Haplotype genetic marker for improving Sunite sheep prolificacy and application thereof

A Sunit sheep, genotyping technology, applied in the field of haplotype genetic markers and its application to improve the litter size of Sunit sheep, can solve the problem of low incidence

Pending Publication Date: 2019-03-22
INNER MONGOLIA UNIVERSITY +1
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Problems solved by technology

[0006] Guan Feng et al. used PCR-RFLP method to study GDF9 gene G8 mutation in Hu sheep, Charolais, Dorset, Suffolk, Chinese Merino meat multi-fetal lines, Chinese Merino sheep and Romney 7 sheep The distribution in the population, it was found that the G8 homozygous mutation was only detected in the Hu sheep population, but the incidence rate was extremely low (0.645%, 2 / 310)

Method used

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  • Haplotype genetic marker for improving Sunite sheep prolificacy and application thereof
  • Haplotype genetic marker for improving Sunite sheep prolificacy and application thereof
  • Haplotype genetic marker for improving Sunite sheep prolificacy and application thereof

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Embodiment 1

[0039] Example 1. Establishment of a method for detecting haplotypes that increase the litter size of Sunit sheep and its effect verification.

[0040] 1. Establishment of a method for detecting haplotypes that improve the fertility of Sunit sheep

[0041] 1) Preparation of template materials Blood samples of Sunite sheep were collected and the number of lambs was recorded. The collected Sunite sheep came from the Sunite Left Banner of Xilingol League, Inner Mongolia. The blood samples were stored in anticoagulant tubes for later use.

[0042] 2) PCR product sequencing.

[0043] Randomly select 20 Sunit sheep (10 double-lamb ewes + 10 single-lamb ewes) for PCR product sequencing ( figure 1 ), found the SNP sites located in the GDF9 promoter, namely -332, -534, -799 sites, and continued to expand the population (168 single lambs + 69 double lambs) ( figure 1 , figure 2 , image 3 ), linkage disequilibrium analysis was performed on these three loci ( Figure 4 ).

[0044]...

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Abstract

The invention provides a method for improving the Sunite sheep prolificacy by using GDF9 genes. The linkage of three sites -332, -534 and -779 is unbalanced. The method uses a PCR method for amplifying the Sunite sheep GDF9 gene GenBank Accession Number into 41771049 to 41771267 site nucleotide fragments (-534 site) of NC_019462; an amplification product is subjected to RFLP detection; if a striponly occurs in the 214bp position, the result proves that the genotype is CC; if the strips occur in 214bp, 129bp and 85bp positions, the result shows that the genotype is the heterozygous genotype CT; if the strips only occur in the 129 and 85bp positions, the result proves that the genotype is TT; the prolificacy of the Sunite sheep with the genotype of CT(-332 site), AG(-534 site) and CT(-779 site) is higher than that of the Sunite sheep with the genotype of CT(-332 site), AA(-534 site ) and CC(-779 site). The PCR-RFLP is used for fast, simply and conveniently detecting the haplotype genetic marker polymorphism; an accurate simple and convenient method is provided for improving the Sunite sheep prolificacy by using a molecular marker auxiliary breeding technology.

Description

technical field [0001] A haplotype genetic marker for increasing litter size of Sunit sheep and its application. Background technique [0002] GDF9 (growth differentiation factor 9, GDF9) is growth differentiation factor 9, and BMP15 gene belongs to transforming growth factor β (TGFβ) superfamily members. The GDF9 gene of sheep is located on chromosome 5, with a total length of about 2.5kb, which contains 2 exons and 1 intron. Exon 1 is 397bp long, encoding 134 amino acids, and exon 2 is 968bp long. It encodes 456 amino acids, and the intron is 1126bp long. (Sadighi M, Bodensteiner K J, Beattie A E et al. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheepchromosome 5. [J]. Animal Genetics, 2002,33(3):244-245.). [0003] Sonid sheep are produced in Sonid Left Banner and Sunid Right Banner of Xilin Gol League, Inner Mongolia Autonomous Region, known as Gobi sheep. Its main breeding method is natural grazing on pure natural pastures. The climate is char...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/124
Inventor 李光鹏佟彬王嘉鹏白春玲刘志刚吕菲菲
Owner INNER MONGOLIA UNIVERSITY
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