Method for improving content of soluble dietary fibers in Tibet Barley Aojiru
A technology of dietary fiber content and solubility, applied in the food field, can solve the problems of obtaining extraction rate, etc., and achieve the effect of preventing insulin secretion disorder, easy realization, and lowering cholesterol
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Embodiment 1
[0030] This embodiment relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder, the steps are as follows:
[0031] Step 1, crushing the highland barley seedlings after freeze-drying repeatedly, the time of each crushing process is 5-15s, the crushing speed is 15000-25000r / min, the interval time of each crushing process is 15-25s, after 45 mesh sieve;
[0032] Step 2, in the ultra-clean workbench, inoculate the Rhizopus oryzae preserved at 4°C on the PDA slant test tube culture medium, cultivate it at 28°C for 7 days, rinse the PDA slant with sterile water after it grows mature spores, and collect the spores. Properly dilute the spore concentration at 10 8 -10 9 spores / mL, inoculate the spore suspension into the seed medium at a ratio of 1:50 (v:v) (seed medium components: glucose 0.5g, peptone 0.05g, nutrient salt solution 5mL, trace element solution 0.05mL, spit Temperature-80 0.1mL, cooled after high-pressure steam steriliza...
Embodiment 2
[0039] This embodiment relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder, the steps are as follows:
[0040] Step 1, crushing the highland barley seedlings after freeze-drying repeatedly, the time of each crushing process is 5-15s, the crushing speed is 15000-25000r / min, the interval time of each crushing process is 15-25s, after 45 mesh sieve;
[0041] Step 2, in the ultra-clean workbench, inoculate the Rhizopus oryzae preserved at 4°C on the PDA slant test tube culture medium, cultivate it at 28°C for 7 days, rinse the PDA slant with sterile water after it grows mature spores, and collect the spores. Properly dilute the spore concentration at 10 8 -10 9 spores / mL, inoculate the spore suspension into the seed medium at a ratio of 1:50 (v:v) (seed medium components: glucose 0.5g, peptone 0.05g, nutrient salt solution 5mL, trace element solution 0.05mL, spit Temperature-80 0.1mL, cooled after high-pressure steam steriliza...
Embodiment 3
[0048] This embodiment relates to a method for increasing the content of soluble dietary fiber in highland barley grass powder, the steps are as follows:
[0049] Step 1, crushing the highland barley seedlings after freeze-drying repeatedly, the time of each crushing process is 5-15s, the crushing speed is 15000-25000r / min, the interval time of each crushing process is 15-25s, after 45 mesh sieve;
[0050] Step 2, in the ultra-clean workbench, inoculate the Rhizopus oryzae preserved at 4°C on the PDA slant test tube culture medium, cultivate it at 28°C for 7 days, rinse the PDA slant with sterile water after it grows mature spores, and collect the spores. Properly dilute the spore concentration at 10 8 -10 9 spores / mL, inoculate the spore suspension into the seed medium at a ratio of 1:50 (v:v) (seed medium components: glucose 0.5g, peptone 0.05g, nutrient salt solution 5mL, trace element solution 0.05mL, spit Temperature-80 0.1mL, cooled after high-pressure steam steriliza...
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