A kind of extracting liquid and its application in preserving tissue or cell, extracting rna

A technology of extraction solution and molar concentration, applied in the field of extraction solution, can solve problems such as low efficiency and incompatibility, and achieve the effects of high purity, stable properties and high yield

Active Publication Date: 2020-10-09
CHENGDU DAOSHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of low efficiency caused by the incompatibility between the existing solution preservation and the existing extraction solution in the prior art, the present invention provides an extraction solution and the extraction solution when preserving tissues or cells, extracting Applications in RNA

Method used

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  • A kind of extracting liquid and its application in preserving tissue or cell, extracting rna
  • A kind of extracting liquid and its application in preserving tissue or cell, extracting rna

Examples

Experimental program
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Embodiment 1

[0061] This embodiment provides an extraction solution, including 1.6 mol / L guanidine thiocyanate and 1 mol / L guanidine hydrochloride, and the pH of the extraction solution is adjusted to 4 by acetic acid. The extract is applied to the preserved tissue or cells.

Embodiment 2

[0063] The present embodiment provides an extract, comprising:

[0064]

[0065] Wherein, the pH of the extract is adjusted to 4 by acetic acid. This extract solution was used to extract RNA.

Embodiment 3

[0067] This embodiment provides an extract, including extract A and extract B;

[0068] Wherein extract A comprises the guanidine thiocyanate of 1.6mol / L and the guanidine hydrochloride of 1mol / L;

[0069] The extract B includes 57vt% water-saturated phenol, 0.24mol / L guanidine thiocyanate, 0.6mol / L ammonium thiocyanate, 11.25vt% glycerin and 0.01mol / L SDS; the balance is solvent.

[0070] The pH of the extract A and the extract B are both 4.

[0071] The amount of RNA extracted by using the extract solution in this example is 1000-1800 μg / mL, the measured A260 / A280 is in the range of 1.6-1.9, and the A260 / A230 is in the range of 1.6-1.9. Agarose gel electrophoresis pictures such as figure 1 shown.

[0072] Adopt above-mentioned extraction solution A and extraction solution B to carry out RNA extraction method, concrete steps are as follows:

[0073] 1. Tissues or cells preserved with extract A, said extract A includes 1.2-2.0 mol / L guanidine thiocyanate and 0.5-1.5 mol / L ...

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PUM

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Abstract

Disclosed is an extraction liquid, which comprises an extraction liquid A and an extraction liquid B; the extraction liquid A is applied in the preservation of tissue or cells, and the extraction liquid A comprises 1.2-2.0 mol / L of guanidinium thiocyanate and 0.5-1.5 mol / L of guanidine hydrochloride; the extraction liquid B comprises 30-65vt% of water-saturated phenol and 9-15vt% of glycerin by volume fraction, the remainder being a solvent; the extraction liquid B further comprises 0.20-1 mol / L of guanidine thiocyanate, 0.4-0.8 mol / L of ammonium thiocyanate and 0.0073-0.018 mol / L of surfactant by molar concentration; and the pH of both the extraction liquid A and the extraction liquid B is 3.5-4.5. The extraction liquid B is applied in the extraction of RNA.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to an extract and the application of the extract in preserving tissues or cells and extracting RNA. Background technique [0002] In modern molecular biology experiments and clinical molecular diagnosis, it is often necessary to isolate and purify RNA from tissues and cells, and the quality of RNA often affects the success or failure of molecular biology experiments such as cDNA library, RT-PCR and Northern Blot. [0003] The commonly used method is to first protect the tissue with a tissue protection solution, and then use the extraction solution to extract the RNA in the tissue or cells. Commonly used protection methods include liquid nitrogen protection and protection solution protection, and the commonly used protection solutions include RNA later reagents and RNA safety reagents. [0004] Among them, the liquid nitrogen protection method not only re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 赵伟廖东升赵仲玖邱坤
Owner CHENGDU DAOSHENG BIOTECH CO LTD
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