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A high-performance liquid phase detection method for water-soluble vitamin E derivatives

A technology of water-soluble vitamins and high-efficiency liquid phase, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as inability to quantitatively analyze, and achieve the effects of short sample analysis time, high sensitivity and accuracy, and wide application prospects

Active Publication Date: 2022-02-15
上海联陆实业股份有限公司
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Problems solved by technology

[0011] In order to solve the problem in the prior art that the components after the esterification reaction of VES and PEG cannot be quantitatively analyzed, the purpose of the present invention is to provide a high-efficiency liquid phase detection method for water-soluble vitamin E derivatives, which not only It can quantitatively determine the specific content of TPGS monoester and diester, and can also measure the content of impurities in VES and PEG esterification products

Method used

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  • A high-performance liquid phase detection method for water-soluble vitamin E derivatives
  • A high-performance liquid phase detection method for water-soluble vitamin E derivatives
  • A high-performance liquid phase detection method for water-soluble vitamin E derivatives

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preparation example Construction

[0035] 1. Preparation of standard solution and sample solution

[0036] (a) Obtain the pure TPGS monoester and diester by simulated moving bed (SMB) chromatographic system, respectively weigh the pure TPGS monoester and diester, dissolve them with absolute ethanol, transfer to a volumetric flask, and wash with absolute ethanol Constant volume, obtain TPGS monoester and diester standard solution, store in -20 ℃ refrigerator, for use within 3 months;

[0037] (b) Preparation of α-tocopherol standard solution

[0038] Weigh the α-tocopherol reference substance, dissolve it with absolute ethanol, transfer it to a volumetric flask, and dilute to volume with absolute ethanol to obtain the α-tocopherol standard solution.

[0039] (c) Preparation of α-tocopheryl succinate standard solution

[0040] Weigh the α-tocopheryl succinate reference substance, dissolve it with absolute ethanol, transfer it to a volumetric flask, and use absolute ethanol to make up the volume to obtain the α-...

Embodiment 1

[0062] The water-soluble vitamin E derivative with batch number 161201 of our company was tested according to the above method, and the results were as follows: figure 1 As shown, by calculation, the content of each component is obtained: the content of TPGS monoester is 78.69%, the content of TPGS diester is 20.58%, the content of α-tocopherol is 0.15%, and the content of α-tocopherol succinate is 0.05% %.

Embodiment 2

[0064] The water-soluble vitamin E derivatives with batch number 170505 of our company were detected according to the above method, and the results were as follows: figure 2 As shown, by calculation, the content of each component is obtained: the content of TPGS monoester is 84.6%, the content of TPGS diester is 15.8%, the content of α-tocopherol is 0.08%, and the content of α-tocopherol succinate is 0.09% %.

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Abstract

The invention discloses a high-efficiency liquid phase detection method for water-soluble vitamin E derivatives, comprising the following steps: S1, preparing standards for TPGS monoester, TPGS diester, α-tocopherol, and α-tocopherol succinate with different concentrations S2, prepare sample solution; S3, inject the standard solution in S1 and the sample solution in S2 into the liquid chromatograph in sequence, the flow rate is 1.0 mL / min, the column temperature is 25-35°C, and the mobile phase is A mixture of acetonitrile and isopropanol; the high-efficiency liquid phase detection method for water-soluble vitamin E derivatives of the present invention can not only accurately analyze the content of TPGS monoester and diester, but also detect the residual VE and VES content in TPGS products, It is convenient to inspect and control product quality intuitively.

Description

technical field [0001] The invention relates to the technical field of chromatographic detection of vitamin E content, in particular to a high-efficiency liquid phase detection method of water-soluble vitamin E derivatives. Background technique [0002] RRR-α-tocopheryl polyethylene glycol succinate (also known as d -α-tocopheryl polyethylene glycol succinate, TPGS) is a water-soluble vitamin E derivative, and it is a new type of PEG carboxylate that has received extensive attention in recent years. Ester (VES) and polyethylene glycol (PEG1000) undergo an esterification reaction, and the product is a mixture of monoester and diester. TPGS contains both a lipophilic vitamin E structure and a hydrophilic PEG structure, and is a typical nonionic surfactant. TPGS has both the properties and physiological activities of tocopherol and PEG. In 1960, TPGS was found to be used as a solubilizer, and then reports on its toxicity and safety appeared one after another. In the 1980s, ...

Claims

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Application Information

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IPC IPC(8): G01N30/34G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N30/34
Inventor 潘斌蒋为群邬成华潘春花
Owner 上海联陆实业股份有限公司
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