Reagent kit for constructing novel magnetic particle agglomeration cerebral ischemia animal model

An animal model and magnetic particle technology, applied in veterinary instruments, veterinary surgery, medical science, etc., can solve the problems of inconsistent thrombus simulation, large individual variation, and complicated operation, so as to improve the success rate of modeling and avoid direct losses. , the effect of short modeling time

Active Publication Date: 2019-04-02
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In clinical experiments, the effects of cerebral ischemia on the body are studied by establishing animal models of cerebral ischemia. The simulation of thrombus formation is not consistent with the actual situation. At the same time, it may cause direct damage to blood vessels, the mortality rate is high, the operation is complicated, the individual variation is large, and the operation is not reversible. Due to the possibility of multiple recurrences of transient cerebral ischemia, it cannot Establishment of a Sustainable Animal Model of Cerebral Ischemia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 A kit for constructing a novel magnetic particle aggregation animal model of cerebral ischemia, including a 10 mg / ml magnetic nanoparticle buffer solution, a soft linear magnetic strip, sterile paraffin, and sterile 1% heparin solution.

[0020] (1) Preparation of magnetic nanoparticle buffer solution:

[0021] Choose Fe, Co, Ni magnetic nanoparticles or ferroferric oxide magnetic nanoparticles or ferroferric oxide magnetic nanoparticles, preferably metal magnetic nanoparticles with a diameter of less than 150, the outer layer of the metal magnetic nanoparticles is coated with an inert The material can be polyethylene glycol, glucose or dextran, preferably polyethylene glycol with a molecular weight of 1000-2000, and the particle size of the metal magnetic nanoparticles coated with an inert material is 100-200nm; after sterilization , by mixing with physiological saline at a ratio of 5-20mg / ml, preferably 10mg / ml, the prepared buffer solution has uniform parti...

Embodiment 2

[0023] Example 2 A method of using a kit for constructing a novel magnetic particle aggregation animal model of cerebral ischemia 1. Experimental materials:

[0024] 1.1 Animal material

[0025] SD rats were selected, male, weaned rats (day 21 after birth), purchased from the Animal Center of Fudan University School of Medicine. After the animals were repurchased, they were randomly divided into groups and kept in separate cages. Maintain a quiet and comfortable environment during feeding, allowing them to drink water and eat freely. Two days before the start of the experiment, the experimenters touched and grasped the rats once a day in the morning and afternoon, and let the rats get used to the experimental equipment and equipment, in order to eliminate the stress stimulation caused by the experimental environment to the rats as much as possible and ensure the experimental results. accuracy.

[0026] Rats with a body weight of 230±30 g were selected when the rats were rai...

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PUM

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Abstract

The invention discloses a reagent kit for constructing a novel magnetic particle agglomeration cerebral ischemia animal model. The reagent kit comprises injection liquid for simulating thrombotic formation, wherein the injection liquid is a buffer solution containing magnetic nanometer particles, and when the magnetic nanometer particles are enriched in a special segment of blood vessels, thrombotic formation is simulated; and the reagent kit also comprises a guidance implant for guiding enriched magnetic nanometer particles. The guidance implant is used for wrapping soft threadlike magnetic stripes of the blood vessels. Through the reagent kit disclosed by the invention, the constructed cerebral ischemia animal model can highly cooperate with symptoms of cerebral ischemia, and has small damage to the animal model. The mortality rate for construction of the animal model is low.

Description

technical field [0001] The invention relates to the technical field of animal pharmacology experiments, and more specifically relates to a kit for constructing a novel magnetic particle aggregation cerebral ischemia animal model. Background technique [0002] Transient ischemic attack (TIA) is a transient insufficiency of blood supply in the carotid artery or vertebral-basilar artery system, causing focal cerebral ischemia and sudden, transient, reversible neurological dysfunction. The attack lasts for several minutes, and usually recovers completely within 30 minutes. After more than 2 hours, mild neurological deficits are often left, or CT and MRI show signs of brain tissue ischemia. TIA is more common in 34-65 years old, 25.3% of them are over 65 years old, more men than women. The onset is sudden, mostly in the case of body position change, hyperactivity, sudden rotation or flexion and extension of the neck. The onset is without aura, and there are transient neurologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 耿武军唐红丽
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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