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Application of bletilla striata oligosaccharide and composition thereof in adjusting glucose and lipid metabolism disorder

A technology of Bletilla striata oligosaccharides and glucose and lipid metabolism, which can be used in metabolic diseases, drug combinations, medical preparations containing active ingredients, etc., and can solve problems such as diabetes that cannot be finally cured

Active Publication Date: 2019-02-22
HUBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drug targets mainly act on internal organs, and there are drug dependence and liver and kidney toxic side effects to varying degrees, and none of them can ultimately cure diabetes

Method used

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  • Application of bletilla striata oligosaccharide and composition thereof in adjusting glucose and lipid metabolism disorder
  • Application of bletilla striata oligosaccharide and composition thereof in adjusting glucose and lipid metabolism disorder
  • Application of bletilla striata oligosaccharide and composition thereof in adjusting glucose and lipid metabolism disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of Bletilla striata oligosaccharides by strong acid method

[0037] In this embodiment, a method for preparing bletilla striata oligosaccharides by strong acid hydrolysis is provided, and the specific implementation steps are as follows:

[0038] (1) Grind 25g of Bletilla striata tubers into 80 mesh powder, add 500ml of water to mix, dissolve and extract at 80°C for 8 hours to obtain an extract;

[0039](2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 50ml;

[0040] (3) Add 150 ml of 95% ethanol to the concentrated solution in step (2), and let stand overnight for 12 hours.

[0041] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;

[0042] (5) After the precipitate described in step (4) is redissolved, add trifluoroacetic acid (final concentration: 1.0M) ...

Embodiment 2

[0043] Example 2 Preparation of Bletilla striata oligosaccharides by enzymatic hydrolysis

[0044] In this embodiment, a method for preparing bletilla striata oligosaccharides by polysaccharide hydrolase is provided, and the specific implementation steps are as follows:

[0045] (1) Take 40g of Bletilla striata striata powder, add 600ml of water to mix, dissolve and extract at 40°C for 12 hours to obtain an extract;

[0046] (2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 60ml;

[0047] (3) Add 200 ml of 90% ethanol to the concentrated solution in step (2), and let stand overnight.

[0048] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;

[0049] (5) Redissolve the precipitate in step (4) with 50ml of water, add 1g of mannanase, hydrolyze at 40°C for 4 hours at pH 6.8, add a certai...

Embodiment example 1

[0052] Implementation case 1 Regulatory effect of bletilla striata oligosaccharides on obesity

[0053] In this example, the application of bletilla striata oligosaccharides to improve obesity is provided, and the specific implementation steps are as follows:

[0054] (1) Several C57BL / 6J mice were randomly divided into four groups (n>6): normal diet group (CD), high-fat diet group (HFD), normal diet combined with Bletilla striata oligosaccharide treatment group (CD- BO), high-fat diet combined with Bletilla striata oligosaccharide treatment group (HFD-BO).

[0055] (2) Bletilla striata oligosaccharides were administered by intragastric administration at a concentration of 100 mg / kg / d, and the duration of feeding and administration was 5 months.

[0056] (3) After the treatment, the body weight and blood lipid changes of the mice were detected, and the intestinal flora composition was analyzed by high-throughput sequencing.

[0057] (4) Dissect mice, collect adipose tissue, ...

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Abstract

The invention discloses an application of bletilla striata oligosaccharide and a composition thereof in adjusting glucose and lipid metabolism disorder. The degree of polymerization of bletilla striata oligosaccharide is 2-20 and the molecular weight range is 200-5000 Da. The bletilla striata oligosaccharide and the composition thereof play roles of inhibiting fat generation, reducing glucose concentration of blood and the like, can adjust glucose and lipid metabolism disorder, and plays a relatively good role of improving glucose intolerance caused by high calorie diets and diabetes caused byobesity and the like. The bletilla striata oligosaccharide and the composition thereof can be used for prevention and treatment of disease of glucose and lipid metabolism disorder and intestinal flora disturbance thereof.

Description

technical field [0001] The invention specifically relates to the application of bletilla striata oligosaccharide and its composition in regulating glucose and lipid metabolism disorder. Background technique [0002] Life in modern cities is becoming more and more fast. Too many high-calorie diets and less energy-consuming exercise have caused the number of urban residents to suffer from obesity, diabetes, insulin resistance, fatty liver and other disorders of glucose and lipid metabolism to increase year by year. According to the statistics of the National Health and Family Planning Commission, the dietary fat energy supply ratio of Chinese residents is as high as 30%. According to data released in 2015, the overweight rate of adults is greater than 30%, the obesity rate is higher than 12%, and the prevalence of diabetes is as high as 9.7%. , have a huge impact on the healthy life of the people. High-fat, high-calorie diet and lack of exercise are important causes of obesit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61P3/00A61P3/10A61P31/02A61P1/16
CPCA61K31/702A61P1/16A61P3/00A61P3/10A61P31/02
Inventor 刘洪涛郑军平杨化冰邹小娟孔明望张志刚夏慧陈会敏
Owner HUBEI UNIV OF CHINESE MEDICINE
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