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Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry

A technology of dexamethasone and LC/MS, applied in the field of medicine, can solve the problems of inability to achieve quantitative effect, muscle atrophy, growth inhibition and the like, and achieve the effects of simple pretreatment method, good peak shape and strong specificity

Inactive Publication Date: 2019-03-29
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dexamethasone and its residues have obvious toxic and side effects on the human body, and high doses of dexamethasone can cause side effects such as muscle atrophy and growth inhibition
At present, the methods used to detect the concentration of dexamethasone mainly use rapid kits or ultraviolet spectrophotometers, but the rapid kits can only perform a qualitative analysis and cannot achieve quantitative results. low degree

Method used

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  • Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry
  • Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry
  • Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Example: Human K 2 Determination of Dexamethasone Concentration in EDTA Plasma

[0030] 1. Experimental materials and analytical equipment

[0031] Dexamethasone (analyte): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0032] Dexamethasone-d5 (internal standard): Toronto Research Chemicals or equivalent or higher grade standard

[0033] The reagents used are shown in Table 1 below:

[0034] Table 1 Reagent Details

[0035] Reagent name

level

manufacturer

Acetonitrile

HPLC

J. T. Baker

formic acid

ACS

Adamas

Dipotassium phosphate

ACS

Adamas

methyl tert-butyl ether

HPLC

MREDA

[0036] Note: Reagents of the same grade or higher can also be used

[0037] The analytical equipment used is shown in Table 2 below:

[0038] Table 2 Details of equipment used

[0039] components

type

manufacturer

Binary pump (binary pump)

AC Pump

AB SCIEX ...

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Abstract

The invention discloses a method for determining concentration of dexamethason in plasma by liquid chromatography mass spectrometry. A liquid chromatography mass spectrometry system is used for determining. A sample to be determined is added with a certain amount of mixed organic solvent to extract twice, is separated by a chromatographic column after pretreatment, and is detected by a mass spectrometer. The method is rapid, accurate, high in sensitivity, and simple and convenient to operate. The method provides a basis for the blood drug concentration determination of dexamethason. The linearrange of a plasma standard curve in the method is 0.1 to 20 ng / mL. The intra-batch and inter-batch precision RSD are less than + / -15%. The method is suitable for determining the concentration of dexamethason in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining the concentration of dexamethasone in blood plasma by liquid mass spectrometry. Background technique [0002] Dexamethasone (Dexamethasone, DSMS) also known as dexamethasone, flumethasone, dexamethasone, is a synthetic adrenocortical hormone, is a glucocorticoid anti-inflammatory, anti-allergic drug, its pharmacological The main effects are anti-inflammatory, anti-toxic, anti-allergic, anti-rheumatic, and it is widely used clinically. Dexamethasone and its residues have obvious toxic and side effects on the human body, and high doses of dexamethasone can cause side effects such as muscle atrophy and growth inhibition. At present, the methods used to detect the concentration of dexamethasone mainly use rapid kits or ultraviolet spectrophotometers, but the rapid kits can only perform a qualitative analysis and cannot achieve quantitative results. lower ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 林明弘
Owner 徐州立顺康达医药科技有限公司
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