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Use of mimosa pudica extracts for manufacture of composition for inhibiting mmp2 gene expression and collagen degradation

A technology of collagen and gene expression, applied in the direction of drug combinations, plant raw materials, cosmetic preparations, etc., can solve the problems of limited skin collagen synthesis and inability to effectively supplement collagen

Active Publication Date: 2019-01-29
百岳特美肤生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the barrier effect of the epidermis of the skin, applying skin care products containing collagen directly on the surface of the skin cannot effectively replenish the collagen in the deep layer of the skin
On the other hand, if collagen is ingested orally, since macromolecular collagen is decomposed into small molecular amino acids in the digestive tract, after obtaining these amino acids in various parts of the human body, the required collagen may not be synthesized, so oral collagen To enhance skin collagen synthesis has limited effect

Method used

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  • Use of mimosa pudica extracts for manufacture of composition for inhibiting mmp2 gene expression and collagen degradation
  • Use of mimosa pudica extracts for manufacture of composition for inhibiting mmp2 gene expression and collagen degradation
  • Use of mimosa pudica extracts for manufacture of composition for inhibiting mmp2 gene expression and collagen degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of Herba Malawi Extract

[0028] Firstly, wash and dry the Herba Aphrodisiac, and grind it with a homogenizer. Secondly, water, alcohols, or alcohol-water mixtures are used as solvents to extract the homogeneous herba cuspidatum, and the liquid-solid ratio of the mixture of the solvent and the homogeneous herba matura is 5-20:1-5. The extraction temperature is between 50°C-100°C, preferably 75°C-95°C. The extraction time in this embodiment is 0.5-2 hours.

[0029] After cooling down to room temperature, the Herba Malawi extract obtained through the above extraction steps can be filtered through a 400-mesh filter to remove residual solids. The filtered Kumarinaceae extract can be further concentrated under reduced pressure at 45° C. to 70° C. to obtain a concentrated product. In order to obtain the solid extract of Herba cuspidatum, the extract of Herba cuspidatum concentrated under reduced pressure can be spray-dried to remove the solvent, so as to obtain...

Embodiment 2

[0031] Matrimony pratense extract enhances collagen production in fibroblasts

[0032] In order to test the effect of the extract of Coyote husbandry of the present invention on the collagen production of skin cells, the present embodiment uses the collagen content test to analyze the collagen production of human skin fibroblasts CCD-966SK after being treated with the water extract of Coppinwort. Quantitative changes. First, human dermal fibroblasts were seeded at 2×104 cells / well in each well of a 24-well dish containing 500 μL of cell culture medium. After culturing at 37°C for 24 hours, the cell culture medium was removed and the cells were washed with PBS solution. . Next, 500 μL, 1 mg / mL water extract of Herba Kumarin and 500 μL of FBS-free cell culture medium were added to the cells in each well, or cells were only treated with 500 μL of FBS-free cell culture medium as a control group. After the aforementioned two groups of cells were cultured at 37° C. for 48 hours, t...

Embodiment 3

[0035] Collagen officinalis extract inhibits the expression of genes related to collagen breakdown

[0036] In order to further explore the gene expression regulation effect of the extracts of the husbandry grass of the present invention on the gene expression of collagen decomposing enzymes, the present embodiment uses Q-PCR to measure the human dermal fibroblasts CCD-966SK (BCRC 60153) treated with the water extract of the husbandry grass, Changes in the expression of genes related to collagen breakdown. First, human dermal fibroblasts were seeded at 1.5×105 cells / well in each well of a 6-well plate containing 2 mL of cell culture medium, and cultured at 37°C. Next, the cells were treated with 0.5 mg / mL or 1 mg / mL of water extract of Herba Kumarin for 24 hours, and then the treated cells were collected for Q-PCR. The human dermal fibroblasts in the control group were treated with the cell culture medium without water extract of Kumarinaceae.

[0037] The relative expressio...

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Abstract

The present invention relates to the use of a mixture of husband and wife grasses, and more particularly to the use of a mixture of husband and wife grass extracts for the preparation of a compositionfor inhibiting matrix metalloproteinase 2 (MMP2) gene expression and collagen breakdown. The husband and wife grass extract of the present invention is obtained by extracting the couple grass with water, an alcohol or an alcohol-water mixture as a solvent. The couple's grass extract reduces the decomposition of collagen in the skin by inhibiting the gene expression of MMP2 in the skin fibroblasts, thereby contributing to skin elasticity and firmness, reducing fine lines and wrinkles, and delaying skin aging.

Description

technical field [0001] The present invention relates to the use of an extract of Kumarin, in particular to the use of the extract of Kumarin for preparing a composition for inhibiting matrix metalloproteinase 2 (MMP2) gene expression and collagen decomposition. Background technique [0002] Collagen is a highly abundant protein in animals, accounting for about 25% of the total protein in the body. Collagen is mainly distributed in the extracellular matrix of connective tissues in animals, such as ligaments, tendons, cartilage, and eye corneas. It is the most important protein in connective tissues, accounting for about 20% to 30% of connective tissues. Collagen has a fibrous structure that can provide the mechanical support and strength required by connective tissue. It is also related to blood coagulation, wound healing, and tissue repair. It is a very important structural protein. Collagen in the human body can be divided into different types due to differences in the com...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P39/06A61P17/00A23L2/38A23L33/105
CPCA61P17/00A61P39/06A23L2/38A23L33/105A61K36/48A23V2002/00A61K2236/333A61K2236/33A61K2236/331A23V2200/30A23V2250/21A61K8/9789A61Q19/08
Inventor 林咏翔林于婷
Owner 百岳特美肤生物技术(上海)有限公司
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