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A kind of human tissue factor coagulation complex and its preparation method

A tissue factor and complex technology, applied in the field of human tissue factor coagulation complex, can solve the problems of lack of cytokine secretion and platelet recruitment, single function of thrombin, etc., to achieve high hemostasis efficiency, good human compatibility, Full-featured effects

Active Publication Date: 2021-05-07
厦门宏谱福生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Active local hemostatic materials have the advantage of rapid onset of effect. For extensive wound bleeding, local spraying can be used for hemostasis without compression and packing. However, the function of thrombin is single, there is no cascade amplification effect of coagulation factors, and the necessary cells in the coagulation pathway are lacking. Role of factor secretion and platelet recruitment

Method used

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  • A kind of human tissue factor coagulation complex and its preparation method
  • A kind of human tissue factor coagulation complex and its preparation method
  • A kind of human tissue factor coagulation complex and its preparation method

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Embodiment 1

[0052] (1) Cell membrane localization detection of tTF-PHLIP fusion protein in cells: HEK293T cells stably expressing tTF-PHLIP fusion protein were cultured in a confocal dish with DMEM medium containing 10% fetal bovine serum and appropriate amount of antibiotics, When the cells adhere to the wall and the confluence reaches about 50%, aspirate the culture medium, wash it carefully with PBS, fix the cells with 4% paraformaldehyde for 15 minutes, soak in PBS for 3 times; permeabilize with 0.5% Triton X-100 at room temperature for 20 minutes, Soak and wash 3 times; drop a sufficient amount of diluted rabbit-derived anti-tissue factor primary antibody, incubate at 37°C for 1 hour, soak and wash 3 times with PBS; drop a sufficient amount of diluted fluorescence-conjugated anti-rabbit antibody secondary antibody , incubate at 37°C for 1 hour, soak in PBS for 3 times; add DAPI (4',6-diamidino-2-phenylindole dihydrochloride) dropwise and incubate in the dark for 5 minutes, stain the n...

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Abstract

The invention discloses a human tissue factor coagulation complex and a preparation method thereof. The complex has the function of rapid local hemostasis, prevents wound infection, promotes wound healing, etc., and has a compound effect different from that of traditional local coagulants. The system of the present invention is simple to prepare, and is applicable to rapid hemostasis of epidermis or tissue surface wounds caused by scattered or local, known or unknown bleeding points and trauma, antibacterial and wound healing, and can be used in liver injury, articular cartilage injury, eye It is more advantageous in the application of injury, diabetes, hemophilia and other conditions.

Description

technical field [0001] The invention relates to a human tissue factor coagulation complex. Background technique [0002] Bleeding is a very common situation in people's daily life and medical procedures, especially in surgery. Whether intraoperative hemostasis is fast and effective directly affects the prognosis of patients. The bleeding problem in the medical treatment of special patients such as diabetes and hemophilia is particularly difficult, so it is very important It is necessary to develop more effective hemostatic drugs with more diverse functions. Hemostatic strategies for intraoperative bleeding mainly include mechanical hemostasis, systemic application of hemostatic drugs, and local hemostatic drugs. [0003] Mechanical hemostasis is the most widely used clinical method and the simplest method, mainly including ligation, suture, electrocoagulation, packing and other methods, but in addition to gauze packing, the bleeding point must be clear before operation, for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/867A61K38/36A61P7/04
CPCA61K38/00A61P7/04C07K14/745C07K2319/00C12N15/86C12N2740/15043
Inventor 刘刚李文岗苗逢霖林惠荣
Owner 厦门宏谱福生物科技有限公司
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