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Application of terminalia bellerica extract and terminalia bellerica polyphenol

An extract and drug technology, applied in the field of medicine, can solve the problems of difficult radical cure, prolonged disease course, damage, intestinal perforation and even intestinal cancer, etc., to achieve the effect of treating enteritis and relieving inflammatory reaction.

Inactive Publication Date: 2020-11-24
JINING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the pathogenesis still has many unknown areas, the whole course of the disease is protracted and difficult to cure
In the past, limited by the economic and scientific level, medical treatment in some areas of my country did not pay much attention to IBD. Many patients were only diagnosed when recurrent enteropathy caused irreversible intestinal damage, intestinal perforation or even intestinal cancer. As a result, they needed treatment for life , bringing great suffering to oneself and the family, so it is very urgent to find new and effective enteritis treatment drugs

Method used

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  • Application of terminalia bellerica extract and terminalia bellerica polyphenol
  • Application of terminalia bellerica extract and terminalia bellerica polyphenol
  • Application of terminalia bellerica extract and terminalia bellerica polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Pulverize the medicinal material of myrobalan, use 10 times the amount of water-containing organic solvent 90% ethanol to extract 3 times, each time for 10 hours, filter and recover and concentrate under reduced pressure at -0.08~-0.09MPa, 45°C.

[0046] 2. Collect the above extracts, add 2 times of water to dilute and centrifuge at 3000rpm / min for 30min, then use macroporous resin column to absorb and elute, -0.08~-0.09MPa, recover under reduced pressure at 45°C, concentrate and dry, which is Maohe sub-extract.

Embodiment 2

[0047] Embodiment 2 enteritis model construction and treatment

[0048] Fifteen male C57BL / 6 mice of 4-5 weeks were divided into 3 groups: negative control group, model group, and treatment group with chebula extract, 5 mice in each group.

[0049] The experimental animals in the model group and the treatment group of Myrobalan extract were induced by the chemical medium dextran sodium sulfate (DSS) to establish the IBD model: after the mice were weighed, they were given 4% DSS solution in exchange for normal drinking water, and the mice were observed daily. Rat feces were shaped and collected for occult blood test detection. After 5 days, if there is bloody stool, weight loss, granulocyte infiltration or strong positive occult blood test, the modeling is successful. HE staining and DAI scoring are performed to observe the inflammatory damage of the colonic mucosa and then used in the experiment.

[0050] The experimental animals depending on the treatment group of myrobalan ...

Embodiment 3

[0052] Example 3 Enteritis Model Dendritic Cell Extraction

[0053] Male 4-5 week old C57BL / 6 mice or IBD model mice were sacrificed and soaked in 75% alcohol for 10 minutes. The femur and tibia were dissected out, and the bone marrow cavity was washed repeatedly with RMPI1640 medium until the bone turned white. After centrifugation, the middle buffy coat was taken, and the cells were resuspended with the complete medium of bone marrow-derived dendritic cells (RMPI1640 medium containing 500 U / ml rmGM-CSG and rmIL-4), and the cells were mixed in 2×10 6 The density of each well was divided into 6-well plates. Change the medium every other day, add the same concentration of cytokines, and keep the suspended cells as much as possible. On day 6, all suspension cells were collected, and the expression of CD11C, a molecule on the surface of dendritic cells, was detected by flow cytometry to evaluate the purity of BMDC dendritic cells and used for experiments. Flow cytometry results...

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Abstract

The invention relates to the technical field of medicines, in particular to application of a terminalia bellerica extract and terminalia bellerica polyphenol. The invention relates to the applicationof the terminalia bellerica extract to preparation of a drug for treating inflammatory bowel disease and the application of the terminalia bellerica extract to preparation of a drug for inhibiting maturation of dendritic cells. According to the invention, the terminalia bellerica extract and the terminalia bellerica polyphenol can inhibit maturation of the dendritic cells, can effectively relieveinflammatory reaction and have the function of treating enteritis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of a myrobalan extract and polyphenols of myrobalan. Background technique [0002] Myrobalan is the dried and mature fruit of the Viriel plant Terminalia bellerica (Gaert.) Roxb. (TB). The chemical components include phenolic acids, tannins, lignans and terpenes. As a commonly used Tibetan medicine, it can resist Oxidation resists inflammation and inhibits tumors. Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a kind of chronic non-specific intestinal inflammatory disease clinically manifested as recurrent abdominal pain, diarrhea and mucus, pus and blood in the stool, and its pathogenic factors are related to immunity, genetics and environment. The incidence rate in our country has increased substantially. Because the pathogenesis still has many unknown areas, the whole course of the disease is protracted and difficult to cure. In the past, l...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P1/00A61P29/00A61P37/06A61K131/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P1/00A61P29/00A61P37/06
Inventor 严静于伟崔安芳陈飞朱宝亮成洪聚
Owner JINING MEDICAL UNIV
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