and the components of each medium are shown in the table below:

[0075]

[0076] According to the formulas of the above-mentioned fermentation media, the shake flasks were fermented for 5 days, and the fermentation units were measured by high performance liquid phase. According to the above experimental results, on the basis of cost control, when the amount of maltodextrin is 7.5g / 100mL and the amount of molasses is 1.5g / 100mL, the fermentation unit of daptomycin is the highest.

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Abstract

The invention discloses a daptomycin fermentation culture medium and a preparation method thereof. The fermentation culture medium comprises a 7.2-11g/100mL carbon source , a 1.65-4.0 g/100mL nitrogensource, 0.086-0.5g/100mL inorganic salt and 0-0.1g/100mL trace elements. The carbon source comprises maltodextrin and a carbon source A, and the dosage of maltodextrin is 7.2-9g/100mL. The carbon source A is molasses or brown sugar and the dosage of the carbon source A is 0-1.8g/100mL. The nitrogen source is one or more of pomegranatep flower yeast powder, soybean powder, bean pulp, peanut flourand feather powder. The fermentation culture medium for culturing streptomyces roseosporus can improve the yield of daptomycin and lowers the production cost greatly, and has a very good industrial application prospect.

Description

technical field [0001] The invention relates to a daptomycin fermentation medium and a preparation method thereof. Background technique [0002] Daptomycin is a member of the lipopeptide family, which belongs to the ring ten lipopeptide structure group, and is fermented by Streptomyces roseosporus. It is currently the world's first successfully developed ring lipopeptide antibiotic. Daptomycin can destroy the function of bacterial cell membrane in many aspects and kill Gram-positive bacteria. Its characteristics are rapid inhibitory effect, low concentration required for in vivo and in vitro bactericidal effect, and long effective time of antibiotic action. [0003] Yu Guanghai et al. optimized the daptomycin basal fermentation medium by response surface methodology. The components of the original basal fermentation medium included glucose 0.75g / 100mL, dextrin 1.0g / 100mL, soluble starch 1.0g / 100mL, peptone 0.85g / 100mL, yeast extract powder 0.6g / 100mL, casein 0.85g / 100mL, L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12N1/20C12R1/465
CPCC12N1/20C12P21/02C07K7/08
Inventor 李继安林惠敏徐鲁何强卢雪欢邓旭张建斌李亚军
Owner SHANGHAI INST OF PHARMA IND
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