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Application of Trichoderma harzianum LTR-2

In one aspect, the technology of tobacco mosaic virus, applied in the field of microorganisms, can solve problems such as environmental damage and potential risks in the balance of the plant's own microbial community, and achieve the effects of easy operation, simple preparation method, and good control effect

Inactive Publication Date: 2019-01-25
ECOLOGY INST SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of Trichoderma harzianum LTR-2 for the prevalence of tobacco mosaic virus disease and the destruction of the environment by chemical pesticides, and the potential risk of the use of mixed bacterial agents on the balance of the plant's own microbial community. The application of the prevention and treatment of tobacco mosaic virus disease, more specifically the application of Trichoderma harzianum LTR-2 metabolites in the prevention and treatment of tobacco mosaic virus disease

Method used

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  • Application of Trichoderma harzianum LTR-2
  • Application of Trichoderma harzianum LTR-2
  • Application of Trichoderma harzianum LTR-2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Trichoderma harzianum Trichoderma harzianum The preparation method of LTR-2 metabolite is carried out by the following steps:

[0021] (1) Activation culture of strains: the separated and preserved Trichoderma harzianum Trichoderma harzianum Strain LTR-2 was inoculated on solid PDA medium, and the plate cultured at 30°C±1°C was covered with spores for inoculation;

[0022] (2) Obtaining of fermentation broth: Fermentation broth contains 200g of peeled potatoes per 1000mL, heated and boiled for 20min, filtered through 8 layers of gauze to remove filter residue, added water to the filtrate to 1000mL; added 20g of glucose, 20g of peptone, and yeast powder to each 1000mL of filtrate 5 g, potassium dihydrogen phosphate 3.0 g, magnesium sulfate heptahydrate 1.50 g, pH 7.0-7.5; 500 mL Erlenmeyer bottle with 100 mL liquid medium; Put the spores of Trichoderma harzianum LTR-2 into the liquid medium, and ferment for 120 hours at 30°C ± 1°C with a shaker speed of 160r / min;

[...

Embodiment 2

[0027] The inhibitory effect of Trichoderma harzianum LTR-2 metabolites on tobacco mosaic virus is carried out by the following steps:

[0028] (1) Preparation of mother liquor (0.5% metabolite of Trichoderma harzianum LTR-2): Accurately weigh 0.50 g of metabolite of Trichoderma harzianum LTR-2, add 100 mL of sterile water, fully dissolve by ultrasonic vibration, and finally prepare the concentration The mother liquor is 5000mg / L, that is, the mother liquor is 0.5% Trichoderma harzianum LTR-2 metabolite;

[0029] (2) Preparation of sample solution: Dilute the above mother solution with sterile water to 10×, 100× and 500× (concentrations are 500mg / L, 50mg / L and 10mg / L respectively);

[0030] (3) Determination of anti-virus activity: the resistance of metabolites to tobacco mosaic virus (TMV) was determined by half-leaf blight method. Choose vigorous and healthy Sansheng Tobacco at the true leaf stage, inoculate 100 μL of metabolites containing TMV and a small amount of silica ...

Embodiment 3

[0036] Embodiment 3 (comparison of product of the present invention and field common pesticide)

[0037] The implementation steps are implemented with reference to (3) in Example 2. The test results are shown in Table 2.

[0038] Table 2 Comparison of metabolites of Trichoderma harzianum LTR-2 and common pesticides

[0039]

[0040] It can be seen from Table 2 that the inhibitory effect of Trichoderma harzianum LTR-2 metabolites is similar to or slightly better than that of common chemical agents, and has high safety.

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Abstract

The invention relates to the technical field of microorganisms, in particular to a method for preparing a metabolite for controlling tobacco mosaic virus disease and its application. The invention provides a Trichoderma harzianum LTR-2 to control tobacco mosaic virus disease, more specifically application of Trichoderma harzianum LTR-2 metabolites in the control of tobacco mosaic virus disease. The invention obtains a strain for controlling tobacco mosaic virus disease from Trichoderma spp. stored in laboratory, and carries out virus control research on the metabolite of the strain so as to directly and rapidly control tobacco mosaic virus disease, ensure tobacco yield and quality, and provide reference for maintaining microbial community balance in tobacco planting.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method and application of metabolites for preventing and treating tobacco mosaic virus disease, specifically an application of Trichoderma harzianum LTR-2. Background technique [0002] Tobacco is an important economic crop widely planted in northern and southern my country. The disease that affects the development of the tobacco industry is mainly tobacco mosaic virus disease. In recent years, the incidence of the disease has been serious year by year. The incidence rate in the field is generally 5-20%, and it can be as high as 90-100% in individual fields, resulting in a significant decline in the ratio of high-quality tobacco, unit yield and output value. Tobacco mosaic virus disease is caused by Tobacco mosaic virus (TMV), which can invade through tiny wounds caused by slight friction on adjacent leaves, and the main source of infection is the infected l...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 谢雪迎张新建朱常香王加宁温孚江周红姿
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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