PH (Potential of Hydrogen) response antibacterial peptide as well as preparation method and application thereof

Abstract

The invention discloses pH (Potential of Hydrogen) response antibacterial peptide as well as a preparation method and application thereof. The invention firstly provides the antibacterial peptide witha simple amino acid sequence and the amino acid sequence only comprises hydrophilic lysine and hydrophobic leucine, but has key factors with an antibacterial effect of natural antibacterial peptide:positive charge, a hydrophobic domain, amphipathy, alpha helix secondary conformation and the like; the end part of the antibacterial peptide is simply modified and the bioactivity of the antibacterial peptide can be maintained; and meanwhile, the biocompatibility is improved. According to the pH response antibacterial peptide provided by the invention, an acid response molecule is modified on anantibacterial polypeptide chain, and the polypeptide can have a sterilization effect in a slightly-acidic environment of a bacterial infection part and also has the effect of reducing the toxicity ofthe antibacterial peptide on mammalian cells in a physiological environment.

Description

Technical field [0001] The invention relates to the technical field of antibacterial peptides. More specifically, it relates to a pH-responsive antibacterial peptide and its preparation method and application. Background technique [0002] In recent years, more and more pathogenic bacteria have developed resistance to antibiotics, and there is an urgent need to develop new antibacterial agents. Antimicrobial peptides (AMPs) are peptides with antibacterial activity in organisms. The number of amino acids is usually 10-50, and they are often positively charged and have broad-spectrum antibacterial activity. AMPs are an important part of the innate non-specific defense system of most organisms against invading pathogens. They have a unique antibacterial action mechanism, quickly kill bacteria and are not easy to cause bacterial resistance. They can be used alone or in combination with antibiotics to kill pathogens. Traditional antibiotics usually target a single enzyme to control ...

AI Technical Summary

Problems solved by technology

For example, grafting polyethylene glycol (PEG) to the N-terminus of the antimicrobial peptide CaLL can improve the compatibility of the antimicrobial peptide with lung tissue, but at the same time the antibacterial effect is reduced by 50% (Morris, C.J.Antimicrob.Agents Chemother. 2012,56,3298-3308.); Grafting cationic polypeptide KSLW onto PEG-modified phospholipid micelles can also improve cytocompatibility while maintaining antibacterial activity (Lee, W. Theranostics2017, 7, 3759-3767. ), design and synthesize artificial antimicrobial peptides and their derivatives to reduce cytotoxicity and maintain antibacterial activity (G.N.Tew Acc.Chem.Res.2010,43,30.)

Images