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Extraction method of polysaccharide from sargassum fusiforme

A hijiki polysaccharide and extraction method technology, applied in the field of hijiki polysaccharide extraction, can solve the problems of low polysaccharide yield, long production time, waste, etc., and achieve high purity, high extraction rate and high purity of polysaccharide products Effect

Pending Publication Date: 2019-02-22
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the various nutrients mainly composed of hijiki polysaccharides are water-soluble substances, so they can be extracted by hot water extraction; this extraction method is simple, feasible, easy to operate, and does not require high equipment, but its production The time is long, the polysaccharide yield is not high, and the waste is serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The present embodiment provides a method for extracting hijiki polysaccharides, comprising the following steps:

[0030] Beating: Wash hijiki, add 2 times the weight of purified water, and beat in a beater or homogenizer for 10 minutes.

[0031] Preliminary enzymatic hydrolysis: adjust the pH of the slurry to 4.2; add a compound enzyme solution to the slurry, the amount of enzyme added is 0.5mKat / g, and the enzymatic hydrolysis time is 3h. After the enzymatic hydrolysis is completed, the temperature is raised to kill the enzyme.

[0032] Fermentative degradation of specific microorganisms: raise the temperature of the raw material after enzymolysis to 37°C, adjust the pH of the material to 7.9; then add 0.05 parts of Aspergillus oryzae, Bifidobacterium, and Bacillus subtilis, mix well, and ferment for 5-10 hours.

[0033] Secondary enzymatic hydrolysis: adjust the pH of the slurry to 5.3; add a compound enzyme solution to the slurry, the enzyme addition amount is 1.5mKa...

Embodiment 2

[0041] The extraction method of hijiki polysaccharide provided by the present embodiment comprises the following steps:

[0042] Beating: Wash hijiki, add 3 times the weight of pure water, and beat in a beater or homogenizer for 20 minutes.

[0043] Preliminary enzymatic hydrolysis: adjust the pH of the slurry to 5.2; add a compound enzyme solution to the slurry, the amount of enzyme added is 2.3mKat / g, and the enzymatic hydrolysis time is 1h. After the enzymatic hydrolysis is completed, the temperature is raised to kill the enzyme.

[0044] Fermentative degradation of specific microorganisms: raise the temperature of the raw material after enzymolysis to 40°C, adjust the pH of the material to 8.3; then add 1.5 parts of Aspergillus oryzae, Bifidobacterium, and Bacillus subtilis, mix well, and ferment for 10 hours.

[0045] Secondary enzymatic hydrolysis: adjust the pH of the slurry to 6.1; add a compound enzyme solution to the slurry, the enzyme addition amount is 1.5mKat / g, a...

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PUM

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Abstract

The invention discloses an extraction method of polysaccharide from sargassum fusiforme. The extraction method comprises the following steps: (1) pulping; (2) primary enzymolysis; (3) specific-microorganism fermentative degradation; (4) secondary enzymolysis; (5) filtering; (6) ethanol precipitation; (7) vacuum drying. The extraction method disclosed by the invention has the beneficial effects that the existing extraction method of polysaccharide is improved, firstly the method of primary enzymolysis is adopted to remove monosaccharide, disaccharide, oligosaccharide and alkaloid and the like;then specific microorganism is utilized for degradation to fully release the polysaccharide, and the enzymolysis condition of complex enzyme is optimized.

Description

technical field [0001] The invention relates to a method for extracting hijiki polysaccharides, belonging to the technical field of polysaccharide extraction. Background technique [0002] Sargassum fusiforme (Harv.) Setch is a plant of the genus Sargassum in Phaeophyta, also known as goat's milk, sea barley, etc. It is widely distributed, and it is distributed along the coasts of Japan and North Korea, and it is more distributed in the coastal areas of Zhejiang. In Japan, hijiki is called "longevity vegetable"; in my country, hijiki has a long history of being used as medicine, and "Shen Nong's Materia Medica" and "Compendium of Materia Medica" record its edible and medicinal value. Hijiki itself is rich in nutrients, of which carbohydrates account for a large proportion, endowing it with more biological functions and higher development value. Hijiki is widely used in dietary therapy such as lowering blood fat, antithrombotic, antitumor, eliminating brain fatigue, enhanci...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 苏来金徐仰丽郑晓杰林胜利叶剑李彦坡邹盈
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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