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Method for rapidly detecting kernel rot resistance by corn dry seeds

A technology of corn dried and dried seeds is applied in the field of plant disease resistance, which can solve the problems of long cycle, large space and labor consumption, single pathogenic bacteria, etc., and achieve the effects of increasing water content, shortening cycle and prolonging fetal germination.

Inactive Publication Date: 2019-03-22
JINGXI XIUMEI BIANCHENG AGRI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenic bacteria of this detection method is single, the research is seasonal, the cycle is long, and it consumes a lot of space and manpower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for rapidly detecting grain rot resistance using dried corn seeds, said method comprising the following steps:

[0025] (1) Preparation before inoculation of corn kernels: select clean and healthy dry seeds of corn susceptible variety CML171, clean them, soak the dry seeds in acetic acid penicillin solution at 25°C for 6 hours, remove the tip of the base of the dry seeds, and expose the hilum. Store in a refrigerator at 4°C until inoculation;

[0026] Among them, the preparation method of acetic acid penicillin solution is: add 1mL of hot triethanolamine to 10mg of penicillin, stir to dissolve, add 114.3mL of acetic acid solution, dilute to 1L with distilled water, and make 2mol / L acetic acid concentration of 10mg / L solution of penicillin concentration.

[0027] (2) Preparation of grain rot spore suspension: select 10 corn ears with severe grain rot from the field, soak them in sterile water for 10 minutes; repeatedly wash them with distilled water for 3 time...

Embodiment 2

[0031] A method for rapidly detecting grain rot resistance using dried corn seeds, said method comprising the following steps:

[0032] (1) Preparation before inoculation of corn kernels: select clean and healthy dry seeds of corn susceptible variety CML171, clean them, soak the dry seeds in acetic acid penicillin solution at 26.5°C for 11 hours, remove the tip of the base of the dry seeds, and expose the hilum. Store in a refrigerator at 6°C until inoculation;

[0033] Among them, the preparation method of acetic acid penicillin solution is as follows: add 1mL of hot triethanolamine to 17.5mg of penicillin, stir to dissolve, add 285.8mL of acetic acid solution, distill the volume to 1L with distilled water, and make 8mol / L acetic acid concentration 17.5mg / L solution of penicillin concentration.

[0034] (2) Select 10 ears of corn with serious grain rot from the field, soak them in sterile water for 10 minutes; wash them with distilled water repeatedly for 3 times, and use 50...

Embodiment 3

[0038] A method for rapidly detecting grain rot resistance using dried corn seeds, said method comprising the following steps:

[0039] (1) Preparation before inoculation of corn kernels: select clean and healthy dry seeds of corn susceptible variety CML171, wash them, soak the dry seeds in acetic acid penicillin solution at 28°C for 16 hours, remove the tip of the base of the dry seeds, and expose the hilum. Store in a refrigerator at 8°C until inoculation;

[0040] Among them, the preparation method of acetic acid penicillin solution is as follows: add 1mL of hot triethanolamine to 25mg of penicillin, stir to dissolve, add 457.2mL of acetic acid solution, distill the volume to 1L with distilled water, and make 8mol / L acetic acid concentration of 25mg / L solution of penicillin concentration.

[0041] (2) Select 10 ears of corn with serious grain rot from the field, soak them in sterile water for 10 minutes; wash them with distilled water repeatedly for 3 times, and use 50 ml o...

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Abstract

The invention relates to the field of plant disease resistance, in particular to a method for rapidly detecting kernel rot resistance by corn dry seeds. The method comprises the following steps: (1) preparing before inoculating corn kernels; (2) preparing pathogenic spore suspension liquid; (3) inoculating kernels; and (4) performing moisturizing culture on the kernels. The method has the following advantages: the pathogenic bacteria for inoculation have many varieties; the inoculating materials are dry seeds and can be used while being taken; operation is simple, can be completed indoors andis not limited by seasons; few places are occupied; the efficiency is high; the repeatability is high; the corn kernel rot resistance detection time is effectively shortened; the kernel rot resistancecan be rapidly detected; and the application value is great.

Description

【Technical field】 [0001] The invention relates to the technical field of plant disease resistance, in particular to a method for rapidly detecting grain rot resistance by using dry corn seeds. 【Background technique】 [0002] Corn kernel rot is one of the important diseases in the later stage of corn growth, and it is a worldwide disease. It has the characteristics of rapid spread and serious damage, resulting in reduced corn production and reduced corn quality. It is a disease caused by a variety of fungal infections. It is caused by nearly 20 kinds of molds such as Fusarium graminearum, Fusarium clumps, Fusarium laminarum, Penicillium, Aspergillus, Cladosporium, and Monosporium. Diseases caused by compound infestation. [0003] Kernel rot damages corn ears and kernels, causing large pieces or entire ears to rot, and diseased kernels shrink, dull, and not full, resulting in reduced yields and affecting the germination rate of infected seeds and the survival rate of seedlin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/77C12R1/80C12R1/66C12R1/645
CPCC12Q1/18G01N2333/37G01N2333/38G01N2333/385
Inventor 谢宗宜
Owner JINGXI XIUMEI BIANCHENG AGRI SCI & TECH CO LTD
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