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Method for preparing composite carrier by taking modified carbon fiber and polyurethane as raw materials

A composite carrier, polyurethane technology, applied in the direction of being fixed on or in an inorganic carrier, immobilized on/in an organic carrier, etc., can solve the problems in the field of immobilization of few cells, and achieve a favorable immobilization of cells. And multi-batch use, good biocompatibility, improved biocompatibility

Active Publication Date: 2019-01-25
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Polyurethane and carbon fiber composites prepared by existing technologies have increasingly shown advantages, but their main applications are in material engineering, and rarely used in the field of cell immobilization

Method used

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  • Method for preparing composite carrier by taking modified carbon fiber and polyurethane as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of carrier: Add 1g of carbon fiber powder to 200mL, 4% terephthalic acid solution for coating treatment, then add 1g of 0.5cm*0.5cm*0.5cm through 3-methyl-2-oxirane After fully soaking and mixing the methanol-modified polyurethane, ultrasonically disperse it evenly for 24 minutes, and dry it in a blast drying oven for 32-48 minutes. The far-infrared radiator is irradiated for 30 minutes, and the distance is 5-8 cm, and the composite carrier of polyurethane and carbon fiber is obtained.

[0026] Multi-batch fermentation of immobilized cells: Micrococcus luteus was activated and cultured in the seed medium for 20 hours at 30°C, and inoculated with a 6% inoculation amount in the fermentation medium containing a certain amount of carrier, and cultivated for 36 hours. The resulting product is determined by HPLC method, the condition is: chromatographic column: Sugar-Pak TM  1 6.5*300mm column, mobile phase: ultrapure water, flow rate: 1mL / min, temperature: 80°C....

Embodiment 2

[0029] Preparation of the carrier: Add 1.5g of carbon fiber powder to 120mL, 6.3% hexanediol solution for coating treatment, then add 1g of polyurethane modified with glycidol with a size of 0.6cm*0.6cm*0.6cm, fully After soaking, ultrasonically disperse it for 27 minutes to make it evenly dispersed, and dry it in a blast drying oven for 32-48 minutes. 10-1Pa, react in a low-temperature plasma generator with a power of 500W for 35min to obtain a composite carrier of polyurethane and carbon fiber.

[0030] Multi-batch fermentation of immobilized cells: Inoculate α-hemolytic streptococci into the seed medium at an inoculation amount of 2%, culture at 37°C, activate twice, and inoculate the bacterial suspension cultivated for 24 hours into the fermentation medium Among them, the inoculum amount was 4%, and the strain was fermented in multiple batches using a two-stage method. The obtained product was determined by the naphthol-sulfuric acid method, and the detection wavelength w...

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Abstract

Carbon fiber was modified by coating and polyurethane was hydrophilically modified to prepare composite carrier for immobilized cells. The composite carrier of modified carbon fiber and polyurethane is obtained by weighing certain quality carbon fiber powder, coating in certain concentration solution, adding certain quality epoxy alcohol modified polyurethane, fully immersing, drying initially, and radiation oxidation in air. Carboxyl groups were introduced into the surface of coated carbon fibers, and hydroxyl groups were introduced into epoxy alcohol modified polyurethanes. The modificationof the two materials not only played an oxidative role, but also radiation oxidation treatment was helpful to improve the problem that the cells with larger pore size of polyurethane were easy to falloff and the cells were easy to fall off from carbon fibers under high shear stress. The modified carbon fiber and the polyurethane composite carrier prepared by the invention has the characteristicsof simple operation, strong stability, good biocompatibility, good immobilization effect and the like, and is a good cell immobilization carrier.

Description

technical field [0001] The invention relates to the technical field of composite materials, in particular to a method for preparing a composite carrier by using modified carbon fiber and polyurethane as raw materials. technical background [0002] Polyurethane is a polymer material, the main chain contains -NHCOO- repeating structural units, it has a series of excellent properties such as high mechanical strength, chemical inertness, high wear resistance, low temperature performance, good bonding performance and good elasticity. Because it is widely used in light industry, chemical industry, textile, construction, aviation and other fields, polyurethane is known as "the fifth largest plastic". At present, due to the good compatibility of polyurethane, it has been used in the field of cell immobilization. However, in practical applications, due to the large pore size of polyurethane, there are disadvantages such as easy detachment of fixed cells. [0003] Carbon fiber is a m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08
CPCC12N11/08C12N11/14
Inventor 王乐沙宇韩肖瑶位启先
Owner HENAN UNIVERSITY OF TECHNOLOGY
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