Fluorescence imaging system and fluorescence imaging method for quantitative detection of spatial distribution of photosensitizer

Abstract

The invention relates to a fluorescence imaging system for quantitative detection of spatial distribution of a photosensitizer. The fluorescence imaging system is characterized by comprising a first LED light source, a first freeform-surface total reflection lens, a first fly-eye lens, a first integrated lens, a first reflection mirror, a second LED light source, a second freeform-surface total reflection lens, a second fly-eye lens, a second integrated lens, a second reflection mirror, a first semi-transparent semi-reflective mirror, a second semi-transparent semi-reflective mirror, a laser light source, a beam expander, a third fly-eye lens, a digital micromirror device, a projection lens, a condensing lens, a CMOS (complementary metal oxide semiconductor) camera and a computer. The fluorescence imaging system has the advantages that diffuse reflection images are adopted to quantitatively calibrate the effect of tissue optical properties on fluorescence images, quantitative fluorescence imaging of the spatial distribution of the photosensitizer is realized, and the spatial distribution and variation conditions of the concentration of the photosensitizer in diseased tissue are obtained.

Description

Technical field [0001] The invention relates to the field of biomedical imaging, and relates to a fluorescence imaging system and method for quantitatively detecting the spatial distribution of a photosensitizer. Background technique [0002] The use of photosensitizers can form a relatively high concentration of accumulation in tumor tissues and can produce fluorescence after being irradiated with light of appropriate wavelengths. Fluorescence detection technology can be applied clinically. By analyzing the fluorescence intensity, not only the growth position of the diseased tissue and the lesion can be determined The boundary between tissue and normal tissue, as well as the treatment area, can also monitor the changes in photosensitizer concentration in real time during photodynamic therapy, and guide real-time dose adjustment, so as to realize fluorescence diagnosis, surgical guidance and treatment monitoring of tumors and other diseases. A wide range of clinical applications....

AI Technical Summary

Problems solved by technology

At present, fluorescence spectroscopy technology is usually used in clinical practice for fluorescence detection and combined with calibration algorithms to realize quantitative monitoring of photosensitizer concentration. The measurement method often produces contact pressure, changes the local optical characteristic parameters of the tissue, and affects the accuracy of the measurement; in addition, the spectral detection method can only obtain the information of a single point, and cannot reflect the concentration distribution of the photosensitizer in the target tissue with a large area

Fluorescence imaging technology can detect the concentration distribution of photosensitizers in a large target tissue by imaging. However, in the imaging mode, the light detected by each pixel on the imaging surface comes from the sampling signal in a certain volume of the tissue. The average value of ; the imaging surface may also receive photons from outside the target tissue, making the correction of fluorescence signals based on imaging techniques more difficult than that based on spectral techniques

Existing fluorescence imaging systems often use a semi-empirical ratio algorithm, that is, perform a ratio operation between the fluorescence image and the reflected light data. Such a fluorescence imaging system is not only complex in structure, expensive, but also unable to analyze the absorption and scattering characteristic parameters of the tissue separately. Can be applied to specific tissue samples

At the same time, when the excitation light with a uniform distribution of illumination intensity reaches a specific tissue depth, it can only excite the photosensitizer at this depth, and cannot correctly reflect the concentration distribution information of the photosensitizer at different depths around it.

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