A method for detecting aflatoxin in corn feed stored in a feedlot

Abstract

A method for detecting aflatoxin in corn feed stored in a feedlot. The powder sample is obtained by processing the corn feed before use, and then the powder sample is subjected to continuous reflux extraction to obtain a corn feed extract. Finally, the corn feed extract is passed through an oxidation process. The micro-column chromatography tube composed of aluminum and silicon magnesium adsorbent is irradiated with ultraviolet light with a wavelength of 365nm to observe whether blue-violet fluorescence appears on the silicon magnesium adsorption layer, and use this as a basis to judge whether the batch of corn feed can be used. The present invention is based on the micro-column screening method. By inducing and urging the stored corn feed samples, the Aspergillus flavus that may be contained therein will multiply in large numbers, thereby producing a large amount of aflatoxin. It can be easily detected, avoiding the problem that the micro-column screening method cannot detect aflatoxin due to less mildew and low aflatoxin content, and improves the detection accuracy.

Description

Technical field[0001]The invention relates to the field of livestock product feed detection, in particular to a method for detecting aflatoxin in corn feed stored in a breeding farm.Background technique[0002]Corn is one of the main raw materials for animal feed, and large amounts of corn are often stored as feed in animal feed farms or animal feed production plants. Under normal circumstances, when these corn feeds are purchased, samples are usually sent to a special laboratory for testing to determine whether the aflatoxin is exceeded. However, during the storage process, due to storage time, storage conditions and changes in the external environment, some corn feeds may become moldy and produce aflatoxin. Since the storage period of corn feed is generally not too long, the number of moldy corn is very small, and the degree of mold is generally low. It is difficult to distinguish the presence of mold in corn feed from the surface.[0003]Even if this small amount of corn is moldy, it...

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Problems solved by technology

[0020] The detection cost of this method is low, but it also has the problems of cumbersome operation, time-consuming, unsatisfactory extraction and purification effects, and poor sensitivity. Aflatoxin, which will lead to the accumulation of trace amounts of aflatoxin in animals, thereby endangering the human body

[0021] Since a large amount of corn feed stored in a farm or animal feed factory (especially an animal feed farm) is used in large quantities every day, if it is tested every time before use, not only the detection cost is high (need to purchase professional equipment and Training professional inspectors), and more importantly, due to the need for frequent use, the frequency of detection is very high, the existing detection methods are very cumbersome, and it is difficult to detect trace amounts of mold, resulting in trace amounts of aflatoxin in animals enrichment, thereby affecting food and personal safety

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