Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A vaccine strain rsha-△200 and its construction method and application

A construction method and vaccine strain technology, applied in the field of vaccine strain rSHA-△200 and its construction, can solve problems such as virus mutation

Active Publication Date: 2021-01-12
YANGZHOU UNIV +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of continuous mutation of the existing H9 subtype AIV virus, the present invention provides a vaccine strain rSHA-△200 with strong immunity and good cross-immunity of H9 subtype AIV

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A vaccine strain rsha-△200 and its construction method and application
  • A vaccine strain rsha-△200 and its construction method and application
  • A vaccine strain rsha-△200 and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] 1. Hemagglutination test to screen candidate vaccine strains

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a vaccine strain rSHA-△200, its construction method and application, and relates to the field of influenza vaccines. The vaccine strain rSHA-Δ200 includes an HA gene that does not contain an NRT glycosylation site. The present invention finds out potential glycosylation sites on the HA protein through analysis, and deletes glycosylation sites through site-directed mutagenesis technology to construct HA expression plasmids with deletion of multiple glycosylation sites, and utilizes 8 plasmids to reverse The recombinant vaccine strain was obtained by genetic technology, and the antiserum was prepared. Through comparison, it was found that the rSHA-△200 obtained by the present invention has good cross-immunity and serum cross-neutralization ability, and is an ideal H9 subtype AIV inactivated vaccine candidate strain.

Description

technical field [0001] The invention relates to the technical field of influenza vaccines, in particular to a vaccine strain rSHA-Δ200 and its construction method and application. Background technique [0002] H9 subtype AIV is usually low-pathogenic avian influenza, which causes low poultry mortality. However, due to its wide distribution and fast transmission speed, the egg production of laying hens will drop sharply after infection, and broiler chickens will grow slowly. Reducing the feed-to-meat ratio has caused huge economic losses to the poultry farming industry. H9 subtype AIV infection is likely to lead to a sharp decline in the poultry's own immunity, resulting in concurrent or secondary infections of other bacteria or viruses leading to increased mortality. The H9 subtype AIV will also serve as the donor of the internal genes of other subtype influenza viruses, providing conditions for the generation of new influenza virus strains, triggering the outbreak of new i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/85A61K39/295A61K39/145A61P31/16C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70A61P31/16C12N7/00C12N15/85C12N2760/16121C12N2760/16134C12N2760/16152
Inventor 彭大新石宝兰陈素娟张伟秦涛王泽源乔依漪苏湘李卓恬沈海峰刘雷
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com