Method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology

A cation exchange, adenosine technology, applied in the field of biochemical industry, can solve the problems of heavy pollution, uneconomical, complex production route, etc., and achieve the effect of reducing production cost and improving production efficiency

Active Publication Date: 2019-04-05
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Route 1, the preparation method disclosed in Chinese patent document CN 103923083A, uses adenosine as a substrate to obtain acetyl adenine and tetraacetyl ribose after acetylation with acetic anhydride, and acetyl adenine is further hydrolyzed to obtain adenine, and theoretically four Acetyl ribose can be deacylated to generate D-ribose. Although this process route can obtain adenine and tetraacetyl ribose with a higher yield, the process is complicated, the amount of equipment used in production is large, and the quality of D-ribose obtained is very poor, and Produces more industrial three wastes
[0006] Line 2, the preparation method disclosed in the Chinese patent document CN101125854A, uses high-temperature liquid water to hydrolyze adenosine to prepare adenine and D-ribose, but the defect of this method is that the hydrolysis of adenosine needs to be carried out at high temperature, and the generated by-product D- Ribose is easily denatured at high temperature and cannot be used, which is very uneconomical
The preparation method disclosed in the Chinese patent document CN 105802938A uses adenosine hydrolase to hydrolyze adenosine to prepare adenine and D-ribose, but this method involves the preparation process of adenosine hydrolase and the subsequent separation of D- The extraction of ribose needs to remove protein, purine and unreacted adenosine. The process is quite cumbersome, resulting in complex production routes, high cost and heavy pollution.

Method used

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  • Method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology
  • Method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology
  • Method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology

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Experimental program
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Effect test

Embodiment 1

[0020] Step S1: First put 450g of demineralized water into the reactor, start stirring at the same time, then put 25g of adenosine into the reactor, raise the temperature to 60°C through the jacket, then add 80g of regenerated cation exchange resin and keep it for 2 hours Make adenosine absorbed by the cation exchange resin, then adjust the temperature of the reaction system to 90-95°C, keep the reaction at 60°C for 12 hours after the solution gradually dissolves, then stop heating, and cool down to room temperature through circulating water, and the reaction The liquid is filtered through the filter tank, and the cation exchange resin adsorbed with adenine and the jellyfish solution containing D-ribose are obtained after filtration;

[0021] Step S2: Soak the cation exchange resin obtained by filtering step S1 with a molar concentration of 1mol / L sodium hydroxide solution (wherein the amount of sodium hydroxide is 22g), then filter the filtrate containing adenine obtained by t...

Embodiment 2

[0024] Step S1: First put 500g of demineralized water into the reactor, start stirring at the same time, then put 28g of adenosine into the reactor, raise the temperature to 60°C through the jacket, then add 90g of regenerated cation exchange resin, and keep it for 2 hours Make adenosine absorbed by the cation exchange resin, then adjust the temperature of the reaction system to 90-95°C, keep the reaction at 60°C for 12 hours after the solution gradually dissolves, then stop heating, and cool down to room temperature through circulating water, and the reaction The liquid is filtered through the filter tank, and the cation exchange resin adsorbed with adenine and the jellyfish solution containing D-ribose are obtained after filtration;

[0025] Step S2: Soak the cation exchange resin obtained by filtering step S1 with a molar concentration of 1mol / L sodium hydroxide solution (wherein the amount of sodium hydroxide is 25g), then filter the filtrate containing adenine obtained by ...

Embodiment 3

[0028] Step S1: First put 550g of demineralized water into the reactor, start stirring at the same time, then put 30g of adenosine into the reactor, raise the temperature to 60°C through the jacket, then add 85g of regenerated cation exchange resin and keep it for 2 hours Make adenosine absorbed by the cation exchange resin, then adjust the temperature of the reaction system to 90-95°C, keep the reaction at 60°C for 12 hours after the solution gradually dissolves, then stop heating, and cool down to room temperature through circulating water, and the reaction The liquid is filtered through the filter tank, and the cation exchange resin adsorbed with adenine and the jellyfish solution containing D-ribose are obtained after filtration;

[0029]Step S2: Soak the cation exchange resin obtained by filtering step S1 with a molar concentration of 1mol / L sodium hydroxide solution (wherein the amount of sodium hydroxide is 28g), then filter the filtrate containing adenine obtained by th...

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Abstract

The invention discloses a method for hydrolyzing adenosine through cation exchange resin catalysis-separation coupled technology and belongs to the technical field of biochemical engineering. According to the method for hydrolyzing the adenosine through the cation exchange resin catalysis-separation coupled technology, catalysis-absorption coupled interaction of cation exchange resin is applied tocutting off carbon-hydrogen bonds in the molecular structure of adenosine and then efficiently hydrolyzing the adenosine to obtain adenine and meanwhile purify mother liquor containing D-ribose, thereby facilitating crystallization of D-ribose. The method for hydrolyzing the adenosine through the cation exchange resin catalysis-separation coupled technology can effectively reduce the production cost of the adenine and the D-ribose and improve the production efficiency and is applicable to scale production.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and specifically relates to a method for preparing adenine and D-ribose by hydrolyzing adenine nucleoside by using cation exchange resin catalysis-separation coupling technology. Background technique [0002] Adenine, also known as 6-aminopurine, is an important pharmaceutical raw material and intermediate. It can be used to produce anti-hepatitis B drugs adefovir dipivoxil, tenofovir dipivoxil, plant cell growth hormone 6-benzylaminopurine, vitamin B4, etc. Because adenine has such a wide range of uses, the amount used in the world has been continuously expanding in recent years. Therefore, the development of a green, environmentally friendly and economical method for the industrialization of adenine has aroused the interest of chemical and biological workers. D-ribose is an important five-carbon monosaccharide. It is an important component of ribonucleic acid (RNA), the genetic ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D473/34C07H3/02C07H1/00
CPCC07B2200/13C07D473/34C07H1/00C07H3/02
Inventor 刘学良吴宏伟陈明
Owner XINXIANG MEDICAL UNIV
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