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A low-temperature straw-degrading fungus jgdw-1 and its agent and application

A technology of JGDW-1 and fungal agent, which is applied in the field of agricultural resources and environment, can solve the problems of long fermentation cycle, low temperature, slow cellulose degradation, etc., to accelerate the return of straw to the field for maturity, broad application prospects, and promote sustainable development Effect

Active Publication Date: 2020-09-08
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Medium- and high-temperature cellulose-degrading bacteria must work under certain temperature conditions, and there are certain restrictions on temperature, while low-temperature cellulose-degrading bacteria have much lower temperature requirements for life activities than medium- and high-temperature bacteria, and are especially suitable for solving winter problems in northern China. Long time, low temperature, slow cellulose degradation, and long fermentation cycle

Method used

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  • A low-temperature straw-degrading fungus jgdw-1 and its agent and application
  • A low-temperature straw-degrading fungus jgdw-1 and its agent and application
  • A low-temperature straw-degrading fungus jgdw-1 and its agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and Identification of Straw Degrading Fungal Strain JGDW-1

[0032] The degrading fungal strain JGDW-1 was screened from a straw accumulation place in Weifang City, Shandong Province. The suspension of the soil samples diluted step by step was applied to the CMC-Congo red plate by the gradient dilution coating method, and cultured at 10°C to screen the cellulose-degrading bacteria. Purify and isolate strains that produce larger transparent circles and grow faster on CMC-Congo red plates, inoculate them on rice straw powder medium with rice straw as the only carbon source, and culture at 10°C to compare the growth rates of each strain and growth conditions, and finally obtained the fungal strain JGDW-1 that can efficiently degrade rice straw under low temperature conditions.

[0033]CMC-Congo red medium: sodium carboxymethylcellulose (CMC-Na) 10.0g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, Congo red 0.2g, agar 20g, deionized ...

Embodiment 2

[0039] Example 2 Straw Degrading Bacteria JGDW-1 Inoculum Agent

[0040] Inoculate the strain JGDW-1 into an Erlenmeyer flask containing 50 mL of rice straw powder medium (same as in Example 1), culture it statically at 10°C for 7 days, add 30 mL of sterile water to the Erlenmeyer flask, and shake it on a shaker for 30 minutes , Filter the culture with gauze, which is the spore suspension. The spore suspension was inoculated in a liquid medium with an inoculation amount of 1%, cultured at 170 r / m for 7 days at 10°C, and centrifuged to remove bacteria and impurities to obtain a crude enzyme solution. Among them, liquid medium: rice straw powder 20g, (NH 4 ) 2 SO 4 1.4g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.3g, CaCl 2 2H 2 O 0.4 g, tryptone 1 g, FeSO 4 ·7H 2 O 0.0075g, MnSO 4 ·H 2 O 0.0025g, ZnSO 4 0.002g, CoCl 2 0.003g, 1000mL deionized water, natural pH, autoclave at 121℃ for 20min. The rice straw powder is dried rice until the water content is below 1%, crushed, ...

Embodiment 3

[0046] The degradation effect of strain JGDW-1 on rice straw was studied by liquid fermentation in a triangular flask.

[0047] Each 250mL triangular flask is divided into 3.00g of rice straw shredded to a length of 2-3cm and 150mL of liquid culture medium after being pretreated with 2% NaOH solution and dried at 50°C. Inoculate 1.5mL per bottle 10 7 The spore suspension with a concentration of 1 spore / mL was mixed and placed on a constant temperature shaker at 10°C, 20°C, 30°C, 40°C at 170r / m, and the same amount of sterile water was inoculated as a blank control. Regularly observe the morphological changes of rice straw. After 7 days, wash off the bacteria on the straw, dry to constant weight, and measure its degradation rate.

[0048] The results showed that after inoculation at 10 and 20°C for 24 hours, the culture solution began to turn gray-black, and at this time, the rice straw began to soften and disintegrate and disperse. After 48 hours, it has completely turned bl...

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Abstract

The invention discloses a low-temperature straw-degrading fungus JGDW-1, which is classified as Aureobasidium pullulans, and was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on March 22, 2018, with the preservation number CGMCC NO .15390. The bacterial strain of the invention can produce degradation circles on CMC-Congo red culture medium, and can grow on the culture medium with crop stalks as the only carbon source. The strain of the invention can produce enzymes related to the degradation of cellulose and hemicellulose, convert the macromolecular substances in the crop straw into small molecular substances that can be absorbed and utilized by plants, and realize the resource utilization of agricultural waste under low temperature conditions. with broadly application foreground. Experiments showed that after rice straw was inoculated with spore suspension, the degradation rate of rice straw was 47.3%.

Description

technical field [0001] The invention belongs to the field of agricultural resources and environment, in particular to the field of resource utilization of solid waste, and relates to a low-temperature (10-20°C) straw-degrading fungus JGDW-1 and its bacterial agent. The fungus JGDW-1 and its bacterial agent are specially used for Degrade waste crop stalks to produce organic fertilizers and realize the resource utilization of agricultural waste. Background technique [0002] my country is a large agricultural country, which generates a large amount of agricultural wastes every year, such as wheat, rice, corn and other crop straws. On the one hand, these straws contain a large amount of nutrients necessary for plant growth, such as C, but they mainly exist in the form of cellulose, which cannot be directly used by plants due to their large molecular weight. On the other hand, the application of chemical fertilizers in recent decades has seriously damaged the health of the soil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/645
CPCC05F11/08C12N1/14C12N1/145C12R2001/645
Inventor 徐阳春暴彦灼万金鑫韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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