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A kind of Eucheuma hypolipidemic tetrapeptide and its application

A technology for lowering blood lipids and kylin cabbage, which is applied in the application, tetrapeptide components, peptides and other directions to achieve the effect of good lipid-lowering activity, good blood-lipid lowering activity, and reducing fat accumulation.

Active Publication Date: 2020-12-15
清枫链食苏打饮品(吉林)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, using Eucheuma as raw material, the process research on the preparation of Eucheuma hypolipidemic oligopeptides by enzymatic hydrolysis technology is in a blank stage

Method used

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  • A kind of Eucheuma hypolipidemic tetrapeptide and its application
  • A kind of Eucheuma hypolipidemic tetrapeptide and its application
  • A kind of Eucheuma hypolipidemic tetrapeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A tetrapeptide for lowering blood lipids from unicornis, the amino acid sequence of which is Phe-Tyr-Lys-Ala (FYKA), the molecular weight measured by ESI-MS is 527.50 Da, and has good lipid-lowering activity. The Phe-Tyr-Lys-Ala (FYKA) tetrapeptide Phe-Tyr-Lys-Ala (FYKA) can significantly reduce the fat accumulation of hepatocytes induced by oleic acid, and significantly reduce the amount of hepatocytes induced by oleic acid. Internal triglyceride (TG) and total cholesterol (TC) levels indicated that Phe-Tyr-Lys-Ala (FYKA) had better lipid-lowering activity. Therefore, Phe-Tyr-Lys-Ala (FYKA) has good hypolipidemic activity and can be used for the development of hypolipidemic-related medicines, health care products, and special medical foods.

[0032] A preparation method of tetrapeptide for lowering blood lipids of cylindrica 0.3%(W / V)CaCl 2 Solution, CaCl 2 Solution contains CaCl 2 2.8% ethacridine lactate and 15.8% potassium nitrate by weight. The appropriate con...

Embodiment 2

[0044] A tetrapeptide for lowering blood lipids from unicornis, the amino acid sequence of which is Phe-Tyr-Lys-Ala (FYKA), the molecular weight measured by ESI-MS is 527.50 Da, and has good lipid-lowering activity.

[0045] A preparation method of tetrapeptide for lowering blood lipids of cylindrica 0.1%(W / V)CaCl 2 Solution, CaCl 2 Solution contains CaCl 2 2.5% ethacridine lactate and 15.0% potassium nitrate by weight.

[0046] The concrete steps of the preparation method of above-mentioned kylin cabbage hypolipidemic tetrapeptide are:

[0047] 1) Preparation of Cylindrocystis algal protein: take Cylindrocystis powder, add extraction solvent according to the ratio of material to liquid 1g:100mL, soak for 8 hours, extract 30min with frequency 50kHz ultrasonic at room temperature, centrifuge the extract, take the supernatant and add ammonium sulfate to When the saturation reaches 10%, fully stir for 20 minutes and then freeze and centrifuge. Take the supernatant and continu...

Embodiment 3

[0054] A tetrapeptide for lowering blood lipids from unicornis, the amino acid sequence of which is Phe-Tyr-Lys-Ala (FYKA), the molecular weight measured by ESI-MS is 527.50 Da, and has good lipid-lowering activity.

[0055]A preparation method of tetrapeptide for lowering blood lipids of cylindrica 0.5%(W / V)CaCl 2 Solution, CaCl 2 Solution contains CaCl 2 3.0% ethacridine lactate and 16.0% potassium nitrate by weight.

[0056] The concrete steps of the preparation method of above-mentioned kylin cabbage hypolipidemic tetrapeptide are:

[0057] 1) Preparation of Cylindrocystis algal protein: take Cylindrocystis powder, add extraction solvent according to the ratio of material to liquid 1g:100mL, soak for 12h, extract 60min with frequency 60kHz ultrasonic at room temperature, centrifuge the extract, take the supernatant and add ammonium sulfate to When the saturation reaches 10%, fully stir for 25min and then freeze and centrifuge. Take the supernatant and continue to add a...

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Abstract

The invention provides a eucheuma blood fat reducing tetrapeptide and an application thereof, and belongs to the technical field of blood pressure reducing oligopeptide. The amino acid sequence of theeucheuma blood fat reducing tetrapeptide is Phe-Tyr-Lys-Ala (FYKA), and the eucheuma blood fat reducing tetrapeptide has good fat reducing activity. The eucheuma blood fat reducing tetrapeptide can be applied to preparation of blood fat reducing drugs, health care products and food additives. The eucheuma blood fat reducing tetrapeptide can remarkably reduce fat accumulation amount of oleic acidinduced hepatic cells, remarkably reduces the level of triglyceride (TG) and total cholesterol (TC) in the hepatic cells, has good blood fat reducing activity and can be used for developing the bloodfat reducing related drugs, the health care products and special medical food.

Description

technical field [0001] The invention belongs to the technical field of blood lipid-lowering oligopeptides, and in particular relates to a blood fat-lowering tetrapeptide of Eucheuma japonicus and its application. Background technique [0002] Abnormal fat metabolism or operation makes one or more lipids in plasma higher than normal value, which is called hyperlipidemia. At present, in China, hyperlipidemia is generally diagnosed as adult fasting serum total cholesterol exceeding 5.72 mmol / L and triglyceride exceeding 1.70 mmol / L. Those whose total cholesterol is between 5.2 and 5.7 mmol / L are called marginally elevated. Hyperlipidemia can be divided into two types: primary and secondary. Primary is related to congenital and heredity, most of which are caused by abnormality of receptors, enzymes or apolipoproteins involved in lipoprotein transport and metabolism due to single or multiple gene defects, or due to environmental factors (diet, nutrition, drugs) ). Secondary m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/107C07K1/36C07K1/34C07K1/20C07K1/16C12P21/06A61K38/07A61P3/06A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P3/06C07K5/1016C12P21/06A23V2200/326A23V2250/55
Inventor 王斌李雪荣孙坤来
Owner 清枫链食苏打饮品(吉林)有限公司
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