Tissue culture technology for cottonrose hibiscus leaves

A tissue culture and hibiscus leaf technology, applied in the field of plant tissue culture, can solve the problems of unfavorable protection of high-quality germplasm resources, restricting the promotion and cultivation of high-quality hibiscus leaves, etc., and achieves the effect of accelerating popularization and resource development and utilization.

Inactive Publication Date: 2019-02-15
广西玉林市华睿茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hibiscus leaves are mainly propagated by seeds, but due to the separation of offspring traits of seed propagation, it is not conducive to the protection of high-quality germplasm resources, which restricts the popularization and planting of high-quality hibiscus leaves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 10 minutes, then rinse them with tap water for 12 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 2 seconds in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 15 minutes, wash with sterile water for 6 times, dry and cut into 2.5cm-long stems and inoculate them on the induction medium. Cultivate in complete darkness at 26°C for 16 days, then place in light for 12 hours a day, light intensity 1500x, and culture temperature 26°C for 41 days to induce the formation of clustered buds, and the induction rate is 70%. The induction medium is MS+5mg / L6-BA+1.0mg / L NAA+0.5mmol / L La(NO 3 ) 2 +3.5% sucrose+0.55% agar+0.15% activated carbon, the pH value is 5.8.

[0013] (2) Proliferation culture: Transfer the cluster...

Embodiment 2

[0017] (1) Induction of clustered buds: Select the budding stems of young trees that grow vigorously and have no diseases and insect pests in the current year as explants, soak them in an aqueous solution of detergent for 15 minutes, then rinse them with tap water for 18 minutes, dry the surface moisture and set aside. Soak in 75% ethanol for 16s in an ultra-clean workbench, sterilize with 0.1% mercuric chloride solution for 16min, wash with sterile water for 8 times, dry and cut into 2.6cm-long stems and inoculate them on the induction medium. Cultivate in total darkness at 27°C for 20 days, and then place in the light for 16 hours a day, the light intensity is 1600x, and the culture temperature is 27°C for 40 days to induce the formation of clustered buds, and the induction rate is 76%. The induction medium is MS+6mg / L6-BA+1.1mg / L NAA+0.5mmol / L La(NO 3 ) 2 +3.6% sucrose+0.5% agar+0.2% activated carbon, the pH value is 5.9.

[0018] (2) Proliferation culture: Transfer the c...

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PUM

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Abstract

The invention discloses a tissue culture technology for cottonrose hibiscus leaves. The cottonrose hibiscus leaf is dried leaf of hibiscus mutabilis, mainly produces in Zhejiang and Jiangsu, is prepared by harvesting in summer and autumn, removing impurities and airing or drying, faint in odor and slight in taste, has the effects of resolving toxins and dispersing swelling, cooling blood and reliving pain, and is applied to treating deep-rooted ulcer, sore and furuncle, venomous snake bites, mammary pain, traumatic injury and burn and scald. The tissue culture technology is a seedling railingmethod for obtaining high-quality cottonrose hibiscus leaf seedlings by an in-vitro rapid propagation technology. According to the tissue culture technology disclosed by the invention, stems with budsof the cottonrose hibiscus leaves are taken as explants, and are subjected to explant disinfection, induction of clustered shoots, rooting culture, seedling hardening and transplanting and the like,so that the tissue culture and rapid propagation system for the cottonrose hibiscus leaf is established. The tissue culture technology has vital practical significances for accelerating popularizationof improved varieties of the cottonrose hibiscus leaves and development and utilization of resources.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a tissue culture technique for hibiscus leaves. Background technique [0002] Hibiscus leaves are Malvaceae plants, the dry leaves of hibiscus hibiscus. It is mainly produced in Xijiang and Jiangsu. Harvest in summer and autumn, remove impurities, and dry or dry. Slight gas, light taste. Has detoxification and detumescence, cools blood, relieves pain. For gangrene furuncle, poisonous snake bites, mastalgia, bruises, burns. Hibiscus leaves are mainly propagated by seeds, but due to the separation of offspring traits of seed propagation, it is not conducive to the protection of high-quality germplasm resources, which restricts the popularization and planting of high-quality hibiscus leaves. Therefore, it is necessary to establish the tissue culture technology of hibiscus leaf to provide technical support and guarantee for the rapid propagation of its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈金水肖华洲
Owner 广西玉林市华睿茶业有限公司
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