A fluorescent imaging system and method for quantitatively detecting the spatial distribution of photosensitizers

Abstract

The invention relates to a fluorescence imaging system for quantitatively detecting the spatial distribution of photosensitizers, which is characterized by comprising a first LED light source, a first free-form surface total reflection lens, a first fly-eye lens, a first integrating lens, a first reflecting mirror, a first Two LED light sources, the second free-form surface total reflection lens, the second fly-eye lens, the second integrator lens, the second mirror, the first half mirror, the second half mirror, the laser light source, the beam expander, A third fly-eye lens, a digital micromirror, a projection lens, a condenser lens, a CMOS camera and a computer; the invention uses the diffuse reflection image to quantitatively correct the influence of the optical properties of the tissue on the fluorescent image, so as to realize quantitative fluorescent imaging of the spatial distribution of the photosensitizer, and obtain Concentration spatial distribution and changes of photosensitizers in diseased tissues.

Description

technical field [0001] The invention relates to the field of biomedical imaging, and relates to a fluorescent imaging system and method for quantitatively detecting the spatial distribution of photosensitizers. Background technique [0002] Utilizing the characteristics that photosensitizers can form a relatively high concentration in tumor tissue and produce fluorescence after being irradiated with light of an appropriate wavelength, fluorescence detection technology can be applied clinically. By analyzing the fluorescence intensity, not only can the growth location of the lesion tissue be determined, the lesion The boundary between tissue and normal tissue, as well as the treated area, can also monitor the change of photosensitizer concentration in real time during photodynamic therapy, and guide real-time dose adjustment, so as to realize fluorescence diagnosis, surgical guidance and treatment monitoring for tumors and other diseases. Wide range of clinical applications. ...

AI Technical Summary

Problems solved by technology

At present, fluorescence spectroscopy technology is usually used in clinical practice for fluorescence detection and combined with calibration algorithms to realize quantitative monitoring of photosensitizer concentration. The measurement method often produces contact pressure, changes the local optical characteristic parameters of the tissue, and affects the accuracy of the measurement; in addition, the spectral detection method can only obtain the information of a single point, and cannot reflect the concentration distribution of the photosensitizer in the target tissue with a large area

Fluorescence imaging technology can detect the concentration distribution of photosensitizers in a large target tissue by imaging. However, in the imaging mode, the light detected by each pixel on the imaging surface comes from the sampling signal in a certain volume of the tissue. The average value of ; the imaging surface may also receive photons from outside the target tissue, making the correction of fluorescence signals based on imaging techniques more difficult than that based on spectral techniques

Existing fluorescence imaging systems often use a semi-empirical ratio algorithm, that is, perform a ratio operation between the fluorescence image and the reflected light data. Such a fluorescence imaging system is not only complex in structure, expensive, but also unable to analyze the absorption and scattering characteristic parameters of the tissue separately. Can be applied to specific tissue samples

At the same time, when the excitation light with a uniform distribution of illumination intensity reaches a specific tissue depth, it can only excite the photosensitizer at this depth, and cannot correctly reflect the concentration distribution information of the photosensitizer at different depths around it.

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