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Selenium-rich germanium-rich broccoli bud seedling soilless culture nutrient solution and preparation method thereof

A technology of broccoli sprouts and soilless cultivation, which is applied in the direction of inorganic fertilizers, liquid fertilizers, applications, etc., can solve the problems of low content of selenium and germanium elements, increase the content of selenium and germanium elements, improve the nutritional value of food, and guarantee The effect of balanced nutrition

Pending Publication Date: 2019-03-08
江苏鹤莱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the fact that the main active ingredient of broccoli sprouts is sulforaphane, although previous studies can increase the content of sulforaphane, the content of selenium and germanium in the sprouts is very small.

Method used

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  • Selenium-rich germanium-rich broccoli bud seedling soilless culture nutrient solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0051] A kind of Selenium-enriched germanium-enriched nutrient solution formula for soilless cultivation of broccoli sprouts. In each liter of nutrient solution, its components and contents are as follows:

[0052] Scheme 1: The dosage of potassium dihydrogen phosphate and dipotassium hydrogen phosphate can be adjusted according to the measured pH value.

[0053] Potassium dihydrogen phosphate 10-125mg / L, dipotassium hydrogen phosphate 10-150mg / L, zinc sulfate 100.55mg / L, L-selenomethionine 25mg / L, L-selenium-methylselenocysteine ​​25mg / L L, germanium stone powder 200mg / L, sodium salicylate 50.00mg / L, calcium propionate 1800.00mg / L, calcium nitrate tetrahydrate 500.45 mg / L, ammonium nitrate 120.20 mg / L, ammonium sulfate 120.52 mg / L, potassium sulfate 60.44 mg / L, the rest is water, adjust the pH to 6.0, as a concentrated stock solution, filter through a 0.45μ membrane, and store at 4°C for a short period of one week. Before use, take the supernatant mother solution and dilute ...

Embodiment 2

[0058] Embodiment 2, broccoli sprout cultivation:

[0059] (1) Choose CDBY-10 broccoli seeds with full grains, high purity and cleanliness without mildew as the seeds for sprout seedling cultivation;

[0060] (2) Disinfection: soak the seeds of step (1) with 5g / L sodium hypochlorite solution for 2 hours, then rinse with sterile water for 3-4 times to obtain sterilized seeds;

[0061] (3) Take several clean and non-toxic plastic trays, and place double layers of gauze or rough-holed paper on them;

[0062] (4) Put the sterilized seeds on the gauze, add 1 x nutrient solution to soak the gauze or paper, and add selenium-free and germanium-free nutrient solution to soak the gauze in the control. The formula of selenium-free and germanium-free nutrient solution is as follows:

[0063] Potassium dihydrogen phosphate 20mg / L, dipotassium hydrogen phosphate 141.61 mg / L, zinc sulfate 100.55mg / L, sodium salicylate 50.00mg / L, calcium propionate 1800.00mg / L, calcium nitrate tetrahydrate ...

Embodiment example 3

[0065] Implementation Case 3, Determination of Selenium in Broccoli Sprouts

[0066] According to the method of "National Food Safety Standards Determination of Selenium in Food" (GB5009.93-2017) of the People's Republic of China. The measuring instrument used is atomic fluorescence spectrometer (with selenium hollow cathode lamp).

[0067] 1) Sample preparation: Fresh samples of broccoli sprouts were washed with water, dried, and the edible part was taken to make a homogenate.

[0068] 2) Sample digestion: wet digestion.

[0069] Weigh 0.5g~3g of solid sample (accurate to 0.001g) or accurately pipette 1.00mL~5.00mL of liquid sample, put it in a conical flask, add 10mL of nitric acid-perchloric acid mixed acid (9+1) and A few glass beads, covered with a watch glass, cold digested overnight. The next day, heat it on a hot plate, and add nitric acid in time. When the solution becomes clear and colorless accompanied by white smoke, continue to heat until the remaining volume ...

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Abstract

The invention belongs to the technical field of production of raw materials of health food and specifically relates to a preparation method for a broccoli bud seedling soilless culture nutrient solution. A broccoli bud seedling rich in sulforaphane is cultured with the soilless culture nutrient solution prepared from the following components: 0-125 mg / L monopotassium phosphate, 0-150 mg / L dipotassium phosphate, 10-200 mg / L zinc sulfate, 1-50 mg / L L-selenomethionine, 1-50 mg / L L-se-methylselenocysteine, 100-1000 mg / L germanite powder, 5-100 mg / L sodium salicylate, 250-2000 mg / L calcium propionate, 900-1100 mg / L calcium nitrate tetrahydrate, 100-250 mg / L ammonium sulfate, 50-260 mg / L potassium sulfate and the balance of water. The pH is adjusted to 5.5-6.5. Before use, supernatant mother liquor is taken and diluted for 100 times with sterile water.

Description

Technical field: [0001] The invention belongs to the technical field of production of health food raw materials, and in particular relates to a nutrient solution for soilless cultivation of broccoli sprout seedlings rich in selenium and germanium. Background technique: [0002] Selenium is a chemical element with the chemical symbol Se, which is located in Group VI A of the fourth period of the chemical element periodic table and is a non-metallic element. [0003] Selenium is divided into plant active selenium and inorganic selenium. Inorganic selenium generally refers to sodium selenite and sodium selenate. Inorganic selenium is highly toxic and not easily absorbed, so it is not suitable for human and animal use. [0004] Plant-active selenium is formed through biotransformation and amino acid combination, and generally exists in the form of selenomethionine. Plant-active selenium is a selenium source that is allowed for humans and animals. L-selenium-methylselenocystein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
CPCC05B7/00C05G3/00C05G5/23C05C3/00C05C5/04C05C11/00C05D1/02C05D9/02C05F11/00
Inventor 李万浪李经
Owner 江苏鹤莱科技有限公司
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