Preparation method of probiotics additive applied for livestock breeding

A technology of probiotics and additives, which is applied in the field of preparation of probiotics additives, can solve the problems of payment and processing costs, cumbersome processing procedures, etc., and achieve the effects of reducing feed-to-meat ratio, improving breeding efficiency, and increasing quantity

Pending Publication Date: 2019-04-05
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Most of the fermented products of probiotic enterprises are obtained by expanding the seeds of the strains, fermenting, centrifuging and collecting the bacteria, and then freeze-drying or spray-drying to obtain bacterial powder. The supernatant after centrifugation is usually directly used as waste water. Discharge, the processing process is not only cumbersome, but also requires the enterprise to pay a certain processing fee for this

Method used

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  • Preparation method of probiotics additive applied for livestock breeding
  • Preparation method of probiotics additive applied for livestock breeding
  • Preparation method of probiotics additive applied for livestock breeding

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Collection of experimental example 1 fermentation supernatant

[0032] Although the fermentation methods of each probiotic are different, the subsequent treatment of the fermentation supernatant used in animal husbandry is not very different. Therefore, this example only takes Lactobacillus plantarum as an example to further illustrate, and provides a probiotic The method for fermentation and fermentation supernatant collection, concrete steps are as follows:

[0033] S1. Take a glycerol tube of Lactobacillus plantarum strain, inoculate it into MRS medium (a test tube with a liquid volume of 9ml), inoculate it at 35-37°C for 18-24 hours in anaerobic static culture, and name it system 1.

[0034] S2. Take system 1, inoculate it into MRS medium (200-400 ml) with an inoculum amount of 1-2%, at 35-37° C., anaerobic static culture for 14-18 hours, and name it system 2.

[0035] S3. Take system 2, inoculate it into MRS medium (10-20 L) with an inoculum amount of 1-2%, incuba...

experiment example 2

[0038] Experimental example 2 processing of fermentation supernatant

[0039] This embodiment uses the fermentation supernatant obtained in Example 1 to further provide a comparative test method for processing the fermentation supernatant and finally preparing it as a probiotic additive, as follows:

[0040] 1. Selection of pH value

[0041] Those skilled in the art should understand that microorganisms are not easy to grow under the condition of lower pH value. However, for different solutions, due to different components, the optimal pH value for inhibiting the growth of microorganisms is not the same. For this reason, in this embodiment, under the condition of different pH values, the fermentation supernatant is placed at room temperature for a certain period of time to detect the total number of mold yeast and bacteria in the solution. / ml (refer to GB 478915-2010 for the counting method, the same below), the total number of bacteria is 1×10 3 The number of days required...

experiment example 3

[0073] Application of Experimental Example 3 Probiotic Fermentation Supernatant

[0074] 1. Test animals

[0075] Experimental animals: According to the principles of the same genetic background, similar body weight, and same litter type, select "Du×Long×Da" three-way hybrid pigs with a body weight of about 16-18kg.

[0076] 2. Test materials

[0077] 1. Probiotic fermentation supernatant

[0078] The probiotic fermentation supernatant prepared by the methods of Examples 1 and 2 was selected for use.

[0079] 2. Test diet

[0080] The diet formula includes: corn 55.24%, barley 7%, expanded soybean 6.23%, dehulled soybean meal 20.68%, subflour 6%, cotton meal 4%, soybean oil 0.5%, salt 0.35%, and vitamins and trace elements .

[0081] In the diet of the control group, the diet and water were mixed and fed at a ratio of 1:2 to 4; in the diet of the test group, the diet and the fermentation supernatant of probiotics were also mixed at a ratio of 1:2 to 4 , Feeding.

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Abstract

The invention relates to the field of livestock breeding feed additives, in particular to a preparation method of a probiotics additive applied for livestock breeding. The method comprises the steps of conducing pH value adjustment on probiotics fermentation supernate, adding a stabilizer, adding an antioxidant, adding prebiotics and conducting sterilization treatment, wherein the probiotics fermentation supernate is a whole fermentation post-treatment solution which comprises fermentation supernatant of probiotics and the probiotics. According to the preparation method of the probiotics additive applied for livestock breeding, the probiotics additive applied for livestock breeding is stable in storage, has an obvious effect of promoting the growth performance of hogs when applied to feed,and is not only environmentally friendly, but also economical.

Description

technical field [0001] The invention relates to the field of animal husbandry feed additives, in particular to a preparation method of a probiotic additive applied to animal husbandry. Background technique [0002] As a kind of intestinal microorganisms, probiotics can regulate the balance of intestinal flora, inhibit harmful bacteria, improve host immunity, and promote digestion and absorption for the host. Its mechanism of action is mainly due to the beneficial regulatory effect of the metabolites produced during its growth, such as lactic acid, bacteriocin, and exopolysaccharides, on the host. [0003] Most of the fermented products of probiotic enterprises are obtained by expanding the seeds of the strains, fermenting, centrifuging and collecting the bacteria, and then freeze-drying or spray-drying to obtain bacterial powder. The supernatant after centrifugation is usually directly used as waste water. Discharge, the processing process is not only cumbersome, but also r...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/12A23K20/105A23K20/20A23K20/121A23K20/126A23K20/163A23K10/18
CPCA23K10/12A23K10/18A23K20/105A23K20/121A23K20/126A23K20/163A23K20/20A23K50/30A23V2400/133A23V2400/137A23V2400/145A23V2400/147A23V2400/143A23V2400/151A23V2400/125A23V2400/123A23V2400/113A23V2400/165A23V2400/181A23V2400/173A23V2400/175A23V2400/169A23V2400/513A23V2400/517A23V2400/519A23V2400/529A23V2400/533
Inventor 方曙光严涛朱建国陈珂可夏九学
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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