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Avian mycoplasma culture medium, preparation method of avian mycoplasma solution and applications of avian mycoplasma culture medium and avian mycoplasma solution

A technology of avian mycoplasma and liquid culture medium, which is applied in the field of avian mycoplasma culture medium, can solve the problems of unstable vaccine product quality, low antigen content and purity, and low concentration of cultured bacteria, so as to reduce the incidence of vaccine immune side effects, Save manpower and achieve high antigen purity

Active Publication Date: 2019-02-26
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production of mycoplasma avium vaccine mainly uses improved Frey's medium, CM2 medium, etc. to cultivate, and these mediums have a higher serum concentration (generally more than 10%), and the concentration of the cultured bacteria solution is not high (generally 10%). 8-10 CCU / ml), the strains need to be reproduced for each production, the production operation is cumbersome, the cost is high, the quality of the vaccine product is unstable (antigen content and purity are not high), and side reactions are prone to occur

Method used

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  • Avian mycoplasma culture medium, preparation method of avian mycoplasma solution and applications of avian mycoplasma culture medium and avian mycoplasma solution
  • Avian mycoplasma culture medium, preparation method of avian mycoplasma solution and applications of avian mycoplasma culture medium and avian mycoplasma solution
  • Avian mycoplasma culture medium, preparation method of avian mycoplasma solution and applications of avian mycoplasma culture medium and avian mycoplasma solution

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Embodiment 1

[0024] A kind of mycoplasma avium culture medium of the present invention, described mycoplasma avium culture medium is made up of mycoplasma avium basal culture medium and auxiliary component, and described basal culture medium is conventional liquid culture medium of mycoplasma avium, and described auxiliary component is composed of gelatin, Composed of Tween 80 and calcium chloride.

[0025] The basal medium is modified Frey's medium.

[0026] Described auxiliary component consumption is the following component composition of finished medium mass volume ratio (m:V):

[0027] Gelatin 0.2-0.4%,

[0028] Tween 80 0.5-1%,

[0029] Calcium chloride 0.2-0.4%.

[0030] A kind of preparation method of mycoplasma avium bacterium liquid of the present invention, the bacterial classification used for production is mycoplasma gallisepticum or mycoplasma gallisepticum, and the bacterium liquid comprises the following steps:

[0031] (1) Inoculate the qualified seeds for Mycoplasma a...

Embodiment 2

[0037] The difference between embodiment 2 and embodiment 1 is: a kind of mycoplasma avium culture medium of the present invention, described mycoplasma avium culture medium is made up of mycoplasma avium basal culture medium and auxiliary component, and described basal culture medium is conventional mycoplasma avium liquid Medium, the auxiliary components are composed of gelatin, Tween 80 and calcium chloride.

[0038] The basal medium is CM medium.

[0039] Described auxiliary component consumption is the following component composition of finished medium mass volume ratio (m:V):

[0040] Gelatin 0.2-0.4%,

[0041] Tween 80 0.5-1%,

[0042] Calcium chloride 0.2-0.4%.

[0043] A kind of preparation method of mycoplasma avium bacterium liquid of the present invention, the bacterial classification used for production is mycoplasma gallisepticum or mycoplasma gallisepticum, and the bacterium liquid comprises the following steps:

[0044] (1) Inoculate the qualified seeds for...

Embodiment 3

[0048] The difference between embodiment 3 and embodiment 1 is: a kind of mycoplasma avium culture medium of the present invention, described mycoplasma avium culture medium is made up of basic culture medium of mycoplasma avium and auxiliary component, and described basic culture medium is conventional mycoplasma avium liquid Medium, the auxiliary components are composed of gelatin, Tween 80 and calcium chloride.

[0049] The basal medium is conventional CM2 liquid medium.

[0050] Described auxiliary component consumption is the following component composition of finished medium mass volume ratio (m:V):

[0051] Gelatin 0.2-0.4%,

[0052] Tween 80 0.5-1%,

[0053] Calcium chloride 0.2-0.4%.

[0054] A kind of preparation method of mycoplasma avium bacterium liquid of the present invention, the bacterial classification used for production is mycoplasma gallisepticum or mycoplasma gallisepticum, and the bacterium liquid comprises the following steps:

[0055] (1) Inoculate...

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Abstract

The invention relates to an avian mycoplasma culture medium, a preparation method of an avian mycoplasma solution and applications of the avian mycoplasma culture medium and the avian mycoplasma solution, and belongs to the technical field of biology. The avian mycoplasma culture medium comprises a basal culture medium and auxiliary components, wherein the basal culture medium is a conventional avian mycoplasma liquid culture medium, and the auxiliary components comprise gelatin, Tween 80 and calcium chloride; a strain for production is mycoplasma galliscepticum or mycoplasma synoviae, the avian mycoplasma solution is produced by solution inoculation, culture and harvesting, and an avian mycoplasma vaccine is prepared. According to the preparation method, antigen content of the avian mycoplasma solution is increased, production technology is simplified, production cost is reduced, probability of occurrence of vaccine immunity side effects is reduced, and vaccine immunity effect is improved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of a mycoplasma avium culture medium, a mycoplasma avium bacterial liquid and an application thereof. Background technique [0002] Avian Mycoplasmosis (Avian Mycoplasmosis, AM) is an infectious disease of poultry caused by Mycoplasma avium. The common clinical pathogens are Mycoplasma Gallisepticum (MG) and Mycoplasma Synoviae (MS). Mycoplasma gallisepticum infection can cause Chronic Respiratory Disease (CRD), air sacculitis and sinusitis in poultry; Mycoplasma gallisepticum infection can cause symptoms such as joint swelling, synovial bursa and tendon inflammation, and parenchymal organ enlargement; Effective vaccine immunization is the main means of preventing and controlling the disease. [0003] At present, the production of mycoplasma avium vaccine mainly uses improved Frey's medium, CM2 medium, etc. to cultivate, and these mediums have a higher s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCA61K39/0241A61P31/04C12N1/20A61K2039/552A61K2039/522
Inventor 何平有朱秀同赵玉龙刘涛郁宏伟高晓磊焦玉兰刘浩邱贞娜张新新李卫东赵丽霞
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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