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Human placenta tissue fluid injection and preparation method thereof

A technology of placental tissue fluid and placental tissue, which is applied in the direction of antiviral agents, drug combinations, and pharmaceutical formulas, etc.

Inactive Publication Date: 2019-04-05
江西润泽药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of human placenta tissue fluid injection and preparation method thereof, this method can effectively extract the active polypeptide in the placenta tissue fluid, and reduce toxin, bacterial growth, improve the content of active substance in the human placenta tissue fluid and the concentration in the preparation is Issues that need to be resolved

Method used

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Examples

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Comparison scheme
Effect test

preparation example Construction

[0011] A preparation method of human placenta tissue fluid injection, including the following steps:

[0012] (1) Select a healthy placenta, clean it to remove blood and dirt, and rinse it in sodium chloride solution to obtain pretreated placenta tissue;

[0013] (2) The pretreated placental tissue is crushed once, then freeze-dried by vacuum freeze-drying technology, and then crushed again to obtain dry placental tissue powder;

[0014] (3) Mix the dry placental tissue powder with water for injection, then add hydrochloric acid solution, adjust the pH of the mixed solution to 2.6-3.6, to obtain an acid mixture;

[0015] (4) Heat the acid mixture to hydrolyze, and then centrifuge to obtain acid hydrolyzed placental tissue fluid;

[0016] (5) Pass the acid-hydrolyzed placental tissue fluid into the protein adsorption column for adsorption and elution to obtain purified placental tissue fluid;

[0017] (6) Adjust the pH of the purified placental tissue fluid to 6.7-7.3 with sodium hydroxid...

Embodiment 1

[0033] A preparation method of human placenta tissue fluid injection, including the following steps:

[0034] (1) Collect the placenta of healthy parturients during full-term delivery. After testing for hepatitis B, hepatitis C, AIDS, syphilis and other viruses, select the placenta with negative virus test results as raw materials, and use them as raw materials to clean and remove blood and dirt. Rinse in the process to obtain pretreated placental tissue;

[0035] (2) The pre-treated placental tissue is crushed once, and then freeze-dried by vacuum freeze-drying technology. The conditions of the freeze-drying process include: putting the crushed placental tissue into the vacuum freeze dryer, closing the sealed door, and turning on the vacuum pump switch And the switch of the freeze dryer, pump the vacuum to 18kPa, and start the refrigeration at the same time. The temperature of the cold trap of the vacuum freeze dryer is reduced to -45℃ within 2 hours, and the holding time is 5 hou...

Embodiment 2

[0042] A preparation method of human placenta tissue fluid injection, including the following steps:

[0043] (1) Collect the placenta of healthy parturients during full-term delivery. After testing for hepatitis B, hepatitis C, AIDS, syphilis and other viruses, select the placenta with negative virus test results as raw materials, and use them as raw materials to clean and remove blood and dirt. Rinse in the process to obtain pretreated placental tissue;

[0044] (2) The pre-treated placental tissue is crushed once, and then freeze-dried by vacuum freeze-drying technology. The conditions of the freeze-drying process include: putting the crushed placental tissue into the vacuum freeze dryer, closing the sealed door, and turning on the vacuum pump switch And the freeze dryer switch, vacuum to 28kPa, and at the same time start the refrigeration, the cold trap temperature of the vacuum freeze dryer is reduced to -60 ℃ within 4 hours, and the holding time is 2 hours; the product is slo...

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Abstract

The invention discloses a human placenta tissue fluid injection and a preparation method of the human placenta tissue fluid injection. A placenta tissue is subjected to freeze-drying treatment and secondary pulverization to obtain placenta tissue dry powder, the problem of toxin and bacteria breeding caused by the fact that the placenta tissue is prone to deterioration in the conventional heatingand drying process is prevented, meanwhile, acid hydrolysis, centrifugal separation and the like are combined, and acid-hydrolyzed placenta tissue fluid is obtained. Compared with conventional repeated freezing, thawing, filtration and the like, the purifying efficiency is improved, further, adsorption and elution are carried out through a protein adsorption column, purer active peptide is obtained, and the purity of the active peptide is improved. The preparation method is simple in steps, easy to popularize in industry and high in application value. In addition, the protein adsorption columnis used for adsorption and elution, the content of active substances in the obtained human placenta tissue fluid is higher, use is more convenient in medical treatment, the human placenta tissue fluid can be subpackaged to prepare human placenta tissue fluid injections with different concentrations respectively, and use is more flexible and more convenient.

Description

Technical field [0001] The invention relates to human placental tissue fluid, in particular to a human placental tissue fluid injection and a preparation method thereof. Background technique [0002] Placental tissue fluid is an injection preparation that has medical, health, beauty, and health care functions after the placenta of a healthy mother has been inspected and quarantined, and the active ingredients in the placenta are extracted through special separation and extraction methods. Because the placenta contains more than 8,000 primitive life substances, and there are more than 270 human primitive enzymes that can be named at present, there are high requirements on the extraction process. The production technology includes multi-molecule separation and filtration technology, nano-purification technology, and carbon dioxide technology. Under the guarantee of the above technical conditions, the molecular weight of its core component can reach the molecular weight of small mol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K47/02A61K35/50A61P17/00A61P39/00A61P17/02
CPCA61K9/0019A61K9/08A61K35/50A61K47/02A61P17/00A61P17/02A61P39/00
Inventor 廖年生邹明明胡贤德眭荣春
Owner 江西润泽药业有限公司
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