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Breeding of functional biocontrol bacterial fertilizer microorganisms and preparation of bacterial fertilizer series products

A product and technology of bacterial fertilizer, applied in the field of microbial compound fertilizer and its preparation, can solve problems such as failure to use, straw solid waste pollution, environmental pollution and the like

Inactive Publication Date: 2019-02-05
天津市天大天福生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is also solid waste pollution such as straw. Our country produces about 6.5×1011kg of crop straw every year, 40% of which are not utilized, causing environmental pollution.

Method used

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  • Breeding of functional biocontrol bacterial fertilizer microorganisms and preparation of bacterial fertilizer series products
  • Breeding of functional biocontrol bacterial fertilizer microorganisms and preparation of bacterial fertilizer series products
  • Breeding of functional biocontrol bacterial fertilizer microorganisms and preparation of bacterial fertilizer series products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of basic "composite flora":

[0048] The present invention takes the fertile black soil from the rhizosphere humus soil under the beauty pine trees of 352 ancient pine trees of more than one hundred years old, and carries out the separation and screening of microbial strains, so as to select the beneficial plant growth microorganisms; Separation and screening of species in order to breed beneficial plant growth microorganisms; from the fertile black soil from the reed rhizosphere soil of Aiwan Lake in Tianjin University, which has grown strong reeds for many years, for the separation and screening of microbial strains in order to select and breed beneficial plants that degrade environmental pollution Growing microorganisms (see references for isolation and screening methods:. Production and application effects of Azotobacter chroococcus and Bacillus megaterium compound fertilizer[J]. Anhui Agricultural Sciences, 2010, 38(28): 15705-15706; Selecting Ba...

Embodiment 2

[0051] Example 2: Isolation and identification of major bacteria

[0052] Dilute the "composite flora" with "pesticide residue medium": straw powder 0.1-100g / L, broth (light), agar 1g / L, potato 10-200g / L, sucrose 1-30g / L , Soy flour 1-15g / L, Ammonium sulfate 0.1-5g / L, Dipotassium hydrogen phosphate 0.1-5g / L, Yeast powder 0.1-6g / L, PH5-8.5 form a medium, after sterilization, spread into a plate , To obtain a monoclonal strain.

[0053] 121 strains were isolated in the experiment, including 69 spore bacteria, 20 anaerobic spore bacteria, 10 lactic acid bacteria, 5 yeasts, 3 Pediococcus, 4 Pseudomonas, 4 actinomycetes, and 6 molds .

[0054] It was amplified by 16SrDNA universal primers and sent to a sequencing company for sequencing, and the strains in Table 1 were identified.

[0055] Table 1 Strain purification, screening and identification results

[0056]

Embodiment 3

[0057] Example 3: The carrier form of bacterial fertilizer and the preparation of bacterial fertilizer in different states

[0058] The bacterial fertilizer carrier of the present invention has three forms, which are determined according to customer needs:

[0059] (1) The microorganism is in a dormant state. The carrier: This form is easy to store. Microbial spores or fossil microorganisms are mixed with dolomite or calcium carbonate or shell powder. The effective viable count is lower than the effective viable count of liquid microbial fertilizer. The shortcomings of microorganisms need to be activated. , And most of the space is occupied by dolomite or calcium carbonate, which requires irrigation and rain to release microorganisms, which may affect the effect of fertilizer use.

[0060] (2) Dry or gelatinous starch fertilizer, most of the bio-fertilizers available on the market now exist in the form of starch. Microorganisms will stay in a dormant state for a certain period of ti...

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Abstract

The invention discloses a preparation method of a bacterial fertilizer or a bacterial product. The preparation method is characterized by comprising the following steps: uniformly mixing a type A microbial agent and a type B microbial agent in the ratio of 1 to (0.1 to 10); adding the uniformly-mixed microbial agent into a pesticide residue culture medium to obtain the bacterial fertilizer or thebacterial product. Active components of the microbial agent consist of rhizobium japonicum, bacillus megaterium, bacillus cereus, bacillus subtilis, bacillus mucilaginosus, lactobacillus buchneri, bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus pumillus, bacillus licheniformis, trichoderma harzianum, etc. The microbial agent and pesticide residues are mixed uniformly to obtain the bacterial fertilizer or the bacterial product. The bacterial fertilizer or the bacterial product can be applied to the facilitation of crop growth or the improvement of crop yield or the improvement of planting soil, as well as the improvement of crop disease resistance. The product can be combined with different bacterial fertilizer carriers to form a solid bacterial fertilizer, a dry or gel starchbacterial fertilizer, a liquid bacterial fertilizer, a pesticide residue waste bacterial fertilizer, a grass charcoal bacterial fertilizer, a peat bacterial fertilizer, a hibernated bacterial fertilizer, as well as a pig, cattle, sheep and chicken manure excrement bacterial fertilizer, and to form seed coatings.

Description

Technical field [0001] The invention relates to a microbial compound fertilizer and a preparation method thereof, belonging to applied microbial technology and fertilizer manufacturing technology. Background technique [0002] Bacterial fertilizer is also known as biological fertilizer, biological fertilizer, bacterial fertilizer or inoculant, etc. It contains a large number of microorganisms. Through the life activities of microorganisms in the soil, it improves the nutritional conditions of crops and participates in the fixation of nitrogen in the soil and the transformation of substances and energy. , The formation and decomposition of humus, the release of nutrients. It is an artificial way to increase the number of beneficial microorganisms in the soil, which can enhance the activity of the microorganisms in the soil, thereby improving the fertility of the soil. [0003] Biological hormones stimulate crop growth. The microorganisms in the polyspore fertilizer will produce a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F17/00C09K17/14A01N63/00A01P21/00C09K101/00
CPCA01N63/00C05B7/00C05F11/00C05F17/00C05G3/00C09K17/14C09K2101/00C05G5/20C05F11/08C05C3/00Y02W30/40
Inventor 王昱蓉季静王罡
Owner 天津市天大天福生物技术有限公司
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