Application of bletilla striata oligosaccharide and composition thereof in adjusting glucose and lipid metabolism disorder
A technology of Bletilla striata oligosaccharides and glucose and lipid metabolism, which can be used in metabolic diseases, drug combinations, medical preparations containing active ingredients, etc., and can solve problems such as diabetes that cannot be finally cured
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Embodiment 1
[0036] Example 1 Preparation of Bletilla striata oligosaccharides by strong acid method
[0037] In this embodiment, a method for preparing bletilla striata oligosaccharides by strong acid hydrolysis is provided, and the specific implementation steps are as follows:
[0038] (1) Grind 25g of Bletilla striata tubers into 80 mesh powder, add 500ml of water to mix, dissolve and extract at 80°C for 8 hours to obtain an extract;
[0039](2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 50ml;
[0040] (3) Add 150 ml of 95% ethanol to the concentrated solution in step (2), and let stand overnight for 12 hours.
[0041] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;
[0042] (5) After the precipitate described in step (4) is redissolved, add trifluoroacetic acid (final concentration: 1.0M) ...
Embodiment 2
[0043] Example 2 Preparation of Bletilla striata oligosaccharides by enzymatic hydrolysis
[0044] In this embodiment, a method for preparing bletilla striata oligosaccharides by polysaccharide hydrolase is provided, and the specific implementation steps are as follows:
[0045] (1) Take 40g of Bletilla striata striata powder, add 600ml of water to mix, dissolve and extract at 40°C for 12 hours to obtain an extract;
[0046] (2) Filter the extract described in step (1), obtain the filtrate after intercepting the precipitate, and distill under reduced pressure to obtain a concentrated solution of 60ml;
[0047] (3) Add 200 ml of 90% ethanol to the concentrated solution in step (2), and let stand overnight.
[0048] (4) Filter the concentrated solution after step (3) standing overnight, and obtain the precipitate after intercepting;
[0049] (5) Redissolve the precipitate in step (4) with 50ml of water, add 1g of mannanase, hydrolyze at 40°C for 4 hours at pH 6.8, add a certai...
Embodiment example 1
[0052] Implementation case 1 Regulatory effect of bletilla striata oligosaccharides on obesity
[0053] In this example, the application of bletilla striata oligosaccharides to improve obesity is provided, and the specific implementation steps are as follows:
[0054] (1) Several C57BL / 6J mice were randomly divided into four groups (n>6): normal diet group (CD), high-fat diet group (HFD), normal diet combined with Bletilla striata oligosaccharide treatment group (CD- BO), high-fat diet combined with Bletilla striata oligosaccharide treatment group (HFD-BO).
[0055] (2) Bletilla striata oligosaccharides were administered by intragastric administration at a concentration of 100 mg / kg / d, and the duration of feeding and administration was 5 months.
[0056] (3) After the treatment, the body weight and blood lipid changes of the mice were detected, and the intestinal flora composition was analyzed by high-throughput sequencing.
[0057] (4) Dissect mice, collect adipose tissue, ...
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