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A Chlamydomonas strain and its application in biogas slurry purification

A technology of Chlamydomonas and algae strains, applied in the field of microorganisms, can solve the problems of secondary pollution, eutrophication, and high cost

Active Publication Date: 2021-04-30
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the development of large and medium-sized biogas projects also faces many environmental problems, among which the problem of how to treat and utilize the biogas slurry produced after anaerobic fermentation of livestock and poultry manure is particularly significant
The amount of biogas slurry produced by large and medium-sized biogas projects every day is very considerable. The biogas slurry that has only been anaerobically treated still contains a large amount of nutrients such as nitrogen and phosphorus. If it is directly discharged into the water body, it will easily cause eutrophication and cause secondary pollution.
At the same time, most large and medium-sized biogas projects are built on the outskirts of cities. It is difficult to consume biogas slurry on site, and the one-time consumption far exceeds the general law of crop fertilization. If it is transported over a long distance, it will consume a lot of energy and cost. high problem

Method used

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  • A Chlamydomonas strain and its application in biogas slurry purification
  • A Chlamydomonas strain and its application in biogas slurry purification
  • A Chlamydomonas strain and its application in biogas slurry purification

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: Separation, purification and preservation of algal strains

[0036] (1) Sample collection

[0037] The algal strains involved in the present invention are obtained by sampling and separating from the soil polluted by pig manure anaerobic digestion biogas slurry in the biogas service station of Xibaidian Village, Daxingzhuang Town, Pinggu District, Beijing. Soil samples were collected from the soil surface at selected locations, placed in sealed bags, and stored in a 4°C refrigerator for later use.

[0038] (2) Separation, purification and cultivation of algae strains

[0039]Take about 0.5 g of soil sample in a 24-well tissue culture dish, add BG11 liquid medium (the specific composition of BG11 medium is shown in Table 1) and place it in a light incubator with adjustable environmental parameters for algae enrichment culture. After culturing for 3-4 days, the color of the medium can be clearly seen to change from colorless to light green. Then on the BG...

Embodiment 2

[0050] Example 2 Exploration of Optimum Culture Conditions for Algae Strains

[0051] After obtaining the above-mentioned microalgae species with sufficient biomass, in order to explore its suitable culture conditions, the present invention carried out a single factor test on four common technical parameters of microalgae culture, light intensity, photoperiod, temperature and inoculum size—positive A series of optimization studies such as cross experiment—response surface experiment, etc. The parameters set are light intensity: 100, 150, 200, 250, 300 (μmol / ㎡ / s), photoperiod (light / dark ratio): 12 / 12, 14 / 10, 16 / 8, 18 / 6; temperature: 15°C, 20°C, 25°C, 30°C; pure culture inoculation amount (algae medium culture): 0.015g / L, 0.020g / L, 0.025g / L, 0.030g / L, found that the optimum is 0.025g / L. The same test method, set the inoculation amount of biogas slurry culture (algae species are used for the cultivation of pig manure biogas slurry): 0.020g / L, 0.030g / L, 0.040g / L, 0.050g / L, the...

Embodiment 3

[0054] In Example 3 and Comparative Example, 6 mL of culture fluid was sampled every day to measure biomass and water quality. All experimental groups were set up with three repetitions.

[0055] Example 3: Application of Chlamydomonas strain CGMCC NO.15497 to purify swine manure biogas slurry

[0056] (1) Test material

[0057] The pig manure biogas slurry used in this experiment was taken from the biogas slurry storage tank of the biogas service station in Xibaidian Village, Daxingzhuang Town, Pinggu District, Beijing, because the raw biogas slurry in the storage tank contained a relatively high concentration of ammonia nitrogen (>900mg / L) and a large amount of suspended solids (700-800 mg / L), the raw biogas slurry was pretreated by flocculation-air flotation, so that the concentration of ammonia nitrogen and suspended solids was reduced to the level that microalgae could tolerate. The water quality of the pretreated pig manure biogas slurry is shown in Table 2.

[0058] ...

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Abstract

The present invention proposes a strain of Chlamydomonas algae, the preservation number of which is: CGMCC NO.15497. The invention also proposes the application of the Chlamydomonas strain in biogas slurry purification. The Chlamydomonas whose preservation number is CGMCC NO.15497 proposed by the present invention can grow in pig manure biogas slurry under suitable culture conditions, using the pollutant ammonia nitrogen in pig manure biogas slurry as its own growth nitrogen source, and using pig manure The pollutant total phosphorus in the biogas slurry is used as a source of phosphorus for its own growth, and it plays a role in denitrification and phosphorus purification of pig manure biogas slurry. Compared with the Chlamydomonas strains purchased from other commercial algae species banks, the Chlamydomonas with the preservation number CGMCC NO.15497 of the present invention is no matter in the accumulation of biomass or in the effect of denitrification and phosphorus removal of swine manure and biogas slurry. have obvious advantages.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a Chlamydomonas and its application in biogas slurry purification. Background technique [0002] Using biogas engineering technology to treat poultry and livestock waste, while harvesting energy, realizes pollution control, can obtain multiple benefits such as environmental protection and economy, and is an effective means to solve the problem of livestock and poultry manure pollution. However, the development of large and medium-sized biogas projects also faces many environmental problems, among which the problem of how to treat and utilize the biogas slurry produced after anaerobic fermentation of livestock and poultry manure is particularly significant. Large and medium-sized biogas projects produce a considerable amount of biogas slurry every day. The biogas slurry that has only been anaerobically treated still contains a large amount of nutrients such as nitrogen an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F9/14C12R1/89
CPCC02F1/24C02F1/52C02F3/322C02F9/00C12N1/125C12R2001/89Y02E50/30
Inventor 卢海凤韩挺刘志丹张源辉段娜李保明
Owner CHINA AGRI UNIV
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