Extraction method of coriolus versicolor beta-glucan peptide and application of coriolus versicolor beta-glucan peptide to obesity or related diseases

Abstract

The invention discloses an extraction method of coriolus versicolor beta-glucan peptide and application of the coriolus versicolor beta-glucan peptide to obesity or related diseases. The extraction method comprises the following steps: washing coriolus versicolor sporophore or spore powder and drying; extracting for 3 times by utilizing water at 100 DEG C, wherein the water which is added for thefirst time is 15 times of coriolus versicolor and the water which is added for the second time is 10 times of the coriolus versicolor; combining extracting solutions of two times; concentrating and adding ethanol to carry out alcohol precipitation; when the alcohol precipitation is carried out for 24 h, filtering and collecting sediment; centrifuging, freezing and drying sediment to obtain a coriolus versicolor polysaccharide crude product; eluting the coriolus versicolor polysaccharide crude product through water, a sodium chloride water solution and the sodium chloride water solution in sequence on a DEAE-Sepharose-fast-flow column; tracking an elution curve by utilizing a sulfuric acid-phenol method, and collecting effective parts obtained by eluting through the sodium chloride water solution and the sodium chloride water solution respectively; concentrating, dialyzing, concentrating and freeze-drying effective partscomponents which are the effective parts components collected and eluted according to the elution curve, so as to obtain a series of beta-glucan peptide. The polysaccharide peptide has the effect of alleviating or controlling the obesity or the related diseases.

Description

Technical field [0001] The present invention relates to the technical field of plant extraction, in particular to a method for extracting Yunzhi β-glucan peptide and its application in obesity or related diseases. Background technique [0002] Yunzhi is the fruiting body or mycelium of Yunzhi, a large and precious medicinal fungus, which is used for anticancer treatment. Yunzhi is cold in nature, slightly sweet in taste, and has heat-clearing and anti-inflammatory effects. Yunzhi polysaccharide is the main active ingredient in Yunzhi extract: the molecular weight is 1.3×10 6 Above, it is a glucan containing β-glycosidic bonds, and it is determined to be β(1→4), β(1→3) and β(1→6) glycosidic bonds. The main active ingredient of Yunzhi extract is Yunzhi polysaccharide. The pharmacological effect of Yunzhi polysaccharide: It has the effect of removing dampness and reducing phlegm, and can treat lung disease and chronic bronchitis, persistent or chronic hepatitis. The main active i...

AI Technical Summary

Problems solved by technology

However, polysaccharide peptides such as versicolor versicolor are highly polar, and are difficult to be absorbed into the blood when taken orally; therefore, the molecular mechanism of how this polysaccharide exerts the above functions is still unclear

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