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Polysaccharide modified MBG scaffold, tissue repair scaffold, and preparation method and application thereof

A tissue repair and polysaccharide technology, used in tissue regeneration, medical science, prosthesis, etc., can solve the problems of poor application of bone repair materials, excessive toxic and side effects, insufficient stability, etc., and achieve stable and long-acting slow-release growth factors. , good biocompatibility, high loading effect

Inactive Publication Date: 2019-01-29
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the growth factor itself is easily affected by internal and external conditions (such as body fluids, temperature, pH environment, and organic reagents, etc.), it has the following problems in the application process: ①The half-life is short, and it is easy to dissolve due to dilution, metabolism, and enzymatic hydrolysis. Inactivation; ② Potential excessive toxic side effects; ③ Expensive, taking rhBMP-2 produced by Medtronic in the United States as an example, the price of rhBMP-2 is as high as 12,000 yuan / mg
However, these methods still cannot achieve long-term sustained release while maintaining the activity of growth factors well, and at the same time have low loading efficiency and insufficient stability, and most of them are not very applicable to bone repair materials

Method used

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  • Polysaccharide modified MBG scaffold, tissue repair scaffold, and preparation method and application thereof
  • Polysaccharide modified MBG scaffold, tissue repair scaffold, and preparation method and application thereof
  • Polysaccharide modified MBG scaffold, tissue repair scaffold, and preparation method and application thereof

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preparation example Construction

[0101] The above-mentioned preparation method of the MBG stent has a simple technological process and can be operated on a large scale industrially. By adjusting the polyurethane sponge template, MBG stents with different shapes and structures can be obtained to meet different actual needs. The MBG scaffold obtained by the method has a uniform structure and a stable microscopic shape, which is beneficial to the operation of the amino group and / or polysaccharide modification process in the later stage.

[0102] In a preferred embodiment of the present invention, according to the actual needs and requirements of tissue repair, the required template can be prepared by 3D printing, so as to prepare the required MBG scaffold, or the MBG scaffold can be prepared directly by 3D printing .

[0103] In a preferred embodiment of the present invention, the preparation of MBG sol comprises the following steps:

[0104] (a) Add 300mL of absolute ethanol and 6g of 0.5M HCl to the reaction ...

Embodiment 1

[0130] The preparation of embodiment 1 mesoporous active glass (MBG)

[0131] The flow chart of the preparation steps of mesoporous active glass (MBG) is as follows: figure 1 shown. Specific steps are as follows:

[0132] (a) Add 300mL of absolute ethanol and 6g of 0.5M HCl to the reaction vessel in a 30°C water bath;

[0133] (b) Add 24g F127 (EO 106 PO 70 EO 106 ) vigorously stirred until completely dissolved, and the solution was in a clear state;

[0134] (c) Add 6.56g calcium nitrate tetrahydrate (Ca(NO 3 ) 2 4H 2 0), stirred for 10min until it was completely dissolved;

[0135] (d) adding 1.38 g of triethyl phosphate (TEP);

[0136] (e) Add 31.2 g tetraethyl orthosilicate (TEOS) dropwise;

[0137] (f) After the addition of the reagents, keep stirring vigorously in a water bath at 30°C for 24 hours.

Embodiment 2

[0138] Example 2 Preparation of MBG scaffold

[0139] The flow chart of the preparation steps of MBG scaffold is as follows: figure 2 shown. Specific steps are as follows:

[0140] (a) Using a polyurethane sponge as a macroporous template. After the polyurethane sponge is processed into the corresponding specifications, it is soaked in 20% NaOH solution and rubbed for 2 hours for pretreatment;

[0141] (b) Rotate the prepared MBG sol on a rotary evaporator at 60°C for 30 min to obtain a viscosity of 4×10 4 -6×10 4 mPa·s viscous sol;

[0142] (c) partially drying the sol prepared in (b), ball milling and sieving to obtain MBG microspheres with a particle size of about 10 μm;

[0143] (d) The MBG microspheres prepared in (c) and the micropore pore-forming agent (polyacrylic acid microspheres, 20 μm) were uniformly mixed at a mass ratio of 1:1:6 (MBG microspheres: polyacrylic acid microspheres: MBG sol) Mixed into (b) the MBG sol after rotary evaporation treatment, and wi...

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Abstract

The invention relates to the field of a biological medicine material, and concretely provides a polysaccharide modified MBG scaffold, a tissue repair scaffold, and a preparation method and applicationthereof. The polysaccharide modified MBG scaffold provided by the invention comprises an MBG scaffold and a polysaccharide derivative loaded on the MBG scaffold, wherein the MBG scaffold has excellent biological activity and structure features; the polysaccharide derivative is used for performing polysaccharide modification on MBG; the obtained polysaccharide modified MBG scaffold can delay the release speed of growth factors in the material; and the high-activity release of the growth factors can be realized. The tissue repair scaffold, the preparation method and the application provided bythe invention achieve the advantage of the polysaccharide modified MBG scaffold; and the effects of slowly releasing the growth factors and repairing tissues can be achieved.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a polysaccharide-modified MBG scaffold, a tissue repair scaffold and a preparation method and application thereof. Background technique [0002] Bone tissue defect and injury has become an important disease affecting people's health and life. In order to overcome the problems of limited bone harvesting source, secondary surgery pain, immune rejection and potential disease transmission caused by autologous / allograft bone transplantation, the development of artificial bone repair materials has become a research hotspot in recent years. However, the artificial bone repair materials currently used clinically are quite limited, mainly including calcium phosphate (such as tricalcium phosphate, porous hydroxyapatite bioceramics and calcium phosphate bone cement, etc.), biological glass-ceramics and collagen and other types of products. Although these materials can guide bone tissue ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/22A61L27/56A61L27/50A61L27/10A61L27/20
CPCA61L27/10A61L27/20A61L27/227A61L27/50A61L27/54A61L27/56A61L2300/252A61L2300/414A61L2300/602A61L2430/02C08L5/08C08L5/10
Inventor 唐为王国成
Owner SHENZHEN INST OF ADVANCED TECH
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