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A rapid detection method for biguanide compounds in health products

A detection method and biguanide technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc. Time and other issues, to achieve the effect of low false detection and missed detection, rapid response, and avoidance of secondary pollution

Active Publication Date: 2020-10-09
北京倍肯恒业科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In order to overcome the shortcomings of the existing technology in the detection of biguanides in health products, such as high detection cost, long time-consuming, high quality requirements for detection personnel, interference caused by complicated sample matrices, and hidden dangers caused by the use of reagents, this paper The embodiment of the application provides a rapid detection method for biguanide compounds in health products, which can quickly detect biguanide components in health products on site, and can achieve quantitative detection, with extremely low false detection and missed detection rates, and the detection process Reduce reagent contamination problems caused by existing rapid tests on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] 1. Preparation of test paper:

[0050] (1) Take degradable test paper;

[0051] (2) Preparation of soaking solution: take sodium nitroprusside, potassium ferricyanide, polyethylene glycol and triton X-100 with a volume ratio of 10:7:2:1, and control the percentage concentration of each component as follows: 1%, 2.2%, 3%, 1%, stir and mix evenly;

[0052] (3) Soak the degradable test paper in the soaking solution for 15 minutes;

[0053] (4) Place the soaked biguanide detection test paper substrate in a desiccator at 60 degrees Celsius and dry for 30 minutes to obtain a biguanide detection test paper.

[0054] 2. Preparation of extract

[0055] Take anhydrous ethanol and 20 mol / L potassium hydroxide solution, the volume ratio is 300:1, stir and mix evenly to obtain.

[0056] 3. Preparation of test card:

[0057] Take the upper and lower covers corresponding to the upper and lower sides and made of PVC boards. There is a groove in the center of the upper surface of t...

Embodiment 1

[0058] Example 1: Rapid detection method for biguanide in soft capsule hypoglycemic health products

[0059] (1) Pretreatment of samples to be tested:

[0060] Weigh 0.2g sample into a 2.0mL centrifuge tube, add 1.0mL extract, centrifuge the 2.0mL centrifuge tube at 3000r / min for 1min, and take the supernatant directly into a 1.5mL centrifuge tube.

[0061] (2) Detection:

[0062] Take 200uL of the diluted solution in another 1.5mL centrifuge tube, add 130uL of the supernatant, and dilute the supernatant to obtain the test solution. Take 70uL of the test solution and add it dropwise to the middle of the sample hole of the test card, and react at room temperature 30min.

[0063] (3) Quantitative test:

[0064] Place the biguanide detection card on the handheld, and use the principle of reflectance spectroscopy to read the content of biguanide in health products.

Embodiment 2

[0065] Example 2: Rapid detection method for biguanides in hard capsules, teas, and tablets for hypoglycemic health care products

[0066] (1) Pretreatment of samples to be tested:

[0067] Weigh 0.2g sample into a 2.0mL centrifuge tube, add 1.0mL extract, centrifuge the 2.0mL centrifuge tube at 3000r / min for 1min, and take the supernatant directly into a 1.5mL centrifuge tube.

[0068] (2) Detection:

[0069] Take 200uL of the diluted solution in another 1.5mL centrifuge tube, add 100uL of the supernatant of the filtrate, and mix well to obtain the test solution. Take 70uL of the test solution and add it dropwise to the middle of the sample hole of the test card, and react at room temperature for 30 minutes.

[0070] (3) Quantitative test:

[0071] Place the biguanide detection card on the handheld, and use the principle of reflectance spectroscopy to read the content of biguanide in health products.

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Abstract

The invention provides a quick detection method for biguanide compounds in health products, belonging to the technical field of detection analysis of health products. The quick detection method comprises the following steps: preparing detection test paper, carrying out pretreatment on a to-be-tested sample, carrying out chromogenic reaction on the pretreated sample on the detection test paper, andif the test paper presents orange red, judging that the sample contains the biguanide compounds. According to the method, biguanide components in the health products can be rapidly detected on site,the quantitative detection can be realized, the false detection rate and the omission rate are extremely low, and the problem that a reagent is polluted during the rapid detection in the market in thedetection process is solved.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis of health products, and in particular relates to a rapid detection method for biguanide compounds in health products. Background technique [0002] Biguanide compounds are one of the elders of the hypoglycemic drug family, mainly including metformin hydrochloride, phenformin hydrochloride and buformin hydrochloride, and are effective first-line drugs for obese diabetic patients. Biguanide compounds are cheap, and combined with other chemical hypoglycemic drugs can enhance the hypoglycemic effect, so they appear frequently in illegal additions. However, when biguanide compounds are used as drugs for hypoglycemia, there are serious adverse reactions including lactic acidosis. An adverse reaction with a high mortality rate, especially for patients with severe heart, liver, lung, and kidney dysfunction or the elderly, it is not recommended to use. [0003] The trade names of metformin ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 姚世平刘光中高鲁嘉丁绍伟李心乐刘春秀李春玲
Owner 北京倍肯恒业科技发展股份有限公司
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