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Two SNP markers closely linked to cucumber powdery mildew resistance and application thereof

A technology against powdery mildew and powdery mildew, applied in the fields of genetic engineering and molecular biology, to achieve the effects of solving the harm, close to fine positioning, and high sensitivity

Active Publication Date: 2019-01-18
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing reports mainly focus on the identification of powdery mildew resistance QTLs, and there are relatively few reports on the use of SNP markers for assisted selection of cucumber breeding traits

Method used

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  • Two SNP markers closely linked to cucumber powdery mildew resistance and application thereof
  • Two SNP markers closely linked to cucumber powdery mildew resistance and application thereof
  • Two SNP markers closely linked to cucumber powdery mildew resistance and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Construction of genetic segregation populations

[0031] 1. Secondary F 2 Group construction

[0032] D8 (male parent) is a dwarf line highly susceptible to powdery mildew. Quoted from the University of North Carolina in the United States. Its stem is thick and thick. When the plant grows to 12-14 nodes, it will be capped with a cluster of female flowers and stop growing. The leaves and peel are dark green, the fruit is thick and short, and the flesh is thick; SSL508-27 ( The female parent) is a cucumber line Jin5-508 highly resistant to powdery mildew and a susceptible line D8 of powdery mildew were used as parents in the early stage of the laboratory, and D8 was used as the recurrent parent for 11 consecutive generations of backcrossing. Raw and disease-resistant plants were subsequently screened with SCAR markers to aid in the selection of resistant strains, and then selfed to obtain chromosome segment substitution lines with D8 as the genetic background but phe...

Embodiment 2

[0053] (1) Select 8 cucumber varieties known to be resistant to powdery mildew, of which 4 are resistant to powdery mildew (Jin5-508, XUE1, BGF, Riyin2 ), and 4 are susceptible to powdery mildew (EP6392, superina, ZK, Axin).

[0054] (2) Extract the genomic DNA of the above eight varieties and their parents respectively, use the dCAPS markers derived from pm5_01 and pm5_02 to perform PCR amplification, and then use the restriction endonuclease DdeI to digest the PCR product amplified by the pm5_01 primer; usage restrictions The PCR product amplified by the pm5_02 primer was digested by the endonuclease MnlI;

[0055] (3) The results of polyacrylamide gel electrophoresis showed that the identification of disease resistance was consistent with the results of double enzyme digestion ( Figure 5 , Image 6 ), the above two SNP markers can distinguish powdery mildew resistant from susceptible powdery mildew varieties.

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Abstract

The invention relates to two SNP markers closely linked to cucumber powdery mildew resistance in the field of genetic engineering and the application thereof, and provides two SNP markers closely linked to cucumber powdery mildew resistance QTL, respectively named pm5_01 and pm5_02. Pm5_01 is located at chromosome 16,786,038 and pm5_02 is located at chromosome 16,996,485 in cucumber. The inventionutilizes the methods of variomics and population genetics to screen SNP markers closely linked to cucumber powdery mildew resistance, identifies the cucumber genotype by sequencing and converting into dCAPS markers, and judges the difference of cucumber powdery mildew resistance according to the genotype, thereby accelerating the selection and breeding process of cucumber powdery mildew resistance varieties. At the same time, these two SNP markers also laid an important foundation for further development of molecular marker-assisted breeding for powdery mildew resistance, cloning of resistance genes and selection of new powdery mildew resistant cucumber varieties.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and molecular biology, and relates to two SNP markers closely linked with cucumber powdery mildew resistance and an application thereof. Background technique [0002] Cucumbers are Cucurbitaceae ( Cucurbitaceae ) Melon ( Cucumis) Annual vines are one of the ten most important vegetable crops in the world and one of the main vegetable crops in my country. Powdery mildew is one of the main diseases in cucumber production worldwide. When the disease occurs, the photosynthesis of leaves is weakened, which often causes serious yield, quality and economic losses. Cucumber powdery mildew is a fungal disease, and the pathogen is an obligate parasite ( Podosphaera xanthii ), mainly infecting leaves and stems. Powdery mildew can occur in all stages of cucumber growth. When the disease occurs, the growth of the plants is weak, and the fruit grows slowly in the later stage, which may lead to a reduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈学好王维许学文齐晓花徐强
Owner YANGZHOU UNIV
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