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Method for controlling the pollution of Alicyclobacillus in water

A bacillus and alicyclic acid technology, applied in water/sewage treatment, chemical instruments and methods, light water/sewage treatment, etc., can solve the problems of increased cost, stimulation of spores, poor stability of retention effect, etc., to reduce the dosage , Improve the sterilization effect, the effect of low energy consumption

Inactive Publication Date: 2019-03-29
北京新时空科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical disinfectants are often used to kill spoilage microorganisms in the process of fruit and vegetable processing and cleaning, but disinfection by-products harmful to human health will be produced during the disinfection process, and for Alicyclobacillus with strong spore resistance, the inactivation effect not ideal
The commonly used sterilization methods in the existing food processing and production process are: 1. Membrane filtration, which requires frequent replacement of the filter membrane, resulting in increased costs and poor retention stability
2. High-temperature sterilization, because Alicyclobacillus is a heat-resistant bacterium, general high-temperature sterilization cannot kill it, and ultra-high temperature will affect the flavor of fruit juice
3. Pasteurization, conventional pasteurization is difficult to completely inactivate it, but may stimulate spores to germinate
Existing ultraviolet sterilization is generally irradiated by low-pressure mercury lamps. Due to the poor penetration of ultraviolet rays, it is usually used for the treatment of food factory workshops, equipment, surfaces of packaging materials, and processing water.

Method used

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  • Method for controlling the pollution of Alicyclobacillus in water
  • Method for controlling the pollution of Alicyclobacillus in water
  • Method for controlling the pollution of Alicyclobacillus in water

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1: Low-pressure UV irradiation alone

[0023] Add the spore suspension of Alicyclobacillus in a glass petri dish, utilize a 30W low-pressure mercury lamp to emit low-pressure ultraviolet light (254nm), and use a UV-B type ultraviolet radiation meter to measure the light intensity, and calculate according to the set irradiation dose corresponding exposure time. After reaching the estimated irradiation time, immediately add 0.1% sodium thiosulfate solution to terminate the reaction, and then take samples and count them. Draw a graph of the relationship between the inactivation rate and the irradiation dose, such as figure 1 shown in the curve.

Embodiment 2

[0024] Example 2: Medium pressure UV irradiation alone

[0025] Add the spore suspension of Alicyclobacillus into a glass petri dish, use a 3kW medium-pressure mercury lamp to emit medium-pressure ultraviolet rays, measure the light intensity with an ILT1700 ultraviolet radiation meter, and calculate the corresponding irradiation time according to the set irradiation dose . After reaching the estimated irradiation time, immediately add 0.1% sodium thiosulfate solution to terminate the reaction, and then take samples and count them. Draw a graph of the relationship between the inactivation rate and the irradiation dose, such as figure 2 shown in the curve.

Embodiment 3

[0026] Embodiment 3: separate hydrogen peroxide treatment

[0027] Add the spore suspension of Alicyclobacillus to the brown bottle, add different concentrations of hydrogen peroxide solution (the concentration of the hydrogen peroxide solution is very high, and the volume added is very small) and shake and mix immediately. After reacting in the dark at room temperature for a certain period of time, immediately add a 0.1% sodium thiosulfate solution to terminate the reaction, and then take samples for counting. Draw a graph of the relationship between the inactivation rate and the treatment time, such as image 3 shown in the curve.

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Abstract

The invention provides a method for controlling the pollution of Alicyclobacillus in water, which sterilizes and irradiates Alicyclobacillus in water with medium-pressure ultraviolet rays, thereby preventing the bacteria from entering subsequent production links from the source and causing cross-contamination among production equipment, furthermore thereby ensuring the quality of end products of fruit and vegetable industries. The medium-pressure ultraviolet light is combined with hydrogen peroxide, the method can properly reduce the irradiation dose of the medium-pressure ultraviolet ray on the premise of obtaining better sterilization effect, does not need to introduce new chemical substances in the sterilization process, and is safer to use in the food production and processing process,and has good application prospect especially in treating the processing and cleaning water of fruits and vegetables and other production water.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for controlling Alicyclobacillus pollution in fruit and vegetable processing and cleaning water by using medium-pressure ultraviolet rays. Background technique [0002] Juice spoilage incidents caused by Alicyclobacillus have occurred successively in many countries and regions in recent years, bringing huge economic losses to juice production enterprises, making this bacteria one of the most concerned pollutants in the juice industry. [0003] Alicyclobacillus spp. is acidophilic, heat-resistant, spore-forming, and stress-resistant, and its metabolites will affect the flavor of fruit juice at very low concentrations. The spores of the bacteria can contaminate semi-finished products or production materials in fruit juice production, including concentrated fruit juice and processing water, etc. It is difficult to detect when the pollution is low, so that the produc...

Claims

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Application Information

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IPC IPC(8): C02F1/32C02F1/72C02F103/32
CPCC02F1/32C02F1/722C02F2103/32C02F2305/023
Inventor 张灿白淼张明露田芳郭光
Owner 北京新时空科技股份有限公司
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