A magnetic particle buffer for luteinizing hormone assay kit

A technology of luteinizing hormone and buffer solution, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems of HCG high crossover, cross-reaction interference, and uneven quality, so as to avoid instrument Air detection, not easy to settle, high specific effect

Active Publication Date: 2021-01-01
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main difficulty in LH detection is the cross-reaction interference of pituitary hormones such as HCG, FSH, and TSH
The quality of LH detection reagents on the market varies, and the existing products have the problem of high crossover to HCG. Therefore, it is urgent to develop new LH detection kits to avoid cross-reaction interference

Method used

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  • A magnetic particle buffer for luteinizing hormone assay kit
  • A magnetic particle buffer for luteinizing hormone assay kit
  • A magnetic particle buffer for luteinizing hormone assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A magnetic particle buffer solution used in the luteinizing hormone assay kit, the buffer solution is prepared according to Table 2. Wash and disperse the magnetic particles with the prepared magnetic particle buffer.

[0030] Table 2 is the magnetic particle buffer formula of embodiment 1

[0031] Antifoam SE-15 0.1mL / L trisodium citrate 0.24mol / L Basic buffer Set volume to 1L

Embodiment 2

[0033] A magnetic particle buffer solution used in the Luteinizing Hormone Assay Kit, the buffer solution is prepared according to Table 3. Wash and disperse the magnetic particles with the prepared magnetic particle buffer.

[0034] Table 3 is the magnetic particle buffer formula of embodiment 2

[0035] Antifoam SE-15 0.04mL / L trisodium citrate 0.35mol / L Basic buffer Set volume to 1L

Embodiment 3

[0037] A kind of magnetic particle buffer solution for luteinizing hormone assay kit, the buffer solution is prepared according to Table 4. Wash and disperse the magnetic particles with the prepared magnetic particle buffer.

[0038] Table 4 is the magnetic particle buffer formula of embodiment 3

[0039] Antifoam SE-15 0.57mL / L disodium citrate 0.06mol / L Basic buffer Set volume to 1L

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Abstract

The invention discloses magnetic-particle buffers used for a human luteinizing hormone (LH) determination kit. One of the magnetic particle buffers is formed by a base buffer, an antifoaming agent, and citrate. The antifoaming agent is added in 1L of the base buffer according to final concentration of 0.003% to 0.06% v / v, and the citrate is added according to final concentration of 0.06 to 0.35 mol / L. According to magnetic particles using the buffer, dispersibility is good under a natural gravity condition, foam is not liable to be generated in a mixing process, and detection specificity of immunomagnetic particles in the buffer is high.

Description

technical field [0001] The invention relates to the field of chemiluminescence immunoassay, in particular to a magnetic particle buffer used in a luteinizing hormone assay kit. Background technique [0002] Luteinizing Hormone (Human Luteinizing Hormone, LH) is a glycoprotein hormone synthesized and secreted by the anterior pituitary gland, consisting of two subunits, α and β. LH synthesis is regulated by sex hormone-releasing hormone (GnRH) in the hypothalamus, with feedback from steroid sex hormones. LH stimulates female ovulation and ovarian secretion of estrogen and progesterone; controls male testicular Leydig cells to secrete testosterone. In women, LH content is low during the follicular phase; it peaks in the middle of menstruation, triggering ovulation, and drops to a minimum after ovulation. LH is highest in postmenopausal women, even higher than the midmenstrual LH peak; LH levels are also high in gonadically ablated men. LH is important for the analysis of gon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 胡容张凌燕席强田君喜
Owner SICHUAN MACCURA BIOTECH CO LTD
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