Culturing method of gerbera jamesonii novel variety
A cultivation method and technology for new varieties, applied in the directions of flower cultivation, botanical equipment and methods, culture medium, etc., can solve the problems of inability to maintain the excellent characteristics of the mother plant, affecting the promotion of new varieties, slow propagation of seedlings, etc. The effect of culture cycle, strong repeatability and good quality
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Embodiment 1
[0015] A cultivation method for a new variety of gerbera, comprising: explant callus induction, proliferation cultivation, rooting cultivation, seedling hardening and transplanting, specifically comprising the following steps:
[0016] 1) Callus induction of explants: take the lateral buds of a new variety of Gerbera chrysanthemum with strong growth, disinfect with 75% alcohol for 30 seconds, then disinfect with 0.1% mercury chloride solution for 5 minutes, rinse twice with sterile water, and take the stems The tip part was cut off, the length was 0.3mm, inserted into MS solid medium without exogenous hormones, and cultured under the conditions of temperature 22°C, light intensity 2500lx, and light time 24h / d to obtain callus tissue in vitro Under the action of suitable nutrients, hormones and other external conditions, the living plant cells in the state can show totipotency and develop into a complete plant. The induction of callus is to induce the cells in the differentiated...
Embodiment 2
[0021] A cultivation method of a new gerbera variety, specifically comprising the following steps:
[0022]1) Callus induction of explants: take the lateral buds of a new variety of Gerbera chrysanthemum with strong growth, disinfect them with 85% alcohol for 40 seconds, then disinfect them with 0.1% mercuric chloride solution for 10 minutes, rinse them twice with sterile water, and take the stems. The tip part was cut off, the length was 0.5mm, inserted into the MS solid medium without exogenous hormones, and cultured under the conditions of temperature 24°C, light intensity 2800lx, and light time 24h / d to obtain callus;
[0023] 2) Proliferation culture: insert the callus into the proliferation medium, and under the conditions of temperature 26°C, light intensity 1300lx, and light time 13h / d, cultivate new shoots to obtain clustered shoots after proliferation. Contains the following ingredients: MS+6-benzylaminoadenine 0.5mg / l+KT kinetin 0.3mg / l+naphthaleneacetic acid 0.2mg / ...
Embodiment 3
[0027] A cultivation method of a new gerbera variety, specifically comprising the following steps:
[0028] 1) Callus induction of explants: take the lateral buds of a new variety of Gerbera chrysanthemum with strong growth, disinfect with 80% alcohol for 35 seconds, then disinfect with 0.2% mercuric chloride solution for 8 minutes, finally rinse with sterile water for 3 times, and take the stems The tip part was cut off with a length of 0.4mm, inserted into MS solid medium without exogenous hormones, and cultured under the conditions of temperature 25°C, light intensity 3000lx, and light time 24h / d to obtain callus;
[0029] 2) Proliferation culture: insert the callus into the proliferation medium, and under the conditions of temperature 28°C, light intensity 1500lx, and light time 14h / d, cultivate new shoots to obtain clustered shoots after proliferation. Contains the following ingredients: MS+6-benzylaminoadenine 0.4mg / l+KT kinetin 0.3mg / l+naphthaleneacetic acid 0.2mg / l+aga...
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