Benzene series degradation bacteria FB1, as well as screening method thereof, and application in degradation of the benzene series

A technology of benzene series degrading bacteria and screening methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of low treatment efficiency and negative environmental impact

Active Publication Date: 2019-03-01
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned prior art, the purpose of the present invention is to overcome the problems of low processing efficiency and other negative impacts on the environment in the prior art for the treatment of benzene series, thereby providing a simple treatment process that can treat benzene series The soil and water polluted by pollutants are biodegraded, converted into harmless substances and discharged into the environment. There is no additional discharge of harmful substances, and the negative impact on the environment is the lowest. It is safe, economical, efficient, no secondary pollution, and does not require large-scale Benzene series degrading bacteria FB1 with simple equipment and operation and high degradation rate, its screening method and its application in degrading benzene series

Method used

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  • Benzene series degradation bacteria FB1, as well as screening method thereof, and application in degradation of the benzene series
  • Benzene series degradation bacteria FB1, as well as screening method thereof, and application in degradation of the benzene series
  • Benzene series degradation bacteria FB1, as well as screening method thereof, and application in degradation of the benzene series

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preparation example Construction

[0024] 1) Preparation of the liquid medium: mixing the basal medium, the mixed solution of trace elements and the mixed solution of benzene series to prepare the liquid medium;

[0025] 2) Enrichment and acclimatization: Take contaminated soil samples around the coking plant and add them to the liquid medium prepared in part of step 1) for mixing to obtain the first mixed solution; then transfer the first mixed solution to another part of step 1) In the liquid culture medium prepared in the above method, the second mixed solution was obtained; the second mixed solution was transferred and subjected to domestication culture to obtain the domesticated bacterial solution;

[0026] 3) Isolation and purification: the acclimatized bacteria solution was diluted and cultured to obtain benzene series degrading bacteria FB1.

[0027] Further, the amount of the polluted soil samples around the coking plant and the liquid culture medium prepared in some steps 1) can be adjusted according ...

Embodiment

[0057] Enrichment and acclimatization: Collect soil in the polluted area next to the coking plant, take 10g of soil and add it to the liquid medium, shake it well to make the first mixed solution, and then mix the above first mixed solution with the liquid medium at a ratio of 1:10 Inoculate into liquid medium by volume ratio; under the conditions of 25°C and 150r / min, transfer once every 8 days for acclimatization culture, a total of 7 transfers (250mL serum bottles are used during the cultivation process, sealed with special bottle caps) , to prevent volatilization of benzene series);

[0058] Separation and purification: Take 1 mL of the above-mentioned domesticated bacterial liquid and add it to 9 mL of distilled water, and record the concentration of the bacterial liquid as 10 -1 ; then from a concentration of 10 -1 Take 1mL of the bacterial solution and add it to 9mL distilled water, and the concentration of the bacterial solution is recorded as 10 -2 ; dilute sequenti...

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Abstract

The invention discloses benzene series degradation bacteria FB1, as well as a screening method thereof, and an application in degradation of the benzene series. The benzene series degradation bacteriaFB1 belong to Pseudomonas viridilivida, assigned the accession number CCTCC No: M2018347. The screening method includes: enriching, acclimatizing, separating and purifying the bacteria from contaminated soil sample around a coking plant. In the invention, the soil is extracted from benzene series contaminated farmlands around the coking plant and the benzene series degradation bacteria FB1 are screened out from the soil, so that by means of the FB1, the benzene series contaminated soil can be biodegraded, thus purifying and remediating the soil. The method is low in operation cost, is insignificant in negative influence on environment, and has excellent development and application prospect.

Description

technical field [0001] The invention relates to the technical field of biodegradation treatment of soil and water, in particular to the benzene series degrading bacteria FB1 and its screening method and its application in degrading benzene series. Background technique [0002] The three isomers of benzene, toluene, ethylbenzene and xylene are collectively called benzene series, which are important volatile organic pollutants in the environment. Benzene series are monocyclic aromatic hydrocarbons, which mainly exist in crude oil and petroleum products, and are also widely used as industrial raw materials. Benzene series entering the environment will cause the following effects: (1) Benzene series are volatile and easily enter the atmosphere, participate in photochemical reactions, promote ozone generation, and further aggravate the formation of photochemical smog; (2) Benzene series are water-soluble When it enters the water body, it will form a thin film on the surface of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B09C1/10C02F3/34C02F101/32C12R1/38
CPCB09C1/10C02F3/34C02F2101/32C12N1/02C12N1/20C12N1/205C12R2001/38
Inventor 张浩吴婷章慧姚沐岑刘辉桂光照田文凤高亢
Owner ANHUI NORMAL UNIV
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