Application of targeting optineurin in the preparation of drugs for the prevention and treatment of autoimmune diseases
An autoimmune, drug-based technology that can be used in medicine to solve problems such as death and disability
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Embodiment 1
[0025] Peripheral blood samples were collected from 30 cases of MS and RA respectively. Peripheral blood mononuclear cells (PBMC) were obtained by Ficoll gradient separation, and the mRNA level of Optn was detected by qRT-PCR. see figure 1 , the mRNA level of Optn in PBMC of MS and RA patients was higher than that of healthy controls (Healthy Donor, HD).
Embodiment 2
[0027] Using WT and Optn - / - The EAE model was established in C57BL / 6 adult female mice. The specific EAE modeling method is as follows: MOG 35-55 It was dissolved in sterile PBS and mixed with complete Freund's adjuvant containing Mycobacterium tuberculosis to prepare an emulsion. The emulsion was subcutaneously injected into the neck of the mice and the left and right hind limbs of the mice, and pertussis toxin was injected into the tail vein at the same time. On the second day after modeling, pertussis toxin was injected into the tail vein again. The scoring criteria of EAE simulated clinical multiple sclerosis symptoms were as follows: 0 points, no symptoms; 1 point, tail weakness; 2 points, tail weakness and partial limb weakness; 3 points, paralysis of one hind limb; 4 points, paralysis of both hind limbs; 5 points, moribund or dead. From the day of modeling (denoted as Day 0), EAE symptoms were scored every day. And at the end of the disease, the mouse spinal cord ...
Embodiment 3
[0030] Extract WT and Optn - / - Bone marrow-derived dendritic cells (BMDC) of mice were given LPS, and the cells were harvested 24 hours later for co-staining with CD11C and CD80, and MHC I or MHC II was used to detect the maturation of dendritic cells. see image 3 A-C, Knockdown of Optn inhibits dendritic cell maturation.
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