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A kind of x-pro structure-specific ACE inhibitory peptide and its preparation method

An inhibitory peptide, x-pro technology, applied in the field of biological peptide preparation, can solve the problems of low specificity, unfavorable structure-activity relationship of ACE inhibitory peptides, etc.

Active Publication Date: 2020-11-03
OCEAN UNIV OF CHINA
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  • Description
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  • Application Information

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Problems solved by technology

At present, protein resources are mainly hydrolyzed by proteases with relatively low specificity, such as neutral, alkaline, pepsin, and trypsin. There is a lot of randomness and blindness, which is not conducive to the study of the structure and activity of ACE inhibitory peptides. relationship, research on directional-specific enzymatic hydrolysis of proteins is rarely involved

Method used

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  • A kind of x-pro structure-specific ACE inhibitory peptide and its preparation method
  • A kind of x-pro structure-specific ACE inhibitory peptide and its preparation method
  • A kind of x-pro structure-specific ACE inhibitory peptide and its preparation method

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Embodiment 1

[0048] 1. Preparation of ACE inhibitory peptides

[0049] Preparation

[0050] Homogenize the whole oyster meat, add an appropriate amount of water, control the solid content of the raw material to 3%, add chymotrypsin accounting for 0.2%-2.0% of the solid content, and enzymolyze it at 37°C and pH 8.0 for 1-6h, Adjust the pH to 4.0, then add 50-1000U / g proline-specific endonuclease, enzymatically hydrolyze at 50°C for 1-6h, heat in a boiling water bath for 10min to inactivate the enzyme activity, and centrifuge at 8000rpm for 5-20min to obtain a Highly active oyster ACE inhibitory peptide mixture rich in Pro, Tyr, Phe and Trp carboxy-terminal.

[0051] 2. Separation and purification

[0052] (1) Sephadex G25 Gel Filtration Chromatography

[0053] Prepare a 1.6×60cm gel filtration chromatography column with Sephadex G25 filler, elute with deionized water at a flow rate of 0.2mL / min, detect the absorption value of 280nm wavelength, load the sample several times, collect the p...

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Abstract

The invention discloses X-Pro-structure specific-ACE inhibitory peptides and a preparation method thereof. The preparation method comprises the steps: performing enzymolysis on oyster protein by usinga complex enzyme of chymotrypsin and proline-specific endoprotease, performing separation and purification on the obtained oyster protein hydrolysate through Sephadex G25 gel filtration chromatography, SuperdexTM 30 Increase 10 / 300 GL gel filtration chromatography and reversed-phase high performance liquid chromatography so as to obtain high-purity fractions, and identifying the amino acid sequences of the ACE inhibitory peptides as Ser-Ala-Pro, Ala-Met-Pro, Thr-Ser-Gly-Pro and Ser-Asp-Pro. The four peptides are not reported, and a new way is provided for preparation of drugs for treatment ofhypertension.

Description

technical field [0001] The invention relates to the technical field of preparation of biological peptides, in particular to an X-Pro structure-specific ACE inhibitory peptide and a preparation method thereof. Background technique [0002] Hypertension is one of the most common cardiovascular diseases. It can cause damage to the brain, cardiovascular system, and kidneys. It is an important factor causing stroke, heart failure, and coronary heart disease, which seriously threatens human health. Therefore, the treatment and prevention of hypertension is of great significance to improving human health and prolonging life. Angiotensin-converting enzyme (ACE) regulates blood pressure in the human body through the renin-angiotensin system and the kallikrein-kinin system. ACE can convert angiotensin I to angiotensin II, making peripheral arterioles , vascular smooth muscle contraction, while stimulating the secretion of aldosterone, and promote the human kidney to Na + 、K + The r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083C07K5/103C07K1/20C07K1/16C12P21/06
CPCC07K5/0806C07K5/081C07K5/101C12P21/06
Inventor 牟海津张坦
Owner OCEAN UNIV OF CHINA
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