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A kind of chlorella and its application in biogas slurry purification

A chlorella and biogas slurry technology, applied in the field of microorganisms, can solve the problems of eutrophication, high energy consumption, and difficulty in absorbing biogas slurry on site.

Active Publication Date: 2021-01-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of large and medium-sized biogas projects also faces many environmental problems, among which the problem of how to treat and utilize the biogas slurry produced after anaerobic fermentation of livestock and poultry manure is particularly significant
The amount of biogas slurry produced by large and medium-sized biogas projects every day is very considerable. The biogas slurry that has only been anaerobically treated still contains a large amount of nutrients such as nitrogen and phosphorus. If it is directly discharged into the water body, it will easily cause eutrophication and cause secondary pollution.
At the same time, most large and medium-sized biogas projects are built in the outskirts of the city. It is difficult to consume biogas slurry on the spot, and the one-time consumption far exceeds the general law of crop fertilization. If it is transported over a long distance, there will be high energy consumption and cost high problem

Method used

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  • A kind of chlorella and its application in biogas slurry purification
  • A kind of chlorella and its application in biogas slurry purification
  • A kind of chlorella and its application in biogas slurry purification

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: Separation and purification of algal strains

[0035] (1) Sample collection

[0036] The algal strains involved in the present invention are obtained by sampling and separating from the soil polluted by pig manure anaerobic digestion biogas slurry in the biogas service station of Xibaidian Village, Daxingzhuang Town, Pinggu District, Beijing. Soil samples were collected from the soil surface at selected locations, placed in sealed bags, and stored in a 4°C refrigerator for later use.

[0037] (2) Separation, purification and cultivation of algae strains

[0038]Take about 0.5 g of soil sample in a 24-well tissue culture dish, add BG11 liquid medium (the specific composition of BG11 medium is shown in Table 1) and place it in a light incubator with adjustable environmental parameters for algae enrichment culture. After culturing for 3-4 days, the color of the medium can be clearly seen to change from colorless to light green. Then on the BG11 solid plate...

Embodiment 2

[0042] Embodiment 2 research on the optimum culture condition of algae strain

[0043] After obtaining the above-mentioned microalgae species with sufficient biomass, in order to explore its suitable culture conditions, the present invention carried out a single factor test on four common technical parameters of microalgae culture, light intensity, photoperiod, temperature and inoculum size—positive A series of optimization studies such as cross test-response surface test, the value range of each parameter is: light intensity: 50μmol / m 2 / s~250μmol / m 2 / s; Photoperiod: (12~20) / (4~12); Temperature: 20°C-30°C; Inoculation amount: 0.04~0.15g / L.

[0044] Finally, the most suitable culture condition for this strain of microalgae is that the light intensity is 140 μmol / m 2 / s, the photoperiod is 19:5 (light:dark), the temperature is 26±0.5°C, and the inoculum volume is 0.058g / L. The culture vessel used in this stage is a 250mL Erlenmeyer flask (effective culture volume is 150mL), ...

experiment example

[0045] Experimental example: identification and preservation of algae strains

[0046] The identification of the algae strains was carried out in two steps, firstly the preliminary morphological observation, and then the molecular biological identification. The isolated and purified microalgae strains were observed and photographed using an optical microscope (10x eyepiece × 40x objective). Observe the cell shape, size, structure and other characteristics of the algae strain. The morphology of algal strains observed under the optical microscope is as follows: figure 1 shown.

[0047] The strain of microalgae obtained by separation and purification of the present invention is observed under a microscope, and the algae body is green, and it is a single-cell population, and the cells are oval. Molecular biological identification of algae strains was carried out, using DNAMAN and Primer 5.0 software to design primers using the extracted DNA as a template (primer sequence forwar...

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Abstract

The invention provides a chlorella whose preservation number is CGMCC NO.15496. The present invention also proposes the application of the chlorella, which is applied to the purification of biogas slurry. The chlorella proposed by the present invention can grow in pig manure biogas slurry under suitable culture conditions, using the pollutant ammonia nitrogen in pig manure biogas slurry as its own growth nitrogen source, and using the pollutants in pig manure biogas slurry As a source of phosphorus for its own growth, total phosphorus plays a role in denitrification and phosphorus purification of swine manure biogas slurry. Compared with the chlorella strains purchased from other commercial algae species banks, the chlorella has obvious advantages in terms of biomass accumulation and denitrification and phosphorus removal of swine manure and biogas slurry.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a chlorella and an application thereof. Background technique [0002] In recent years, with the rapid development of intensive production of livestock and poultry farming worldwide, the pollution of livestock and poultry manure has become increasingly serious. The Food and Agriculture Organization of the United Nations has listed intensive livestock and poultry farming as one of the three major pollution sources of world environmental problems. . The use of biogas engineering technology to treat livestock waste can not only control pollution, but also realize the utilization of waste resources, and can harvest multiple benefits such as energy, environmental protection and economy, which is in line with the concept of sustainable development of human society. However, the development of large and medium-sized biogas projects also faces many environmental problems, among ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89
CPCC02F3/322C12N1/125C12R2001/89Y02E50/30
Inventor 卢海凤韩挺刘志丹张源辉段娜李保明
Owner CHINA AGRI UNIV
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