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Storage liquid of falling cells in human excrement sample

A technology for feces samples and exfoliated cells, which is applied in the preservation of human or animal bodies, the measurement/inspection of microorganisms, biochemical equipment and methods, etc. It can solve the problems of cell lysis and DNA extraction failure, and achieve simple proportioning and low cost. Low cost, effect of improving the quality of cell preservation

Inactive Publication Date: 2019-03-26
广州市达信智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few exfoliated cells in the feces. If the samples are not stored properly, the cells will be lysed and the final DNA extraction will fail.

Method used

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  • Storage liquid of falling cells in human excrement sample
  • Storage liquid of falling cells in human excrement sample
  • Storage liquid of falling cells in human excrement sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preservation solution for preserving cells in human feces is characterized in that 1L of the preservation solution contains 29.2g EDTA dry powder, 60.55g Tris dry powder, 0.58g NaCl dry powder, and the balance is sterilized water.

[0032] The preparation method of the exfoliated cell preservation solution in described a kind of human feces, comprises the following steps:

[0033] 1) Pour 29.2g of EDTA dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterilized water to 200ml to obtain a 0.5mol / L EDTA solution, and then adjust the pH to 8 with concentrated hydrochloric acid.

[0034] 2) Pour 60.55g of Tris dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterilized water to 500ml to obtain a 1mol / L Tris solution.

[0035] 3) Pour 2.92 Nacl dry powder into a beaker and dissolve it with a small amount of sterilized water. Afte...

Embodiment 2

[0039] The preservation solution for preserving cells in human feces is characterized in that 1L of the preservation solution contains 25g of EDTA dry powder, 65g of Tris dry powder, 0.2g of NaCl dry powder, and the balance is sterilized water.

[0040] The preparation method of the exfoliated cell preservation solution in described a kind of human feces, comprises the following steps:

[0041] 1) Pour 25g of EDTA dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterilized water to 200ml to obtain a 0.5mol / L EDTA solution, and then adjust the pH to 8 with concentrated hydrochloric acid.

[0042] 2) Pour 65g of Tris dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterilized water to 500ml to obtain a 1mol / L Tris solution.

[0043] 3) Pour 1.01Nacl dry powder into a beaker, dissolve it with a small amount of sterilized water, add steril...

Embodiment 3

[0047] The preservation solution for preserving cells in human feces is characterized in that 1L of the preservation solution contains 35g of EDTA dry powder, 55g of Tris dry powder, 0.8g of NaCl dry powder, and the balance is sterilized water.

[0048] The preparation method of the exfoliated cell preservation solution in described a kind of human feces, comprises the following steps:

[0049] 1) Pour 35g of EDTA dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterilized water to 200ml to obtain a 0.5mol / L EDTA solution, and then adjust the pH to 8 with concentrated hydrochloric acid.

[0050] 2) Pour 55g of Tris dry powder into a beaker and dissolve it with a small amount of sterilized water. After the dissolution is complete, add sterilized water to 500ml to obtain a 1mol / L Tris solution.

[0051] 3) Pour 4.02Nacl dry powder into a beaker and dissolve it with a small amount of sterilized water. After t...

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Abstract

The invention relates to storage liquid of falling cells in a human excrement sample. The storage liquid is used for storing the falling cells in the human excrement sample, and comprises main ingredients of EDTA, Tris and NaCl, wherein the EDTA is a stabilizing agent, so that the storage liquid can be put at room temperature; the Tris is a buffer agent, and can maintain the intracellular and extracellular osmotic pressure and the pH value; the NaCl can maintain the electrolyte balance and maintain the cell osmotic pressure. The storage liquid has the advantages that the mixture ratio is simple; the cost is low; the use is convenient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preservation solution of exfoliated cells in human feces samples. Background technique [0002] Colorectal cancer is one of the most common diseases today. About 1.2 million patients worldwide are diagnosed with colorectal cancer each year, and more than 600,000 patients die directly or indirectly from colorectal cancer. There are significant differences in its incidence in various regions, which is closely related to the so-called lifestyle. Men have a higher incidence of colorectal cancer than women. In addition, the incidence of colorectal cancer increases with age. For example, the median age of colorectal cancer in developed countries is 70 years old. [0003] Although genetic factors are risk factors for colorectal cancer, most colorectal cancers are sporadic and develop as adenoma-tumours over several years. The mainstays of current treatment for colorectal ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806A01N1/02
CPCA01N1/021C12Q1/6806
Inventor 李明田溯宁杨俊
Owner 广州市达信智能科技有限公司
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