Method for rapidly detecting kernel rot resistance by corn dry seeds
A technology of corn dried and dried seeds is applied in the field of plant disease resistance, which can solve the problems of long cycle, large space and labor consumption, single pathogenic bacteria, etc., and achieve the effects of increasing water content, shortening cycle and prolonging fetal germination.
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Embodiment 1
[0024] A method for rapidly detecting grain rot resistance using dried corn seeds, said method comprising the following steps:
[0025] (1) Preparation before inoculation of corn kernels: select clean and healthy dry seeds of corn susceptible variety CML171, clean them, soak the dry seeds in acetic acid penicillin solution at 25°C for 6 hours, remove the tip of the base of the dry seeds, and expose the hilum. Store in a refrigerator at 4°C until inoculation;
[0026] Among them, the preparation method of acetic acid penicillin solution is: add 1mL of hot triethanolamine to 10mg of penicillin, stir to dissolve, add 114.3mL of acetic acid solution, dilute to 1L with distilled water, and make 2mol / L acetic acid concentration of 10mg / L solution of penicillin concentration.
[0027] (2) Preparation of grain rot spore suspension: select 10 corn ears with severe grain rot from the field, soak them in sterile water for 10 minutes; repeatedly wash them with distilled water for 3 time...
Embodiment 2
[0031] A method for rapidly detecting grain rot resistance using dried corn seeds, said method comprising the following steps:
[0032] (1) Preparation before inoculation of corn kernels: select clean and healthy dry seeds of corn susceptible variety CML171, clean them, soak the dry seeds in acetic acid penicillin solution at 26.5°C for 11 hours, remove the tip of the base of the dry seeds, and expose the hilum. Store in a refrigerator at 6°C until inoculation;
[0033] Among them, the preparation method of acetic acid penicillin solution is as follows: add 1mL of hot triethanolamine to 17.5mg of penicillin, stir to dissolve, add 285.8mL of acetic acid solution, distill the volume to 1L with distilled water, and make 8mol / L acetic acid concentration 17.5mg / L solution of penicillin concentration.
[0034] (2) Select 10 ears of corn with serious grain rot from the field, soak them in sterile water for 10 minutes; wash them with distilled water repeatedly for 3 times, and use 50...
Embodiment 3
[0038] A method for rapidly detecting grain rot resistance using dried corn seeds, said method comprising the following steps:
[0039] (1) Preparation before inoculation of corn kernels: select clean and healthy dry seeds of corn susceptible variety CML171, wash them, soak the dry seeds in acetic acid penicillin solution at 28°C for 16 hours, remove the tip of the base of the dry seeds, and expose the hilum. Store in a refrigerator at 8°C until inoculation;
[0040] Among them, the preparation method of acetic acid penicillin solution is as follows: add 1mL of hot triethanolamine to 25mg of penicillin, stir to dissolve, add 457.2mL of acetic acid solution, distill the volume to 1L with distilled water, and make 8mol / L acetic acid concentration of 25mg / L solution of penicillin concentration.
[0041] (2) Select 10 ears of corn with serious grain rot from the field, soak them in sterile water for 10 minutes; wash them with distilled water repeatedly for 3 times, and use 50 ml o...
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