Fully automatic micro-droplet emulsion generating device and generating method

Abstract

The invention provides a fully automatic micro-droplet emulsion generating device and generating method, wherein the device includes a base and a support plate; a slide rail is arranged on the base, amicro-droplet generating chip is arranged on the slide rail through a chip fixture frame, one side of the chip fixture is connected with a driving motor, and the driving motor moves to drive the chipfixture to move on the slide rail; one side of the support plate is provided with a guide rail, and an air pressure control module is arranged on the guide rail; an air cylinder is arranged above theguide rail, and an air cylinder shaft of the air cylinder is connected with a connection plate; the air cylinder moves, and the air pressure control module is driven to move on the guide rail. The reaction conditions of droplet formation can be adjusted by automatic configuration, so that micro-droplets with different sizes are produced, and the needs of different application occasions are met; sample pollution is isolated by a sealing cover, and a microdroplet generation chip is protected from being damaged by a press plate during operation. The eight-channel isolation system realizes preparation of droplets in two or multiple channels, is more convenient than conventional equipment and saves the use amount of reagents.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and in particular relates to a fully automatic micro-droplet emulsion generating device and a generating method thereof. Background technique [0002] Micro-emulsions formed from oil-water phases have been used in high-throughput analysis systems, and their application range has a tendency to expand. For example, emulsion formation is used in combination with polymerase chain reaction (PCR) to amplify a single copy of a nucleic acid target molecule in a droplet, thereby realizing the detection of the presence of a single molecule target and its gene sequence. 10X Genomics, an American company, uses the emulsion PCR method combined with GemCode technology to generate long-chain DNA, thereby realizing long-chain DNA sequence sequencing, or single-cell sequencing, which has been recognized by the industry as one of the best technologies for long-chain DNA sequencing and single-cell sequencing ...

AI Technical Summary

Problems solved by technology

At the same time, BioRad's emulsion generation microfluidic chip has 8 channels (emulsion preparation of 8 samples can be performed at the same time), but their system must do 8 channels at the same time. If the user has less than 8 samples, The user must fill the remaining channels with oil or buffer, resulting in wasted channel use

If the wrong sample volume is added to one of the 8 wells, the droplet generation in 8 wells will stop at the same time, resulting in sample loss

In addition, the user cannot know the specific reason for the droplet generation failure during the use of the instrument, and can only discard the droplet generation chip and the reagents used, and run again

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