Method for producing liquid edible fungus strains by using wastes from cordyceps militaris culture medium

A technology of medium and liquid strains of Cordyceps militaris, applied in the direction of botany equipment and methods, cultivation, application, etc., which can solve the problem of lack of reuse channels for medium waste, pollution of natural ecological environment and reproduction environment, and restriction of Cordyceps industry. Sustainable development and other issues to achieve the effect of facilitating large-scale factory production, high safety indicators, and low cost

Active Publication Date: 2019-02-26
LIAONING SANYOU AGRI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The culture medium waste lacks an effective reuse method, pollutes the natural ecological environm...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1), crush the medium waste produced in the cultivation of Cordyceps militaris to 40-60 mesh, add water according to the ratio of material to water (mass volume ratio of medium waste to water) 1:8g / mL and stir well, boil water Gelatinization for 20min to obtain culture medium waste gelatinized material;

[0025] (2) Cool the medium waste gelatinized material to 60°C, adjust the pH value to 4.5 with dilute hydrochloric acid with a mass fraction of 18%, and add dual enzymes according to the amount of α-amylase 4U / g and glucoamylase 6U / g Mix and stir, wherein the mass ratio of α-amylase and glucoamylase is 1:1.8, perform double enzyme digestion at 60°C, react for 40min, raise the temperature to 90°C and maintain for 10min, to terminate the enzymatic reaction;

[0026] (3), be cooled to 55 ℃ of temperature, add water to the original volume of culture medium waste gelatinized material, the dilute hydrochloric acid of mass fraction 18% and NaOH solution adjust pH value to 7.0...

Embodiment 2

[0035] (1) Crush the culture medium waste produced in the planting of Cordyceps militaris to 40-60 mesh, add water according to the ratio of material to water 1:6g / mL and stir well, and gelatinize in boiling water for 20 minutes to obtain the culture medium waste gelatinized material ;

[0036] (2) Cool the medium waste gelatinized material to 55°C, adjust the pH value to 4.0 with dilute hydrochloric acid with a mass fraction of 18%, and add dual enzymes according to the amount of α-amylase 4U / g and glucoamylase 6U / g Mix and stir, wherein the mass ratio of α-amylase and glucoamylase is 1:1.5, perform double enzyme digestion at 55°C, react for 40 minutes, raise the temperature to 90°C for 5 minutes, and terminate the enzymatic reaction;

[0037] (3), be cooled to temperature 55 ℃, add water to the original volume of culture medium waste gelatinized material, the dilute hydrochloric acid of mass fraction 18% adjusts pH value 5.0, add and account for the papain of culture medium ...

Embodiment 3

[0046](1) Crush the culture medium waste produced in the cultivation of Cordyceps militaris to 40-60 mesh, add 7mL of water per gram of culture medium waste, that is, the ratio of material to water is 1:7g / mL, add water and stir well, and gelatinize in boiling water 20min, obtain medium waste gelatinized material;

[0047] (2) Cool the culture medium waste gelatinized material to 58°C, adjust the pH value to 4.2, add the double enzymes according to the amount of α-amylase 4U / g, glucoamylase 6U / g, mix and stir, wherein, α - The mass ratio of amylase and glucoamylase is 1:1.7, and double enzyme digestion is carried out at 58°C. After 40 minutes of reaction, the temperature is raised to 90°C and maintained for 8 minutes to terminate the enzymatic reaction;

[0048] (3), cooling to a temperature of 55° C., replenishing water to the original volume of the medium waste gelatinized material, adjusting the pH value to 6, adding papain accounting for 0.15% of the medium waste, and the ...

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Abstract

The invention provides a method for producing liquid edible fungus strains by using wastes from a cordyceps militaris culture medium. The method comprises the steps that the culture medium wastes generated during cordyceps militaris planting are smashed and stirred to be uniform by adding water, after gelatinization with boiling water, cooling is conducted, and the pH value is adjusted; the smashed wastes are mixed with alpha-diastasum diastace and glucoamylase and then stirred, and after a double-enzyme digestion reaction, an enzymatic reaction is stopped; cooling is conducted, water is replenished, the pH value is adjusted, papain is added, and an enzymatic reaction is carried out; after the enzymatic reaction, solid-liquid separation is conducted to remove residues, and a culture mediumwaste hydrolysis fluid is obtained; the hydrolysis fluid is diluted with water, after sterilization and fermentation, the diluted culture medium waste hydrolysis fluid is directly used for productionof edible fungi. The method has the advantages that being decomposed by cordyceps taishanensis exoenzyme, the wastes are suitable for culture raw materials of other macro fungi, and the wastes are converted into culture mediums in the fermentation industry and successfully used for production of the liquid strains of aurcularia auricula or lentinula edodes or hericium erinaceus or pleurotus citrinopileatus. The method has the advantages that the raw materials are low in cost and easy to obtain, the preparation process is simple, the wastes are changed into valuable things, and the method conforms to environmental protection concepts.

Description

technical field [0001] The invention belongs to the field of recycling and reuse of agricultural production waste resources, and relates to a method for producing edible fungus liquid strains by using waste culture medium of Cordyceps militaris, in particular to a method for producing fungus, shiitake mushrooms and Hericium erinaceus by using waste culture medium of Cordyceps militaris The method of edible fungus liquid strains such as fungus or yellow elm mushroom can realize the reutilization of the culture medium waste of Cordyceps militaris through biotransformation. Background technique [0002] Cordyceps militaris is a traditional medicinal and edible fungus, rich in nutrients. It is a fast-growing industry with a large scale. Shenyang City, Liaoning Province is the place where Cordyceps militaris was discovered and the birthplace of artificial cultivation of Cordyceps militaris. After more than 30 years of planting and development, Shenyang has an annual production c...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 邹存兵朱巍巍赵婧羽邓正正邹冬冬李颖贾维新
Owner LIAONING SANYOU AGRI BIOTECH
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