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Ratio fluorescent nanoprobe for visual detection of hypochlorite as well as preparation and application of ratio fluorescent nanoprobe

A technology for detecting hypochlorous acid and ratiometric fluorescence, applied in the field of ratiometric fluorescent nanoprobes, can solve problems such as detection application limitations, and achieve the effects of simple synthesis route, low cost investment, and convenient post-processing

Active Publication Date: 2019-02-22
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the water environment in daily life is a neutral environment with a pH of about 7, which limits its detection application in the environment and daily life.

Method used

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  • Ratio fluorescent nanoprobe for visual detection of hypochlorite as well as preparation and application of ratio fluorescent nanoprobe
  • Ratio fluorescent nanoprobe for visual detection of hypochlorite as well as preparation and application of ratio fluorescent nanoprobe
  • Ratio fluorescent nanoprobe for visual detection of hypochlorite as well as preparation and application of ratio fluorescent nanoprobe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: The preparation method of the ratiometric fluorescent nanoprobe for visually detecting hypochlorite, the specific steps are as follows:

[0041] (1) Dissolve 4-(1,2,2-triphenylvinyl)acetophenone (0.267mmol) and 5-nitrosalicylaldehyde (0.401mmol) in 20mL of acetonitrile, add a few drops of Pyridine, placed in N 2 Under the conditions of protecting and avoiding light, reflux reaction for 12 hours, after the reaction was completed, 85-95% of the solvent was removed by rotary evaporation, the product was purified by column separation, and dried in vacuum to obtain product 1;

[0042] (2) Take the product 1 synthesized in step (1) and prepare it as a 0.02mg / mL tetrahydrofuran (THF) solution A, and take the polyethylene oxide-co-polystyrene prepared according to the patent CN201810983228.5 and prepare it as 2mg / mL Tetrahydrofuran (THF) solution B, then mix 1mL of A and B respectively, add to 10mL of water under ultrasonic conditions, continue ultrasonication fo...

Embodiment 2

[0043] Embodiment 2: The preparation method of the ratiometric fluorescent nanoprobe for visually detecting hypochlorite, the specific steps are as follows:

[0044] (1) Dissolve 4-(1,2,2-triphenylvinyl)acetophenone (0.267mmol) and 5-nitrosalicylaldehyde (0.267mmol) in 10mL of acetonitrile, add a few drops of Pyridine, placed in N 2 Under the conditions of protecting and avoiding light, reflux reaction for 8 hours, after the reaction was completed, 85-95% of the solvent was removed by rotary evaporation, the product was purified by column separation, and dried in vacuum to obtain product 1;

[0045] (2) Take the product 1 synthesized in step (1) and prepare it as a 0.02 mg / mL tetrahydrofuran (THF) solution A, and take the polyethylene oxide-co-polystyrene prepared according to the patent CN201810983228.5 and prepare it as 1.5 mg / mL mL of tetrahydrofuran (THF) solution B, then take 1mL of A and B to mix, add to 10mL of water under ultrasonic conditions, continue ultrasonicatio...

Embodiment 3

[0046] Embodiment 3: The preparation method of the ratiometric fluorescent nanoprobe for visual detection of hypochlorite, the specific steps are as follows:

[0047] (1) Dissolve 4-(1,2,2-triphenylvinyl)acetophenone (0.267mmol) and 5-nitrosalicylaldehyde (0.534mmol) in 30mL of acetonitrile, add a few drops of Pyridine, placed in N 2 Under the conditions of protecting and avoiding light, reflux reaction for 16 hours, after the reaction was completed, 85-95% of the solvent was removed by rotary evaporation, the product was purified by column separation, and dried in vacuum to obtain product 1;

[0048] (2) Take the product 1 synthesized in step (1) and prepare it as 0.02mg / mL tetrahydrofuran (THF) solution A, and take the polyethylene oxide-co-polystyrene prepared according to the patent CN201810983228.5 and prepare it as 2.5mg / mL mL of tetrahydrofuran (THF) solution B, then take 1mL of A and B to mix, add to 10mL of water under ultrasonic conditions, continue ultrasonication ...

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Abstract

The invention discloses a ratio fluorescent nanoprobe for visual detection of hypochlorite as well as preparation and application of the ratio fluorescent nanoprobe. The fluorescent nanoprobe is a novel ratio fluorescent nanoprobe prepared by taking polyethylene oxide-co-polystyrene, 4-(1,2,2-triphenylvinyl)acetophenone and 5-nitrosalicylaldehyde as raw materials according to the prior art. The fluorescent nanoprobe can be used for realizing high-selectivity and high-sensitivity quick ratio detection of the hypochlorite in a pure water solution. Compared with current fluorescent detection technology, the fluorescent nanoprobe obtained by the invention has the advantages that the high-selectivity ratio quick response and visual detection on the hypochlorite are realized, aggregation-inducedfluorescence quenching phenomena can be effectively avoided, biocompatibility and water dispersibility are excellent, invested costs are relatively low and a synthetic route is simple, and the fluorescent nanoprobe is suitable for amplified synthesis and practical production application and has broad application prospects in the technical fields such as analytic chemistry, bioscience and environmental science.

Description

technical field [0001] The invention belongs to the technical field of preparation, analysis and detection of chemical materials, and in particular relates to a ratio fluorescent nanoprobe for visually detecting hypochlorite and its preparation and application. Background technique [0002] Hypochlorite is a very important reactive oxygen species that widely exists in biological cells and plays an important role in the process of oxidative stress in biological systems. However, because of its high activity, low content, and uneven distribution, It is particularly important to explore their functions in living organisms. In addition, hypochlorite is also widely used in the disinfection treatment of domestic water, such as tap water disinfection, swimming pool water disinfection, etc. The United Nations Health Organization pointed out that for the treatment of these domestic waters, the effective free chlorine concentration should not be less than 0.2mg / L (5.6μM), because of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64C07C201/12C07C205/45C07C45/74C07C49/835
CPCG01N21/64G01N21/6428G01N21/643G01N21/6456C09K11/06C07C45/74C07C49/835C07C201/12C07C205/45C09K2211/1007G01N2021/6421G01N2021/6439
Inventor 陈建王宏林众邹朝张培盛
Owner HUNAN UNIV OF SCI & TECH
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